Reviewed, UniProtKB/Swiss-Prot Q0VRL1 (SYP_ALCBS)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	ABO_0739

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000288307

Sequences

	Sequence		Length	Mass (Da)
	Q0VRL1-1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: D607635BD730C181	FASTA	572	63,117
	10 20 30 40 50 60 MRTSQYLLAT VKETPADAVV TSHQLMLRAG MVRKLASGLY TWLPSGLRVL RKVEQVIREE 70 80 90 100 110 120 MDRAGAQEVL MPVVQPMELW EESGRAPAYG PELLRITDRH NNPFCLGPTH EEVITDMVRN 130 140 150 160 170 180 ELHSYKQLPA NFYQIQTKFR DEIRPRFGIM RSREFIMKDA YSFHTNMDSL ADTYQIMHRA 190 200 210 220 230 240 YCAIFDRLGL DYRPVEADTG AIGGAASHEF HVLAESGEDD IAFSDGSDYA ANVELAEALA 250 260 270 280 290 300 PTSERPAPGA AIEKVDTPNA KTIEELVTQF NLPIEKTIKT LVVKGAEEGQ LVALLVRGDH 310 320 330 340 350 360 ELNDIKAEKL DAVATPLEFA SEEEIRKAVG AGPGSLGPVD LPFTVIADRS VAVMSDFGAG 370 380 390 400 410 420 ANEDGKHYFN INWERDVALP QIADLRNVVD GDPSPDGSGT LTIKRGIEVG HIFQLGTKYS 430 440 450 460 470 480 EALGATVLDE NGKSVVMPMG CYGIGVTRVV AAAIEQNNDD RGIIWPEAIA PFQVALVPVN 490 500 510 520 530 540 IKKSPREREL AEKLYAELTA MGIEVLFDDR EKERLGVKLA DTELLGIPHR IVIAERGMDN 550 560 570 GVLEYKGRRD ADKTEVAMDE IVAFLKEKMG MN

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL16187.1. Different initiation.
RefSeq	YP_692459.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211222.
GenomeReviews	Gene locus ABO_0739 in contig AM286690_GR.
KEGG	abo:ABO_0739.
NMPDR	fig|393595.12.peg.742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VRL1.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0739-MON.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ALCBS

Accession

Primary (citable) accession number: Q0VRL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)