Reviewed, UniProtKB/Swiss-Prot Q0VR89 (METE_ALCBS)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	ABO_0861

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017217
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VR89-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: C69694CA7A5E94A8	FASTA	763	86,266
	10 20 30 40 50 60 MTTLHNLGFP RIGARRELKQ AQEAYWAGDL QQSELEHVGK SLRERHWALQ ADAGVDLMPV 70 80 90 100 110 120 GDFAWYDHIL EFSSLLGVVP SRFEQDANAP VNLDTLFRMA RGRAPTGTPA AACEMTKWFD 130 140 150 160 170 180 TNYHYLVPEL AADQTFRIAR ESLFEQVEEA KAQGHNPKPV IPGPLTYLYL SKGDRFEGAE 190 200 210 220 230 240 DSAKLALLDN LIPVYREILQ RFANQGVQWV QIDEPILVLD LPDSWQRAYL RVYDQLASAS 250 260 270 280 290 300 QTKLLLATYF GGLGNNLFTA LELPVHGLHL DRVRGNDDLG QVIPRLGDKV LSLGVINGRN 310 320 330 340 350 360 IWRTDLDAAL DNITALHNEL GDRLWLAPSC SLLHCPVDLE QEDTLDNELK SWLSFAKQKL 370 380 390 400 410 420 EELALLGGAL RGNSNVNSGL QTQRAALQAR GLSSRIHNPA VAQRLADASQ LSRDRVSPFA 430 440 450 460 470 480 ERIAQQQHAL QLPAFPTTTI GSFPQTREIR TARRDWKAGK LNDAQYQQQM QEEIARCIRY 490 500 510 520 530 540 QEEVELDVLV HGEAERNDMV EYFGELLDGF AFTRFGWVQS YGSRCVKPPI IFGDVQRPQA 550 560 570 580 590 600 MTVEWARYAQ SLTHKPVKGM LTGPVTILQW SFVRDDQPRA DTCRQIALAL RDEVQDLEKA 610 620 630 640 650 660 GIKVIQIDEP ALREGLPLRQ EEWTQYLDWA VDCFRLATVS VDDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 EAIAALDADV ITIETSRSNM ELLDAFRDFH YPNDIGPGVY DIHSPNEPDV AWMVQLMEKA 730 740 750 760 AERLPKERLW VNPDCGLKTR KWDETRAALA NMVNAAKQLR QAS

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL16309.1.
RefSeq	YP_692581.1.
3D structure databases
SMR	Q0VR89. Positions 4-761.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VR89.
Genome annotation databases
GeneID	4211501.
GenomeReviews	Gene locus ABO_0861 in contig AM286690_GR.
KEGG	abo:ABO_0861.
NMPDR	fig|393595.12.peg.864.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	SFVRNDI.
PhylomeDB	Q0VR89.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_0861-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_ALCBS

Accession

Primary (citable) accession number: Q0VR89

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)