Siroheme synthase - Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573)

Contribute

Send feedback

Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot Q0VQ05 (CYSG_ALCBS)

Last modified June 16, 2009. Version 29. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Siroheme synthase
Including the following 3 domains:
    1- Recommended name:
            Uroporphyrinogen-III C-methyltransferase
                Short name=Urogen III methylase
              EC=2.1.1.107
        Alternative name(s):
            SUMT
            Uroporphyrinogen III methylase
              Short name=UROM
    2- Recommended name:
            Precorrin-2 dehydrogenase
              EC=1.3.1.76
    3- Recommended name:
            Sirohydrochlorin ferrochelatase
              EC=4.99.1.4

Gene names

Name:	cysG
Ordered Locus Names:	ABO_1295

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Hide | Top

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

Hide | Top

General annotation (Comments)

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity.
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646 S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646 Precorrin-2 + NAD⁺ = sirohydrochlorin + NADH. HAMAP MF_01646 Siroheme + 2 H⁺ = sirohydrochlorin + Fe²⁺. HAMAP MF_01646
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Hide | Top

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	NAD S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW siroheme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: HAMAP sirohydrochlorin ferrochelatase activity Inferred from electronic annotation. Source: EC uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 461

461

Siroheme synthase HAMAP MF_01646

PRO_0000330491

Regions

Region

220 – 461

242

Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q0VQ05-1 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: EA11471035C5EA8A
Show »

FASTA

461

49,741

        10         20         30         40         50         60 
MEFLPISWRL QGKVALLAGG GEVALRKGRL LHRSGAILTV VAPQVCDELL DIVVRSGGSC 

        70         80         90        100        110        120 
VVRAFESADL DGVALVICAT DDRSTNAEIA KQAQQRGLPV NVVDDPSLGD FIFPAIVDRS 

       130        140        150        160        170        180 
PVLISISSSG ASPVLARKLR AQLESTLPAR WGRLADLMAR FRQPLKDKLS NIGARRLFWE 

       190        200        210        220        230        240 
QTLDSPVVEK VLAGKDSEAE AMLAAAIESA DATTLSRGEV YLVGAGPGDP DLLTFRALRL 

       250        260        270        280        290        300 
LQKADVVLYD RLVGKGIVDL ARRDAELVYV GKARDKHALP QDNINELLVH YAKQGKKVCR 

       310        320        330        340        350        360 
LKGGDPFIFG RGGEEIDLIV AEGIDFQVVP GITAASGCAS YAGIPLTHRD HAQSVRFVTG 

       370        380        390        400        410        420 
HRKDGSVDLD WKHLVSETET VVFYMGLVGL REICSQLIAH GRGGDTPIAL VSRGTTNLQE 

       430        440        450        460 
VITGRLDQLP DDIEGREIHA PTLIIVGSVV SLHPKFGWFK P

« Hide

Hide | Top

Cross-references

Sequence databases
	AM286690 Genomic DNA. Translation: CAL16743.1.
RefSeq	YP_693015.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4212573.
GenomeReviews	Gene locus ABO_1295 in contig AM286690_GR.
KEGG	abo:ABO_1295.
NMPDR	fig\|393595.12.peg.1307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VQ05.
OMA	Q0VQ05. MCRRDAE.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_1295-MON.
Family and domain databases
HAMAP	MF_01646. [Tree]
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA_cysG_C. IPR016040. NAD(P)-bd_dom. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit. G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF10414. CysG_dimeriser. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01469. cobA_cysG_Cterm. 1 hit. TIGR01470. cysG_Nterm. 1 hit.
PROSITE	PS00839. SUMT_1. False negative. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

CYSG_ALCBS

Accession

Primary (citable) accession number: Q0VQ05

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents