Reviewed, UniProtKB/Swiss-Prot Q0VP51 (SYM_ALCBS)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	ABO_1599

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331776
Regions
	Domain	584 – 686	103	tRNA-binding
	Motif	22 – 32	11	"HIGH" region HAMAP MF_00098
	Motif	337 – 341	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	153	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	166	1	Zinc By similarity
	Metal binding	169	1	Zinc By similarity
	Binding site	340	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VP51-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 12B533D19987EF81	FASTA	686	76,792
	10 20 30 40 50 60 MEIDAVPMSD TPRKILVTSA LPYANGPIHL GHLLEYIQTD IWVRFQKLRG QQCIYVCADD 70 80 90 100 110 120 AHGTAIMLKA EENGVTPEQQ IAQVKADHER DFADFQVNFD NYYSTHSPEN RALSEMVFQR 130 140 150 160 170 180 NKDAGYILEK TITQLYDPQK GMFLADRFVK GTCPKCKSED QYGDNCEVCG ATYTPAELIE 190 200 210 220 230 240 PYSSVSGATP VEKETTQLFF DLPKFEALLR EWTRSGALQE QVANKLQEWI EDLQPWDISR 250 260 270 280 290 300 EAPYFGFEIP GYPGKYFYVW LDAPIGYMAS FQNYCDREGL SFDDYWGKES TAELYHFIGK 310 320 330 340 350 360 DIINFHGLFW PAMLDGAGLR KPTAIYSHGF VTVNGAKMSK SRGTFIKART YLDHLSPEYL 370 380 390 400 410 420 RYYFAAKLSS RVDDFDLNLE DFAQRVNSDL VGKLVNIASR TGNFVKKFGG TLSTELDNMM 430 440 450 460 470 480 LVREVQEAGP RIADFYEKRE FGKAMREIMA LADHANVYIA DKAPWSLSKE EGKEQEVLAI 490 500 510 520 530 540 CSTAINVFRL LVIYLKPVLP ALAERAEAFM QVEPLTWADS EQLLLGHEIA KFKPLLSRVD 550 560 570 580 590 600 MAQVESMLED SKESTPAPAA AKPKKAATQK ADAADNDETI SIDDFLKVKL RVARVAKAAH 610 620 630 640 650 660 VDGADKLLQL TLDVGELGQR NVFAGIKARY APEELEGKLV VLVANLAPRK MKFGISEGMV 670 680 LAAGPGGDDI HILSPDSGAE PGMEVR

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL17047.1.
RefSeq	YP_693319.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211503.
GenomeReviews	Gene locus ABO_1599 in contig AM286690_GR.
KEGG	abo:ABO_1599.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VP51.
OMA	Q0VP51. CEAVPES.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_1599-MON.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_ALCBS

Accession

Primary (citable) accession number: Q0VP51

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)