Reviewed, UniProtKB/Swiss-Prot Q0VNK2 (SYA_ALCBS)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	ABO_1798

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 863	863	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347483

Sequences

	Sequence		Length	Mass (Da)
	Q0VNK2-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: E7D0C86144E21574	FASTA	863	93,888
	10 20 30 40 50 60 MKSAELRQAF LDYFASQGHT KVSSSSLVPA NDPTLLFTNA GMNQFKDVFL GRESRDYTRA 70 80 90 100 110 120 TSSQRCVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKREAIKFAW EFLTGTLGLP 130 140 150 160 170 180 EERLWVTVHV SDDEAADIWL KEMGVSAERF SRLDEDNFWQ MGDTGPCGPS SEIFYDHGAD 190 200 210 220 230 240 VPGGPPGSAD EDLDRYIEIW NLVFMQYDRQ PDGELQPLPK PSVDTGMGLE RIAAVLQGVH 250 260 270 280 290 300 SNYEIDLFQA LLQAAAKATG CTDLEEKSLR VIADHIRSAS FLICDGVIPS NEGRGYVLRR 310 320 330 340 350 360 IIRRALRHGH KLGQDKPFFS TLVAALVAEM GEAYPELGRE QARIEKALLA EEEQFGRTLA 370 380 390 400 410 420 AGMKVLESAI EQLDGRVLPG DVLFNLYDTH GFPPDLTADV ARERDLSVDM DGFEAAMAAQ 430 440 450 460 470 480 RERARGAGSF ANDYSDRLNI DAITDFSGYE KLADDNTVVA LYKDGAAVDT LNAGEEGMVV 490 500 510 520 530 540 LDRTPFYAES GGQVGDTGAL IADEARFMVT DTRKRQAAHV HVGKQVSGSL TIGGKVSACV 550 560 570 580 590 600 DVDRRLAVMR NHSATHLMHA ALRNVLGEHV QQKGSLVSAD YLRFDFSHGE AVSEAQREEI 610 620 630 640 650 660 EILVNRQILA NTAVTTELMD IESAREAGAM ALFGEKYDDQ VRVLSMGSDG FSKELCGGTH 670 680 690 700 710 720 VNRTGDIGLF RITLEASAAA GVRRIEAVTG EHALAVMRRQ ERALSEIAAT VKSSLDNAAD 730 740 750 760 770 780 RVRSQAQKVR ELEKEIERLK QKLASGAGGD LTSEVQDING VKVLATQIDG ADGKTLRVTM 790 800 810 820 830 840 DKLKDKLGSA VIVLAAVEGE KVALVAGVTK DLTTKYKAGD LLKFVAEQVD GRGGGRPDMA 850 860 QGGGNNPAAL PGALESVKAW VAE

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL17246.1. Different initiation.
RefSeq	YP_693518.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211293.
GenomeReviews	Gene locus ABO_1798 in contig AM286690_GR.
KEGG	abo:ABO_1798.
NMPDR	fig|393595.12.peg.1802.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VNK2.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_1798-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ALCBS

Accession

Primary (citable) accession number: Q0VNK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)