Reviewed, UniProtKB/Swiss-Prot Q0VNG2 (SYFA_ALCBS)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	ABO_1838

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000006796
Sites
	Metal binding	253	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VNG2-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 92703B603C38344C	FASTA	339	37,628
	10 20 30 40 50 60 MENLNALVDA ALAEVEQAGD IRALDDVRVK YLGKKGEISA LMKGLGKLSA EERPQAGAVI 70 80 90 100 110 120 NEGKQKVQDA IADRKSAMEE AALKQQLASE TVDVTLPGRG ELPGGLHPVN RMRRRIEDFF 130 140 150 160 170 180 LRLGFDISEG PEVEDDFHNF EALNIPSHHP ARAMHDTFYF GDGRLLRTHT SPVQVRVMEQ 190 200 210 220 230 240 GKPPFRIIAP GRVYRCDSDL THTPMFHQVE GLLVDKGITF ADLRGTVAEF LRYTFEVEDL 250 260 270 280 290 300 PVRFRPSYFP FTEPSAEVDV GCVSCGGEGC RVCSHTGWIE IMGCGMVHPT VLEAGGVDAE 310 320 330 EYSGFAFGMG IERIAMLRYG VNDLRLFFEN DTRFLSQFG

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL17286.1.
RefSeq	YP_693558.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211602.
GenomeReviews	Gene locus ABO_1838 in contig AM286690_GR.
KEGG	abo:ABO_1838.
NMPDR	fig|393595.12.peg.1844.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VNG2.
OMA	Q0VNG2. FRASYFP.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_1838-MON.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR002319. Phe-tRNA-synth_IIc-rel. IPR004529. Phe-tRNA-synth_IIc_asu. IPR018157. Phe-tRNA-synth_IIc_C. IPR004188. Phe-tRNA_synth_II_N. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ALCBS

Accession

Primary (citable) accession number: Q0VNG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)