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Q0VN53 (SYL_ALCBS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:ABO_1947
OrganismAlcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]
Taxonomic identifier393595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesAlcanivoracaceaeAlcanivorax

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence CAL17395.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 815815Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334726

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif574 – 5785"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5771ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0VN53 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 4B1E6B8ECD966DBA

FASTA81591,315
        10         20         30         40         50         60 
MDVQYRPDQI EAQAQQYWDD NQSFKVTEDA SKEKFYCLSM FPYPSGRLHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVSRYQRMLG KNVLQPMGWD AFGLPAENAA IKNKVAPAKW TFENIDYMRG QLQRLGFGYD 

       130        140        150        160        170        180 
WGRELATCTP EYYRWEQWFF TKLYEKGLVY RKMSTVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEQKEIPQ WFIKITDYAD ELLNDLDQLD GWPEQVKTMQ RNWIGRSEGV ELDFPIEGEE 

       250        260        270        280        290        300 
SLRVYTTRPD TLMGVSYVAV AAGHPLAQKA AAANHEVADF IKECQNTKTA EADMATMEKK 

       310        320        330        340        350        360 
GIYTGLTATH PISGEAVPVW IANFVLMGYG TGAVMAVPAH DQRDFEFAQK YGLPINQVIE 

       370        380        390        400        410        420 
PANGEPIDLA LEAFTGKGTL IHSGEFDGLS SAEAFNAIAN WLSERSLGEK KVNYRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGTP IPMVETEDGT LHPTPEDQLP VALPTDVEMD GVTSPIKADP EWAKTTFNGQ 

       490        500        510        520        530        540 
PALRETDTFD TFMESSWYYA RYCSPQSDTA MLDPAATNYW LPVDQYIGGI EHAILHLLYS 

       550        560        570        580        590        600 
RFFHKLLRDT GLVNCDEPFK QLLCQGMVLK DGAKMSKSKG NTVDPQQMIE EYGADTVRLF 

       610        620        630        640        650        660 
MMFAAPPEQS LEWNDAGVEG AFRFIKRLWR LVAEHVEAGN TGTLDVNALD DAGKALRRKT 

       670        680        690        700        710        720 
HETIQKVSDD YGRRNTFNTA IAAVMELINE VSKFDAATDN ALAVKQEALE AAVLLLAPII 

       730        740        750        760        770        780 
PHAGHSLWQA LGHDEAVIDA SWPSVDESAL VKDSIELVVQ VNGKVRAKLN VPANADKASV 

       790        800        810 
ESLAMDEPNV KKFTEGKTVR KVIVVPGKLV NIVAN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM286690 Genomic DNA. Translation: CAL17395.1. Different initiation.
RefSeqYP_693667.1. NC_008260.1.

3D structure databases

ProteinModelPortalQ0VN53.
SMRQ0VN53. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING393595.ABO_1947.

Proteomic databases

PRIDEQ0VN53.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL17395; CAL17395; ABO_1947.
GeneID4211542.
KEGGabo:ABO_1947.
PATRIC20841731. VBIAlcBor124741_2039.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycABOR393595:GHRI-1991-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ALCBS
AccessionPrimary (citable) accession number: Q0VN53
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries