Reviewed, UniProtKB/Swiss-Prot Q0VN53 (SYL_ALCBS)

Names and origin

Protein names

Recommended name:
    Leucyl-tRNA synthetase
    EC=6.1.1.4
Alternative name(s):
    Leucine--tRNA ligase
      Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	ABO_1947

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP MF_00049
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 815	815	Leucyl-tRNA synthetase HAMAP MF_00049		PRO_0000334726
Regions
	Motif	42 – 52	11	"HIGH" region HAMAP MF_00049
	Motif	574 – 578	5	"KMSKS" region HAMAP MF_00049
Sites
	Binding site	577	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VN53-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 4B1E6B8ECD966DBA	FASTA	815	91,315
	10 20 30 40 50 60 MDVQYRPDQI EAQAQQYWDD NQSFKVTEDA SKEKFYCLSM FPYPSGRLHM GHVRNYSIGD 70 80 90 100 110 120 VVSRYQRMLG KNVLQPMGWD AFGLPAENAA IKNKVAPAKW TFENIDYMRG QLQRLGFGYD 130 140 150 160 170 180 WGRELATCTP EYYRWEQWFF TKLYEKGLVY RKMSTVNWDP VDQTVLANEQ VIDGRGWRSG 190 200 210 220 230 240 ALVEQKEIPQ WFIKITDYAD ELLNDLDQLD GWPEQVKTMQ RNWIGRSEGV ELDFPIEGEE 250 260 270 280 290 300 SLRVYTTRPD TLMGVSYVAV AAGHPLAQKA AAANHEVADF IKECQNTKTA EADMATMEKK 310 320 330 340 350 360 GIYTGLTATH PISGEAVPVW IANFVLMGYG TGAVMAVPAH DQRDFEFAQK YGLPINQVIE 370 380 390 400 410 420 PANGEPIDLA LEAFTGKGTL IHSGEFDGLS SAEAFNAIAN WLSERSLGEK KVNYRLRDWG 430 440 450 460 470 480 VSRQRYWGTP IPMVETEDGT LHPTPEDQLP VALPTDVEMD GVTSPIKADP EWAKTTFNGQ 490 500 510 520 530 540 PALRETDTFD TFMESSWYYA RYCSPQSDTA MLDPAATNYW LPVDQYIGGI EHAILHLLYS 550 560 570 580 590 600 RFFHKLLRDT GLVNCDEPFK QLLCQGMVLK DGAKMSKSKG NTVDPQQMIE EYGADTVRLF 610 620 630 640 650 660 MMFAAPPEQS LEWNDAGVEG AFRFIKRLWR LVAEHVEAGN TGTLDVNALD DAGKALRRKT 670 680 690 700 710 720 HETIQKVSDD YGRRNTFNTA IAAVMELINE VSKFDAATDN ALAVKQEALE AAVLLLAPII 730 740 750 760 770 780 PHAGHSLWQA LGHDEAVIDA SWPSVDESAL VKDSIELVVQ VNGKVRAKLN VPANADKASV 790 800 810 ESLAMDEPNV KKFTEGKTVR KVIVVPGKLV NIVAN

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL17395.1. Different initiation.
RefSeq	YP_693667.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4211542.
GenomeReviews	Gene locus ABO_1947 in contig AM286690_GR.
KEGG	abo:ABO_1947.
NMPDR	fig|393595.12.peg.1948.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VN53.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_1947-MON.
Family and domain databases
HAMAP	MF_00049. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-synth_Ia_bac/mito. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF7. Leu_tRNAsyn_1a. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_ALCBS

Accession

Primary (citable) accession number: Q0VN53

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)