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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciABOR393595:GHRI-1991-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:ABO_1947
OrganismiAlcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Taxonomic identifieri393595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesAlcanivoracaceaeAlcanivorax
ProteomesiUP000008871 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815Leucine--tRNA ligasePRO_0000334726Add
BLAST

Proteomic databases

PRIDEiQ0VN53.

Interactioni

Protein-protein interaction databases

STRINGi393595.ABO_1947.

Structurei

3D structure databases

ProteinModelPortaliQ0VN53.
SMRiQ0VN53. Positions 228-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi574 – 5785"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0VN53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVQYRPDQI EAQAQQYWDD NQSFKVTEDA SKEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYSIGD VVSRYQRMLG KNVLQPMGWD AFGLPAENAA IKNKVAPAKW
110 120 130 140 150
TFENIDYMRG QLQRLGFGYD WGRELATCTP EYYRWEQWFF TKLYEKGLVY
160 170 180 190 200
RKMSTVNWDP VDQTVLANEQ VIDGRGWRSG ALVEQKEIPQ WFIKITDYAD
210 220 230 240 250
ELLNDLDQLD GWPEQVKTMQ RNWIGRSEGV ELDFPIEGEE SLRVYTTRPD
260 270 280 290 300
TLMGVSYVAV AAGHPLAQKA AAANHEVADF IKECQNTKTA EADMATMEKK
310 320 330 340 350
GIYTGLTATH PISGEAVPVW IANFVLMGYG TGAVMAVPAH DQRDFEFAQK
360 370 380 390 400
YGLPINQVIE PANGEPIDLA LEAFTGKGTL IHSGEFDGLS SAEAFNAIAN
410 420 430 440 450
WLSERSLGEK KVNYRLRDWG VSRQRYWGTP IPMVETEDGT LHPTPEDQLP
460 470 480 490 500
VALPTDVEMD GVTSPIKADP EWAKTTFNGQ PALRETDTFD TFMESSWYYA
510 520 530 540 550
RYCSPQSDTA MLDPAATNYW LPVDQYIGGI EHAILHLLYS RFFHKLLRDT
560 570 580 590 600
GLVNCDEPFK QLLCQGMVLK DGAKMSKSKG NTVDPQQMIE EYGADTVRLF
610 620 630 640 650
MMFAAPPEQS LEWNDAGVEG AFRFIKRLWR LVAEHVEAGN TGTLDVNALD
660 670 680 690 700
DAGKALRRKT HETIQKVSDD YGRRNTFNTA IAAVMELINE VSKFDAATDN
710 720 730 740 750
ALAVKQEALE AAVLLLAPII PHAGHSLWQA LGHDEAVIDA SWPSVDESAL
760 770 780 790 800
VKDSIELVVQ VNGKVRAKLN VPANADKASV ESLAMDEPNV KKFTEGKTVR
810
KVIVVPGKLV NIVAN
Length:815
Mass (Da):91,315
Last modified:May 20, 2008 - v2
Checksum:i4B1E6B8ECD966DBA
GO

Sequence cautioni

The sequence CAL17395.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286690 Genomic DNA. Translation: CAL17395.1. Different initiation.
RefSeqiWP_011589226.1. NC_008260.1.
YP_693667.1. NC_008260.1.

Genome annotation databases

EnsemblBacteriaiCAL17395; CAL17395; ABO_1947.
KEGGiabo:ABO_1947.
PATRICi20841731. VBIAlcBor124741_2039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286690 Genomic DNA. Translation: CAL17395.1. Different initiation.
RefSeqiWP_011589226.1. NC_008260.1.
YP_693667.1. NC_008260.1.

3D structure databases

ProteinModelPortaliQ0VN53.
SMRiQ0VN53. Positions 228-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi393595.ABO_1947.

Proteomic databases

PRIDEiQ0VN53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL17395; CAL17395; ABO_1947.
KEGGiabo:ABO_1947.
PATRICi20841731. VBIAlcBor124741_2039.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciABOR393595:GHRI-1991-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2.

Entry informationi

Entry nameiSYL_ALCBS
AccessioniPrimary (citable) accession number: Q0VN53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 24, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.