Reviewed, UniProtKB/Swiss-Prot Q0VMY5 (PUR9_ALCBS)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	ABO_2015

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000057892

Sequences

	Sequence		Length	Mass (Da)
	Q0VMY5-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 4C40FF4E3C0E576D	FASTA	525	56,122
	10 20 30 40 50 60 MSNVERALIS VSDKTGIVEF AQALADKGVE LLSTGGTFKK LQEAGIAVRE VSDYTGFPEM 70 80 90 100 110 120 MAGRVKTLHP KVHGGILGRR GQDEQVMADN DISPIDLVVV NLYPFEATVA NPDCSLEDAI 130 140 150 160 170 180 ENIDIGGPTM VRSAAKNNAS VGIVTDAADY QRVLDDMAAN NGALSDDLRF DLAIKAFEHT 190 200 210 220 230 240 AAYDSAIANY FGKKVEGQDG MSNLDFPRTI NLNFEKTQNM RYGENPHQKA AFYTERNPAP 250 260 270 280 290 300 ASIATAKQLQ GKELSYNNIA DTDAALECVK GFTKPACVIV KHANPCGVAV SLDGITTAYD 310 320 330 340 350 360 LAFATDTESA FGGIIAFNRK LDAATAQAIV DRQFVEVIIA PSATEEALAI TAAKKNVRVL 370 380 390 400 410 420 VCGEMEGVAA SGLDYKRVNG GLLVQDRDLG MITEGELKVV TKRAPTEAEM HDLIFAWKVA 430 440 450 460 470 480 KFVKSNAIVY AKDRQTVGVG AGQMSRVNSA RIAAIKAEHA GLQVKGSVMA SDAFFPFRDG 490 500 510 520 IDNAAKVGIS CVIQPGGSIR DEEVIAAADE AGMAMVFTGM RHFRH

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL17463.1.
RefSeq	YP_693735.1.
3D structure databases
SMR	Q0VMY5. Positions 4-525.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VMY5.
Genome annotation databases
GeneID	4211146.
GenomeReviews	Gene locus ABO_2015 in contig AM286690_GR.
KEGG	abo:ABO_2015.
NMPDR	fig|393595.12.peg.2017.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HBG498048.
OMA	VVKHVKS.
PhylomeDB	Q0VMY5.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_2015-MONOMER.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_ALCBS

Accession

Primary (citable) accession number: Q0VMY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)