ID   SYC_ALCBS               Reviewed;         460 AA.
AC   Q0VML5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 24.
DE   RecName: Full=Cysteinyl-tRNA synthetase;
DE            EC=6.1.1.16;
DE   AltName: Full=Cysteine--tRNA ligase;
DE            Short=CysRS;
GN   Name=cysS; OrderedLocusNames=ABO_2135;
OS   Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC   Alcanivoracaceae; Alcanivorax.
OX   NCBI_TaxID=393595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16878126; DOI=10.1038/nbt1232;
RA   Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T.,
RA   Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M.,
RA   Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N.,
RA   Lang S., Linke B., McHardy A.C., Meyer F., Nechitaylo T., Puehler A.,
RA   Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M.,
RA   Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N.;
RT   "Genome sequence of the ubiquitous hydrocarbon-degrading marine
RT   bacterium Alcanivorax borkumensis.";
RL   Nat. Biotechnol. 24:997-1004(2006).
CC   -!- CATALYTIC ACTIVITY: ATP + L-cysteine + tRNA(Cys) = AMP +
CC       diphosphate + L-cysteinyl-tRNA(Cys).
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; AM286690; CAL17583.1; -; Genomic_DNA.
DR   RefSeq; YP_693855.1; -.
DR   GeneID; 4212582; -.
DR   GenomeReviews; AM286690_GR; ABO_2135.
DR   KEGG; abo:ABO_2135; -.
DR   NMPDR; fig|393595.12.peg.2133; -.
DR   HOGENOM; Q0VML5; -.
DR   OMA; Q0VML5; NFVTIHD.
DR   BioCyc; ABOR393595:ABO_2135-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:HAMAP.
DR   HAMAP; MF_00041; -; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR015804; Cys-tRNA-synt_Ia_C.
DR   InterPro; IPR015273; Cys-tRNA-synt_Ia_DALR.
DR   InterPro; IPR015803; Cys-tRNA-synt_Ia_N.
DR   InterPro; IPR002308; Cys-tRNA-synth_1a.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR10890; Cys_tRNA-synt_1a; 1.
DR   Pfam; PF09190; DALR_2; 1.
DR   Pfam; PF01406; tRNA-synt_1e; 1.
DR   PRINTS; PR00983; TRNASYNTHCYS.
DR   TIGRFAMs; TIGR00435; cysS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; FALSE_NEG.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Zinc.
FT   CHAIN         1    460       Cysteinyl-tRNA synthetase.
FT                                /FTId=PRO_1000006560.
FT   MOTIF        31     41       "HIGH" region.
FT   MOTIF       266    270       "KMSKS" region.
FT   METAL        29     29       Zinc (By similarity).
FT   METAL       209    209       Zinc (By similarity).
FT   METAL       234    234       Zinc (By similarity).
FT   METAL       238    238       Zinc (By similarity).
FT   BINDING     269    269       ATP (By similarity).
SQ   SEQUENCE   460 AA;  51328 MW;  156608ACA20EEFC3 CRC64;
     MTLRLQNTLT GKKDDFIPLD PNRITLYVCG PTVYNFVHIG NARPVVVFDV LYRLLQRLYP
     NVVYARNITD IDDKIIKAAA DNGEAISTLT ERFTQAFADD MASLNAKTPS IIPKATDHIP
     EMIQMIAILV EKGHAYEADG QVLFAVESMP DYGKLSGRKL EDMLAGARVE VADYKRHPGD
     FILWKPSTDE QPGWDSPWGR GRPGWHIECS AMIHKHLGDV IDIHGGGQDL IFPHHENEIA
     QGCCAHGTDY VRYWLHNGYI NIDGEKMSKS LGNFRLVRDL LTQYHGETLR FALLSSHYRS
     PLNFSADVLD NAEKGLDTLY YALLGRGETV APEAGYQLPD NHPVLDALKD DLNTSEAVSA
     LHAIAGELNK AELDDKPKLK AELLAAADLL GLLTVEPTAW FQNKNVSEDG LSNDAIDALV
     EERTQAKKDK NFARADEIRQ QLTKAGIQLE DTREGTRWSR
//