Reviewed, UniProtKB/Swiss-Prot Q0VMI4 (DXS_ALCBS)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	ABO_2166

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256369

Sequences

	Sequence		Length	Mass (Da)
	Q0VMI4-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6C876D7125DB0A3E	FASTA	645	70,353
	10 20 30 40 50 60 MPKTFTQIPL TRPNTPLLDT LASPADLRKL PTPQLERVVD ELREYLLYAV GQCGGHFGAG 70 80 90 100 110 120 LGVVELTVAL HYLYHTPDDK LVWDVGHQCY PHKILTGRRE SLTSIRQAGG LSGFPKRSES 130 140 150 160 170 180 EYDTFGVGHS STSISAALGM ALGAEMAGSD RRAVAIIGDG AMTAGQAFEA MSHAAHTRSN 190 200 210 220 230 240 LLVILNDNNM SISHNVGGLS NYFARIWASK SYIALREGGK KVLSTMPAAW DFIRRTEESM 250 260 270 280 290 300 KNMVSPDMLF EAIGFNYIGP IDGHNVNELV STLRNLRDLE GPQLLHVYTT KGKGFAPAEA 310 320 330 340 350 360 DPVGYHAINK IEPKPKVQVA VPSKPSAAKQ KLPKYQDIFG QWLCDMAEQD PRLVGITPAM 370 380 390 400 410 420 CEGSGMVEFS RRFPGRYHDV AICEQHAVTL AGGLACENQK PVVAIYSTFL QRGYDQLIHD 430 440 450 460 470 480 VALQELDVTF GLDRAGLVGE DGATHGGVFD LAYLRTVPNM IIAAPSDENE CRQLLYSAYQ 490 500 510 520 530 540 HEGPAAVRYP RGTGPGATIE QTMTALPIGQ SRTLREGLQV AILAFGAMVP AALEAAIPLN 550 560 570 580 590 600 ATVIDMRWVK PLDRDAILRA AAQHTLLVTV EDHQQMGGAG SAVNELLHEE AVVLDVLNLA 610 620 630 640 LPDHFIHHGK RDVLLAQAGL DAAGIERQIR ERLNRQQRLR EAHQR

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL17614.1.
RefSeq	YP_693886.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VMI4.
Genome annotation databases
GeneID	4213338.
GenomeReviews	Gene locus ABO_2166 in contig AM286690_GR.
KEGG	abo:ABO_2166.
NMPDR	fig|393595.12.peg.2166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VMI4.
OMA	QRFPDRY.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_2166-MON.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. False negative. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ALCBS

Accession

Primary (citable) accession number: Q0VMI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)