Reviewed, UniProtKB/Swiss-Prot Q0VMG4 (ALR_ALCBS)

Names and origin

Protein names

Recommended name:
    Alanine racemase
    EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	ABO_2186

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Alanine racemase HAMAP MF_01201		PRO_1000065968
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	251	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VMG4-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 7CBBC19C326EAA06	FASTA	353	37,526
	10 20 30 40 50 60 MRGTRVEIRL AALRNNALRA AELAGDAQVF AMVKANGYGH GLLLAAETML DSVSGLGVAV 70 80 90 100 110 120 LDEARTLREH GIALPILVAE GFFDAEELEA AARLSLEVVV HSLWQVELLL ANPCPVRIWL 130 140 150 160 170 180 KVNAGMNRLG LRPNEALSAA ARLSQAGNAP VGVMSHFACA DMDEDIHSEK QLLLAGSVAE 190 200 210 220 230 240 QLQLPLSASN SAALLRYPRA HAQRVRPGIM LYGSSPFNWQ TAAELGLQVS HRFSARLIAI 250 260 270 280 290 300 NAVEAGESVG YGATWTASDP RQIGVVAVGY GDGYPRHAPS GTPVAVNGVV TTLVGRVSMD 310 320 330 340 350 MITIDVTGLS ASVGDEVELW GDVVDVDDVA RACGTISYEL FCQITQRPER TIV

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286690 Genomic DNA. Translation: CAL17634.1.
RefSeq	YP_693906.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4213284.
GenomeReviews	Gene locus ABO_2186 in contig AM286690_GR.
KEGG	abo:ABO_2186.
NMPDR	fig|393595.12.peg.2186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0VMG4.
OMA	Q0VMG4. TGLKPAM.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_2186-MON.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_ALCBS

Accession

Primary (citable) accession number: Q0VMG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)