Q0VME6 (MIAA_ALCBS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA dimethylallyltransferase
EC=2.5.1.75

Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name=DMAPP:tRNA dimethylallyltransferase
Short name=DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name=IPP transferase
Short name=IPPT
Short name=IPTase

Gene names

Name:	miaA
Ordered Locus Names:	ABO_2204

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity. HAMAP MF_00185
Catalytic activity	Dimethylallyl diphosphate + tRNA = diphosphate + tRNA containing 6-dimethylallyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity. HAMAP MF_00185
Subunit structure	Monomer By similarity. HAMAP MF_00185
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA dimethylallyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	tRNA dimethylallyltransferase HAMAP MF_00185		PRO_1000020559
Regions
	Nucleotide binding	14 – 21	8	ATP Potential
	Region	16 – 21	6	Substrate binding By similarity
	Region	39 – 42	4	Interaction with substrate tRNA By similarity
	Region	163 – 167	5	Interaction with substrate tRNA By similarity
Sites
	Site	105	1	Interaction with substrate tRNA By similarity
	Site	127	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VME6 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 8078F24D5BF339C2	FASTA	329	36,537
	10 20 30 40 50 60 MMPEASLPIL LLMGPTCSGK TALAIEAAQT LPIEIINVDS ALVYKGLNIG AARPTEEELA 70 80 90 100 110 120 LAPHRLLGFR DLTEPYSAAD FRADALKHIE QIHNNGKLPL LVGGTVLYFK ALVEGLAPLP 130 140 150 160 170 180 EADPVVRAEI AALADAQGWP AVHQALAEVD PVTAARLKPT DKQRLQRALE VFRLTGRPLA 190 200 210 220 230 240 AFHAEHDKTL TKSRDGLSQF SGLPYPVLST ALAPQDRAWL HEQIALRFRA MLNSGLQEEV 250 260 270 280 290 300 EALRQQPQLN ADLPGIRAVG YRQMWEYLDG MYGYDSMVER GIIATRQLAK RQFTWLRKWP 310 320 ELQWFDSNDR SQRDALFGLL AKNCKSVFS

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SK2 / ATCC 700651 / DSM 11573.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL17652.1.
RefSeq	YP_693924.1. NC_008260.1.
3D structure databases
ProteinModelPortal	Q0VME6.
SMR	Q0VME6. Positions 200-307.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VME6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4211582.
GenomeReviews	Gene locus ABO_2204 in contig AM286690_GR.
KEGG	abo:ABO_2204.
NMPDR	fig|393595.12.peg.2203.
PATRIC	20842277. VBIAlcBor124741_2307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0324.
HOGENOM	HBG559459.
OMA	HTRNYAK.
PhylomeDB	Q0VME6.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_2204-MONOMER.
Family and domain databases
HAMAP	MF_00185. IPP_trans. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
KO	K00791.
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00174. MiaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_ALCBS

Accession

Primary (citable) accession number: Q0VME6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program