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Protein

Biotin synthase

Gene

bioB

Organism
Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi71Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi74Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi111Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi142Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi202Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi274Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciABOR393595:G1G1O-2289-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:ABO_2220
OrganismiAlcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)
Taxonomic identifieri393595 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesAlcanivoracaceaeAlcanivorax
Proteomesi
  • UP000008871 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003811901 – 383Biotin synthaseAdd BLAST383

Proteomic databases

PRIDEiQ0VMD0

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi393595.ABO_2220

Structurei

3D structure databases

ProteinModelPortaliQ0VMD0
SMRiQ0VMD0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q0VMD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAIASSPQS RSTDMAVRHD WKRSEIDALF ALPFNDLLFQ AAALHRAHFD
60 70 80 90 100
PNAVQVSTLL SIKTGACPED CKYCSQSGHY NTELEKEKLL EVSRVVEEAR
110 120 130 140 150
AARQNGASRF CMGAAWRSPR DKDMPYVLDM IRQVKDLGME TCMTLGMLNE
160 170 180 190 200
GQAGALAEAG LDYYNHNLDT SPEYYGQVIT TRTYNDRLST LANVRDAGMK
210 220 230 240 250
VCCGGIVGMG EERNDRVGLL QQLANLPHHP ESVPINMLVK IEGTPLADVD
260 270 280 290 300
DLDPFEFVRT VAVARILMPQ SYVRLSAGRQ EMNDEAQALC FMAGANSIFY
310 320 330 340 350
GERLLTTDNP EANHDQRLFK RLGIHPLDPR HEASDEAHEE AIQAQVSEQQ
360 370 380
AEEAGLFYNA MDSKRTAKHV LDKDTGRPTA TEH
Length:383
Mass (Da):42,613
Last modified:September 5, 2006 - v1
Checksum:i405C0DAA59FE4031
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286690 Genomic DNA Translation: CAL17668.1
RefSeqiWP_011589496.1, NC_008260.1

Genome annotation databases

EnsemblBacteriaiCAL17668; CAL17668; ABO_2220
KEGGiabo:ABO_2220

Similar proteinsi

Entry informationi

Entry nameiBIOB_ALCBS
AccessioniPrimary (citable) accession number: Q0VMD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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