Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q0VMD0 (BIOB_ALCBS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:ABO_2220
OrganismAlcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]
Taxonomic identifier393595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesAlcanivoracaceaeAlcanivorax

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383Biotin synthase HAMAP-Rule MF_01694
PRO_0000381190

Sites

Metal binding671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1111Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1421Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2021Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2741Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0VMD0 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 405C0DAA59FE4031

FASTA38342,613
        10         20         30         40         50         60 
MPAIASSPQS RSTDMAVRHD WKRSEIDALF ALPFNDLLFQ AAALHRAHFD PNAVQVSTLL 

        70         80         90        100        110        120 
SIKTGACPED CKYCSQSGHY NTELEKEKLL EVSRVVEEAR AARQNGASRF CMGAAWRSPR 

       130        140        150        160        170        180 
DKDMPYVLDM IRQVKDLGME TCMTLGMLNE GQAGALAEAG LDYYNHNLDT SPEYYGQVIT 

       190        200        210        220        230        240 
TRTYNDRLST LANVRDAGMK VCCGGIVGMG EERNDRVGLL QQLANLPHHP ESVPINMLVK 

       250        260        270        280        290        300 
IEGTPLADVD DLDPFEFVRT VAVARILMPQ SYVRLSAGRQ EMNDEAQALC FMAGANSIFY 

       310        320        330        340        350        360 
GERLLTTDNP EANHDQRLFK RLGIHPLDPR HEASDEAHEE AIQAQVSEQQ AEEAGLFYNA 

       370        380 
MDSKRTAKHV LDKDTGRPTA TEH 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM286690 Genomic DNA. Translation: CAL17668.1.
RefSeqYP_693940.1. NC_008260.1.

3D structure databases

ProteinModelPortalQ0VMD0.
SMRQ0VMD0. Positions 18-326.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING393595.ABO_2220.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL17668; CAL17668; ABO_2220.
GeneID4212134.
KEGGabo:ABO_2220.
PATRIC20842309. VBIAlcBor124741_2323.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycABOR393595:GHRI-2269-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ALCBS
AccessionPrimary (citable) accession number: Q0VMD0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways