Reviewed, UniProtKB/Swiss-Prot Q0VM06 (TYSY_ALCBS)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	ABO_2344

Organism

Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) [Complete proteome] [HAMAP]

Taxonomic identifier

393595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Alcanivoracaceae › Alcanivorax

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity. HAMAP MF_00008
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Thymidylate synthase HAMAP MF_00008		PRO_1000000571
Sites
	Active site	159	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VM06-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 7807D5D137C0D0A3	FASTA	277	31,049
	10 20 30 40 50 60 MKQYLDLLRH VREHGTYKED RTGTGTYAVF GYQMRYDLSQ GFPMLTTKKL HLRSIIHELL 70 80 90 100 110 120 WFLSGDTNIR YLKENGVSIW DGWATPEGEL GPVYGEQWRS WKTADGGVID QITEVLKEIK 130 140 150 160 170 180 RNPDSRRLVV SAWNPAVLPD PSISPDANAA AGKQALPPCH CLFQFYVADG KLSCQLYQRS 190 200 210 220 230 240 GDIFLGVPFN IASYALLTLM MAQVCDLQPG DFVHTLGDAH LYSNHLEQAD TQLARTPGPL 250 260 270 PTMTLNPAVK DLFAFTFDDF TVSNYNPDAH IKAPVAI

References

[1]

"Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis." Schneiker S., Martins dos Santos V.A.P., Bartels D., Bekel T., Brecht M., Buhrmester J., Chernikova T.N., Denaro R., Ferrer M., Gertler C., Goesmann A., Golyshina O.V., Kaminski F., Khachane A.N., Lang S., Linke B., McHardy A.C., Meyer F. , Nechitaylo T., Puehler A., Regenhardt D., Rupp O., Sabirova J.S., Selbitschka W., Yakimov M.M., Timmis K.N., Vorhoelter F.-J., Weidner S., Kaiser O., Golyshin P.N. Nat. Biotechnol. 24:997-1004(2006) [PubMed: 16878126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286690 Genomic DNA. Translation: CAL17792.1.
RefSeq	YP_694064.1.
3D structure databases
SMR	Q0VM06. Positions 1-277.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VM06.
Genome annotation databases
GeneID	4211942.
GenomeReviews	Gene locus ABO_2344 in contig AM286690_GR.
KEGG	abo:ABO_2344.
NMPDR	fig|393595.12.peg.2345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HBG588098.
OMA	RMALAPC.
PhylomeDB	Q0VM06.
Enzyme and pathway databases
BioCyc	ABOR393595:ABO_2344-MONOMER.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR000398. Thymidylate_synthase. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_ALCBS

Accession

Primary (citable) accession number: Q0VM06

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)