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Q0VGT2

- GLI2_MOUSE

UniProt

Q0VGT2 - GLI2_MOUSE

Protein

Zinc finger protein GLI2

Gene

Gli2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element. Is involved in the smoothened (SHH) signaling pathway.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri417 – 44428C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri455 – 47723C2H2-type 2; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri483 – 50725C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri513 – 53826C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri544 – 56926C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: MGI
    6. transcription regulatory region DNA binding Source: MGI
    7. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. anatomical structure development Source: MGI
    2. anatomical structure formation involved in morphogenesis Source: MGI
    3. axon guidance Source: MGI
    4. branching morphogenesis of an epithelial tube Source: MGI
    5. cell differentiation Source: MGI
    6. cell proliferation Source: MGI
    7. cellular response to organic cyclic compound Source: MGI
    8. cerebellar cortex morphogenesis Source: MGI
    9. chondrocyte differentiation Source: MGI
    10. cochlea morphogenesis Source: MGI
    11. developmental growth Source: MGI
    12. dorsal/ventral neural tube patterning Source: MGI
    13. dorsal/ventral pattern formation Source: MGI
    14. embryonic digestive tract development Source: MGI
    15. embryonic digit morphogenesis Source: MGI
    16. epidermal cell differentiation Source: Ensembl
    17. floor plate formation Source: MGI
    18. head development Source: MGI
    19. heart development Source: MGI
    20. hindbrain development Source: MGI
    21. hindgut morphogenesis Source: MGI
    22. in utero embryonic development Source: MGI
    23. kidney development Source: MGI
    24. lung development Source: MGI
    25. mammary gland development Source: MGI
    26. mammary gland duct morphogenesis Source: MGI
    27. morphogenesis of an epithelium Source: MGI
    28. negative regulation of chondrocyte differentiation Source: MGI
    29. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    30. neural tube development Source: MGI
    31. neuron development Source: MGI
    32. notochord regression Source: MGI
    33. odontogenesis of dentin-containing tooth Source: MGI
    34. osteoblast development Source: MGI
    35. osteoblast differentiation Source: MGI
    36. pattern specification process Source: MGI
    37. pituitary gland development Source: MGI
    38. positive regulation of cell proliferation Source: MGI
    39. positive regulation of DNA replication Source: Ensembl
    40. positive regulation of neuron differentiation Source: MGI
    41. positive regulation of transcription, DNA-templated Source: UniProtKB
    42. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    43. prostatic bud formation Source: MGI
    44. proximal/distal pattern formation Source: MGI
    45. regulation of smoothened signaling pathway Source: MGI
    46. response to mechanical stimulus Source: Ensembl
    47. skeletal system development Source: MGI
    48. smoothened signaling pathway Source: MGI
    49. smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: MGI
    50. smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation Source: MGI
    51. smoothened signaling pathway involved in spinal cord motor neuron cell fate specification Source: MGI
    52. smoothened signaling pathway involved in ventral spinal cord interneuron specification Source: MGI
    53. spinal cord dorsal/ventral patterning Source: MGI
    54. spinal cord motor neuron differentiation Source: MGI
    55. spinal cord ventral commissure morphogenesis Source: MGI
    56. transcription, DNA-templated Source: UniProtKB-KW
    57. tube development Source: MGI
    58. ventral midline development Source: MGI
    59. ventral spinal cord development Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein GLI2
    Alternative name(s):
    Tax helper protein
    Gene namesi
    Name:Gli2
    Synonyms:Thp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:95728. Gli2.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus.

    GO - Cellular componenti

    1. ciliary tip Source: MGI
    2. cilium Source: MGI
    3. cytoplasm Source: MGI
    4. membrane Source: MGI
    5. motile primary cilium Source: MGI
    6. nuclear speck Source: MGI
    7. nucleus Source: UniProtKB
    8. primary cilium Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15441544Zinc finger protein GLI2PRO_0000406215Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei145 – 1451PhosphoserineBy similarity
    Modified residuei230 – 2301PhosphoserineBy similarity
    Modified residuei232 – 2321PhosphoserineBy similarity
    Modified residuei238 – 2381PhosphoserineBy similarity
    Modified residuei385 – 3851Phosphoserine; by DYRK2By similarity
    Modified residuei707 – 7071Phosphoserine1 Publication
    Modified residuei708 – 7081Phosphothreonine1 Publication
    Modified residuei740 – 7401N6-acetyllysine; by EP300By similarity
    Modified residuei997 – 9971Phosphoserine; by DYRK2By similarity

    Post-translational modificationi

    Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2; this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2 By similarity.By similarity
    Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ0VGT2.

    PTM databases

    PhosphoSiteiQ0VGT2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ0VGT2.
    BgeeiQ0VGT2.
    GenevestigatoriQ0VGT2.

    Interactioni

    Subunit structurei

    Interaction with ZIC1 By similarity. Interacts with SUFU.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SUFUQ9UMX13EBI-9344284,EBI-740595From a different organism.

    Protein-protein interaction databases

    BioGridi199943. 13 interactions.
    DIPiDIP-60772N.
    IntActiQ0VGT2. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VGT2.
    SMRiQ0VGT2. Positions 416-570.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi789 – 85466Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri417 – 44428C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri455 – 47723C2H2-type 2; degeneratePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri483 – 50725C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri513 – 53826C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri544 – 56926C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00740000115106.
    HOGENOMiHOG000290688.
    HOVERGENiHBG005844.
    InParanoidiQ0VGT2.
    KOiK16798.
    OMAiSYPQQSH.
    OrthoDBiEOG7X6KZ8.
    TreeFamiTF350216.

    Family and domain databases

    Gene3Di3.30.160.60. 5 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00096. zf-C2H2. 2 hits.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 5 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0VGT2-1 [UniParc]FASTAAdd to Basket

    « Hide

    METSAPAPAL EKKEAKSGLL EDSSFPDPGK KACPLAVAAA VAAHGVPQQL     50
    LPAFHAPLPI DMRHQEGRYH YDPHSVHSVH GPPTLSGSPV ISDISLIRLS 100
    PHPAGPGESP FSAHHPYVNP HMEHYLRSVH SSPTLSMISA ARGLSPADVA 150
    HEHLKERGLF SLAAPGTNPS DYYHQMTLMA SHPTPYGDLL MQSGGAASAP 200
    HLHDYLNPVD ASRFSSPRVT PRLSRKRALS ISPLSDASLD LQRMIRTSPN 250
    SLVAYINNSR SSSAASGSYG HLSAGALSPA FTFPHPINPV AYQQILSQQR 300
    GLGSAFGHTP PLIQPSPTFL AQQPMTLTSI STMPTQLSSS SSNCLNDANQ 350
    NKQNSESAVS STVNPITIHK RSKVKTEAEG LRPASPLGLT QEQLADLKED 400
    LDRDDCKQEA EVVIYETNCH WADCTKEYDT QEQLVHHINN EHIHGEKKEF 450
    VCRWQACTRE QKPFKAQYML VVHMRRHTGE KPHKCTFEGC SKAYSRLENL 500
    KTHLRSHTGE KPYVCEHEGC NKAFSNASDR AKHQNRTHSN EKPYICKIPG 550
    CTKRYTDPSS LRKHVKTVHG PDAHVTKKQR NDVHVRAPLL KENGDNEASA 600
    EPGGRGPEES VEASSTSHTV EDCLHIKAIK TESSGLCQSS PGAQSSCSSE 650
    PSPLGSAPNN DSGMEMPGTG PGSLGDLTAL ADTCPGADTS ALAAPSTGGL 700
    QLRKHMSTVH RFEQLKREKL KSLKDSCSWA GPAPHTRNTK LPPLPVNGSV 750
    LENFNNTGGG GPAGLLPSQR LPELTEVTML SQLQERRDSS TSTMSSAYTV 800
    SRRSSGISPY FSSRRSSEAS PLGGLRPHNA SSADSYDPIS TDASRRSSEA 850
    SQCSGGGPGL LNLTPAQQYN LRAKYAAATG GPPPTPLPGL DRVSLRTRLA 900
    LLDAPERALP GACPHPLGPR RGSDGPTYSH GHGHGYAGAA PAFPHEGPNS 950
    STRRASDPVR RPDPLILPRV QRFHSTHNMN PGSLPPCADR RGLHVQSHPS 1000
    VDSNLTRNAY SPRPPSINEN VVMEAVAAGV DGPGLECDLG LVEDELVLPD 1050
    DVVQYIKAHT GGTLDDGIRQ GYPTEGTGFP ENSKLPSPGL QGHRRLAAAD 1100
    SNMGPSAPGL GGCQLSYSPS SNLNKSNMPV QWNEVSSGTV DALPTQVKPP 1150
    PFPHSNLAVV QQKPAFGQYP GYNPQSVQSS SGGLDSTQPH LQLRGAPSAS 1200
    RGSYTQQPRQ PAAGSQCLGM SAAMSPQASY SQAHPQLSPN IVSGSLNQFS 1250
    PSCSNMAAKP SHLGLPQQME VVPNATIMNG HQREHGVPNS SLAAVSQPHP 1300
    VLSYPQQDSY QQGSNLLSSH QPGFMESQQN AGFGLMQPRP PLEPNTASRH 1350
    RGVRSGQQQL YARTTGQAMV TSANQETAEA MPKGPAGTMV SLAPQPSQDT 1400
    GRAQDQNTLY YYGQIHMYEQ NGGCPAVQPQ PPQPQACSDS IQPEPLPSPG 1450
    VNQVSSTVDS QLLEPPQIDF DAIMDDGDHS SLFSGALSPT LLHNLSQNSS 1500
    RLTTPRNSLT LPSIPAGISN MAVGDMSSML TSLAEESKFL NMMT 1544
    Length:1,544
    Mass (Da):165,031
    Last modified:July 27, 2011 - v2
    Checksum:iEA290773B3DE1D6A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711Y → S in AAH85190. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC109271 Genomic DNA. No translation available.
    AC122287 Genomic DNA. No translation available.
    AC132589 Genomic DNA. No translation available.
    BC085190 mRNA. Translation: AAH85190.1.
    CCDSiCCDS35692.1.
    RefSeqiNP_001074594.1. NM_001081125.1.
    XP_006529203.1. XM_006529140.1.
    UniGeneiMm.273292.

    Genome annotation databases

    EnsembliENSMUST00000062483; ENSMUSP00000054837; ENSMUSG00000048402.
    GeneIDi14633.
    KEGGimmu:14633.
    UCSCiuc007cim.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC109271 Genomic DNA. No translation available.
    AC122287 Genomic DNA. No translation available.
    AC132589 Genomic DNA. No translation available.
    BC085190 mRNA. Translation: AAH85190.1 .
    CCDSi CCDS35692.1.
    RefSeqi NP_001074594.1. NM_001081125.1.
    XP_006529203.1. XM_006529140.1.
    UniGenei Mm.273292.

    3D structure databases

    ProteinModelPortali Q0VGT2.
    SMRi Q0VGT2. Positions 416-570.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199943. 13 interactions.
    DIPi DIP-60772N.
    IntActi Q0VGT2. 1 interaction.

    PTM databases

    PhosphoSitei Q0VGT2.

    Proteomic databases

    PRIDEi Q0VGT2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000062483 ; ENSMUSP00000054837 ; ENSMUSG00000048402 .
    GeneIDi 14633.
    KEGGi mmu:14633.
    UCSCi uc007cim.1. mouse.

    Organism-specific databases

    CTDi 2736.
    MGIi MGI:95728. Gli2.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00740000115106.
    HOGENOMi HOG000290688.
    HOVERGENi HBG005844.
    InParanoidi Q0VGT2.
    KOi K16798.
    OMAi SYPQQSH.
    OrthoDBi EOG7X6KZ8.
    TreeFami TF350216.

    Miscellaneous databases

    NextBioi 286486.
    PROi Q0VGT2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q0VGT2.
    Bgeei Q0VGT2.
    Genevestigatori Q0VGT2.

    Family and domain databases

    Gene3Di 3.30.160.60. 5 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00096. zf-C2H2. 2 hits.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 5 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.
    3. "Physical and functional interactions between Zic and Gli proteins."
      Koyabu Y., Nakata K., Mizugishi K., Aruga J., Mikoshiba K.
      J. Biol. Chem. 276:6889-6892(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZIC1, SUBCELLULAR LOCATION.
    4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-707 AND THR-708, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    5. "Kif7 regulates Gli2 through Sufu-dependent and -independent functions during skin development and tumorigenesis."
      Li Z.J., Nieuwenhuis E., Nien W., Zhang X., Zhang J., Puviindran V., Wainwright B.J., Kim P.C., Hui C.C.
      Development 139:4152-4161(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SUFU, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiGLI2_MOUSE
    AccessioniPrimary (citable) accession number: Q0VGT2
    Secondary accession number(s): E9PYJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 5, 2011
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3