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Q0VGT2 (GLI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein GLI2
Alternative name(s):
Tax helper protein
Gene names
Name:Gli2
Synonyms:Thp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1544 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element. Is involved in the smoothened (SHH) signaling pathway.

Subunit structure

Interaction with ZIC1 By similarity.

Subcellular location

Nucleus Ref.3.

Post-translational modification

Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2; this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2 By similarity.

Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy By similarity.

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanatomical structure development

Inferred from mutant phenotype PubMed 11485934. Source: MGI

anatomical structure formation involved in morphogenesis

Inferred from genetic interaction PubMed 9731531. Source: MGI

axon guidance

Inferred from mutant phenotype PubMed 10409510. Source: MGI

branching morphogenesis of an epithelial tube

Inferred from mutant phenotype PubMed 9731531. Source: MGI

cell differentiation

Inferred from mutant phenotype PubMed 9636069PubMed 9655799. Source: MGI

cell proliferation

Inferred from genetic interaction PubMed 19056373. Source: MGI

cellular response to organic cyclic compound

Inferred from direct assay PubMed 19103752. Source: MGI

cerebellar cortex morphogenesis

Inferred from mutant phenotype PubMed 16571625. Source: MGI

chondrocyte differentiation

Inferred from genetic interaction PubMed 23333501. Source: MGI

cochlea morphogenesis

Inferred from mutant phenotype PubMed 17395647. Source: MGI

developmental growth

Inferred from mutant phenotype PubMed 16571630. Source: MGI

dorsal/ventral neural tube patterning

Inferred from direct assay PubMed 9247260. Source: MGI

dorsal/ventral pattern formation

Inferred from genetic interaction PubMed 16342201. Source: MGI

embryonic digestive tract development

Inferred from mutant phenotype PubMed 10693670. Source: MGI

embryonic digit morphogenesis

Inferred from mutant phenotype PubMed 19056373. Source: MGI

epidermal cell differentiation

Inferred from electronic annotation. Source: Ensembl

floor plate formation

Inferred from mutant phenotype PubMed 9636069PubMed 9655799. Source: MGI

head development

Inferred from mutant phenotype PubMed 19056373. Source: MGI

heart development

Inferred from genetic interaction PubMed 11172440. Source: MGI

hindbrain development

Inferred from mutant phenotype PubMed 15496441PubMed 8575294. Source: MGI

hindgut morphogenesis

Inferred from mutant phenotype PubMed 11485934. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 19056373. Source: MGI

kidney development

Inferred from genetic interaction PubMed 11172440. Source: MGI

lung development

Inferred from mutant phenotype PubMed 9731531. Source: MGI

mammary gland development

Inferred from mutant phenotype PubMed 11783999. Source: MGI

mammary gland duct morphogenesis

Inferred from mutant phenotype PubMed 11783999. Source: MGI

morphogenesis of an epithelium

Inferred from mutant phenotype PubMed 16707121. Source: MGI

negative regulation of chondrocyte differentiation

Inferred from mutant phenotype PubMed 17328886. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 16611981. Source: MGI

neural tube development

Inferred from mutant phenotype PubMed 19056373. Source: MGI

neuron development

Inferred from mutant phenotype PubMed 16267219. Source: MGI

notochord regression

Inferred from genetic interaction PubMed 10725236. Source: MGI

odontogenesis of dentin-containing tooth

Inferred from mutant phenotype PubMed 9655803. Source: MGI

osteoblast development

Inferred from direct assay PubMed 16880529. Source: MGI

osteoblast differentiation

Inferred from genetic interaction PubMed 22771375. Source: MGI

pattern specification process

Inferred from mutant phenotype PubMed 8575294. Source: MGI

pituitary gland development

Inferred from genetic interaction PubMed 10725236. Source: MGI

positive regulation of DNA replication

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 15496441PubMed 9731531. Source: MGI

positive regulation of neuron differentiation

Inferred from genetic interaction PubMed 16950124. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16611981PubMed 16880529PubMed 16950124. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 17035233. Source: UniProtKB

prostatic bud formation

Inferred from mutant phenotype PubMed 16707121. Source: MGI

proximal/distal pattern formation

Inferred from genetic interaction PubMed 16342201. Source: MGI

regulation of smoothened signaling pathway

Inferred from mutant phenotype PubMed 19056373. Source: MGI

response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

skeletal system development

Inferred from mutant phenotype PubMed 9006072. Source: MGI

smoothened signaling pathway

Inferred from mutant phenotype PubMed 19124651. Source: MGI

smoothened signaling pathway involved in dorsal/ventral neural tube patterning

Inferred from genetic interaction PubMed 17395647PubMed 20159594. Source: MGI

smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation

Inferred from mutant phenotype PubMed 16571625. Source: MGI

smoothened signaling pathway involved in spinal cord motor neuron cell fate specification

Inferred from genetic interaction PubMed 15215207. Source: MGI

smoothened signaling pathway involved in ventral spinal cord interneuron specification

Inferred from genetic interaction PubMed 15215207. Source: MGI

spinal cord dorsal/ventral patterning

Inferred from mutant phenotype PubMed 11748151PubMed 16364285. Source: MGI

spinal cord motor neuron differentiation

Inferred from mutant phenotype PubMed 21552265. Source: MGI

spinal cord ventral commissure morphogenesis

Inferred from mutant phenotype PubMed 10409510. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

tube development

Inferred from genetic interaction PubMed 9731531. Source: MGI

ventral midline development

Inferred from mutant phenotype PubMed 10725236. Source: MGI

ventral spinal cord development

Inferred from mutant phenotype PubMed 11748151. Source: MGI

   Cellular_componentcilium

Inferred from direct assay PubMed 16254602PubMed 19286674PubMed 22689656. Source: MGI

cytoplasm

Inferred from direct assay PubMed 19286674PubMed 19684112. Source: MGI

membrane

Inferred from direct assay PubMed 19286674. Source: MGI

motile primary cilium

Inferred from direct assay PubMed 18590716. Source: MGI

nuclear speck

Inferred from direct assay PubMed 19684112. Source: MGI

nucleus

Inferred from direct assay Ref.3. Source: UniProtKB

primary cilium

Inferred from direct assay PubMed 19654211PubMed 19684112. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15614767PubMed 16880529. Source: MGI

chromatin binding

Inferred from direct assay PubMed 16396903. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 16611981. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 16880529. Source: MGI

transcription regulatory region DNA binding

Inferred from direct assay PubMed 19124651. Source: MGI

zinc ion binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15441544Zinc finger protein GLI2
PRO_0000406215

Regions

Zinc finger417 – 44428C2H2-type 1
Zinc finger455 – 47723C2H2-type 2; degenerate
Zinc finger483 – 50725C2H2-type 3
Zinc finger513 – 53826C2H2-type 4
Zinc finger544 – 56926C2H2-type 5
Compositional bias789 – 85466Ser-rich

Amino acid modifications

Modified residue1451Phosphoserine By similarity
Modified residue2301Phosphoserine By similarity
Modified residue2321Phosphoserine By similarity
Modified residue2381Phosphoserine By similarity
Modified residue3851Phosphoserine; by DYRK2 By similarity
Modified residue7071Phosphoserine Ref.4
Modified residue7081Phosphothreonine Ref.4
Modified residue7401N6-acetyllysine; by EP300 By similarity
Modified residue9971Phosphoserine; by DYRK2 By similarity

Experimental info

Sequence conflict711Y → S in AAH85190. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q0VGT2 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: EA290773B3DE1D6A

FASTA1,544165,031
        10         20         30         40         50         60 
METSAPAPAL EKKEAKSGLL EDSSFPDPGK KACPLAVAAA VAAHGVPQQL LPAFHAPLPI 

        70         80         90        100        110        120 
DMRHQEGRYH YDPHSVHSVH GPPTLSGSPV ISDISLIRLS PHPAGPGESP FSAHHPYVNP 

       130        140        150        160        170        180 
HMEHYLRSVH SSPTLSMISA ARGLSPADVA HEHLKERGLF SLAAPGTNPS DYYHQMTLMA 

       190        200        210        220        230        240 
SHPTPYGDLL MQSGGAASAP HLHDYLNPVD ASRFSSPRVT PRLSRKRALS ISPLSDASLD 

       250        260        270        280        290        300 
LQRMIRTSPN SLVAYINNSR SSSAASGSYG HLSAGALSPA FTFPHPINPV AYQQILSQQR 

       310        320        330        340        350        360 
GLGSAFGHTP PLIQPSPTFL AQQPMTLTSI STMPTQLSSS SSNCLNDANQ NKQNSESAVS 

       370        380        390        400        410        420 
STVNPITIHK RSKVKTEAEG LRPASPLGLT QEQLADLKED LDRDDCKQEA EVVIYETNCH 

       430        440        450        460        470        480 
WADCTKEYDT QEQLVHHINN EHIHGEKKEF VCRWQACTRE QKPFKAQYML VVHMRRHTGE 

       490        500        510        520        530        540 
KPHKCTFEGC SKAYSRLENL KTHLRSHTGE KPYVCEHEGC NKAFSNASDR AKHQNRTHSN 

       550        560        570        580        590        600 
EKPYICKIPG CTKRYTDPSS LRKHVKTVHG PDAHVTKKQR NDVHVRAPLL KENGDNEASA 

       610        620        630        640        650        660 
EPGGRGPEES VEASSTSHTV EDCLHIKAIK TESSGLCQSS PGAQSSCSSE PSPLGSAPNN 

       670        680        690        700        710        720 
DSGMEMPGTG PGSLGDLTAL ADTCPGADTS ALAAPSTGGL QLRKHMSTVH RFEQLKREKL 

       730        740        750        760        770        780 
KSLKDSCSWA GPAPHTRNTK LPPLPVNGSV LENFNNTGGG GPAGLLPSQR LPELTEVTML 

       790        800        810        820        830        840 
SQLQERRDSS TSTMSSAYTV SRRSSGISPY FSSRRSSEAS PLGGLRPHNA SSADSYDPIS 

       850        860        870        880        890        900 
TDASRRSSEA SQCSGGGPGL LNLTPAQQYN LRAKYAAATG GPPPTPLPGL DRVSLRTRLA 

       910        920        930        940        950        960 
LLDAPERALP GACPHPLGPR RGSDGPTYSH GHGHGYAGAA PAFPHEGPNS STRRASDPVR 

       970        980        990       1000       1010       1020 
RPDPLILPRV QRFHSTHNMN PGSLPPCADR RGLHVQSHPS VDSNLTRNAY SPRPPSINEN 

      1030       1040       1050       1060       1070       1080 
VVMEAVAAGV DGPGLECDLG LVEDELVLPD DVVQYIKAHT GGTLDDGIRQ GYPTEGTGFP 

      1090       1100       1110       1120       1130       1140 
ENSKLPSPGL QGHRRLAAAD SNMGPSAPGL GGCQLSYSPS SNLNKSNMPV QWNEVSSGTV 

      1150       1160       1170       1180       1190       1200 
DALPTQVKPP PFPHSNLAVV QQKPAFGQYP GYNPQSVQSS SGGLDSTQPH LQLRGAPSAS 

      1210       1220       1230       1240       1250       1260 
RGSYTQQPRQ PAAGSQCLGM SAAMSPQASY SQAHPQLSPN IVSGSLNQFS PSCSNMAAKP 

      1270       1280       1290       1300       1310       1320 
SHLGLPQQME VVPNATIMNG HQREHGVPNS SLAAVSQPHP VLSYPQQDSY QQGSNLLSSH 

      1330       1340       1350       1360       1370       1380 
QPGFMESQQN AGFGLMQPRP PLEPNTASRH RGVRSGQQQL YARTTGQAMV TSANQETAEA 

      1390       1400       1410       1420       1430       1440 
MPKGPAGTMV SLAPQPSQDT GRAQDQNTLY YYGQIHMYEQ NGGCPAVQPQ PPQPQACSDS 

      1450       1460       1470       1480       1490       1500 
IQPEPLPSPG VNQVSSTVDS QLLEPPQIDF DAIMDDGDHS SLFSGALSPT LLHNLSQNSS 

      1510       1520       1530       1540 
RLTTPRNSLT LPSIPAGISN MAVGDMSSML TSLAEESKFL NMMT 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Eye.
[3]"Physical and functional interactions between Zic and Gli proteins."
Koyabu Y., Nakata K., Mizugishi K., Aruga J., Mikoshiba K.
J. Biol. Chem. 276:6889-6892(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZIC1, SUBCELLULAR LOCATION.
[4]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-707 AND THR-708, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC109271 Genomic DNA. No translation available.
AC122287 Genomic DNA. No translation available.
AC132589 Genomic DNA. No translation available.
BC085190 mRNA. Translation: AAH85190.1.
RefSeqNP_001074594.1. NM_001081125.1.
XP_006529203.1. XM_006529140.1.
UniGeneMm.273292.

3D structure databases

ProteinModelPortalQ0VGT2.
SMRQ0VGT2. Positions 416-570.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199943. 13 interactions.

PTM databases

PhosphoSiteQ0VGT2.

Proteomic databases

PRIDEQ0VGT2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000062483; ENSMUSP00000054837; ENSMUSG00000048402.
GeneID14633.
KEGGmmu:14633.
UCSCuc007cim.1. mouse.

Organism-specific databases

CTD2736.
MGIMGI:95728. Gli2.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00740000115106.
HOGENOMHOG000290688.
HOVERGENHBG005844.
InParanoidQ0VGT2.
KOK16798.
OMASYPQQSH.
OrthoDBEOG7X6KZ8.
TreeFamTF350216.

Gene expression databases

ArrayExpressQ0VGT2.
BgeeQ0VGT2.
GenevestigatorQ0VGT2.

Family and domain databases

Gene3D3.30.160.60. 5 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio286486.
PROQ0VGT2.
SOURCESearch...

Entry information

Entry nameGLI2_MOUSE
AccessionPrimary (citable) accession number: Q0VGT2
Secondary accession number(s): E9PYJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot