Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q0VGT2

- GLI2_MOUSE

UniProt

Q0VGT2 - GLI2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Zinc finger protein GLI2

Gene

Gli2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element. Is involved in the smoothened (SHH) signaling pathway. May play a role during embryogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri417 – 44428C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri455 – 47723C2H2-type 2; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri483 – 50725C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri513 – 53826C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri544 – 56926C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI
  3. sequence-specific DNA binding Source: MGI
  4. sequence-specific DNA binding transcription factor activity Source: MGI
  5. transcription regulatory region DNA binding Source: MGI
  6. zinc ion binding Source: Ensembl

GO - Biological processi

  1. anatomical structure development Source: MGI
  2. anatomical structure formation involved in morphogenesis Source: MGI
  3. axon guidance Source: MGI
  4. branching morphogenesis of an epithelial tube Source: MGI
  5. cell differentiation Source: MGI
  6. cell proliferation Source: MGI
  7. cellular response to organic cyclic compound Source: MGI
  8. cerebellar cortex morphogenesis Source: MGI
  9. chondrocyte differentiation Source: MGI
  10. cochlea morphogenesis Source: MGI
  11. developmental growth Source: MGI
  12. dorsal/ventral neural tube patterning Source: MGI
  13. dorsal/ventral pattern formation Source: MGI
  14. embryonic digestive tract development Source: MGI
  15. embryonic digit morphogenesis Source: MGI
  16. epidermal cell differentiation Source: Ensembl
  17. floor plate formation Source: MGI
  18. head development Source: MGI
  19. heart development Source: MGI
  20. hindbrain development Source: MGI
  21. hindgut morphogenesis Source: MGI
  22. in utero embryonic development Source: MGI
  23. kidney development Source: MGI
  24. lung development Source: MGI
  25. mammary gland development Source: MGI
  26. mammary gland duct morphogenesis Source: MGI
  27. morphogenesis of an epithelium Source: MGI
  28. negative regulation of chondrocyte differentiation Source: MGI
  29. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  30. neural tube development Source: MGI
  31. neuron development Source: MGI
  32. notochord regression Source: MGI
  33. odontogenesis of dentin-containing tooth Source: MGI
  34. osteoblast development Source: MGI
  35. osteoblast differentiation Source: MGI
  36. pattern specification process Source: MGI
  37. pituitary gland development Source: MGI
  38. positive regulation of cell proliferation Source: MGI
  39. positive regulation of DNA replication Source: Ensembl
  40. positive regulation of neuron differentiation Source: MGI
  41. positive regulation of transcription, DNA-templated Source: UniProtKB
  42. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  43. prostatic bud formation Source: MGI
  44. proximal/distal pattern formation Source: MGI
  45. regulation of smoothened signaling pathway Source: MGI
  46. response to mechanical stimulus Source: Ensembl
  47. skeletal system development Source: MGI
  48. smoothened signaling pathway Source: MGI
  49. smoothened signaling pathway involved in dorsal/ventral neural tube patterning Source: MGI
  50. smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation Source: MGI
  51. smoothened signaling pathway involved in spinal cord motor neuron cell fate specification Source: MGI
  52. smoothened signaling pathway involved in ventral spinal cord interneuron specification Source: MGI
  53. spinal cord dorsal/ventral patterning Source: MGI
  54. spinal cord motor neuron differentiation Source: MGI
  55. spinal cord ventral commissure morphogenesis Source: MGI
  56. transcription, DNA-templated Source: UniProtKB-KW
  57. tube development Source: MGI
  58. ventral midline development Source: MGI
  59. ventral spinal cord development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein GLI2Curated
Alternative name(s):
Tax helper proteinBy similarity
Gene namesi
Name:Gli2Imported
Synonyms:ThpBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:95728. Gli2.

Subcellular locationi

Nucleus. Cytoplasm
Note: In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus.

GO - Cellular componenti

  1. ciliary tip Source: MGI
  2. cilium Source: MGI
  3. cytoplasm Source: MGI
  4. membrane Source: MGI
  5. motile primary cilium Source: MGI
  6. nuclear speck Source: MGI
  7. nucleus Source: UniProtKB
  8. primary cilium Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15441544Zinc finger protein GLI2PRO_0000406215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei145 – 1451PhosphoserineBy similarity
Modified residuei230 – 2301PhosphoserineBy similarity
Modified residuei232 – 2321PhosphoserineBy similarity
Modified residuei238 – 2381PhosphoserineBy similarity
Modified residuei385 – 3851Phosphoserine; by DYRK2By similarity
Modified residuei707 – 7071Phosphoserine1 Publication
Modified residuei708 – 7081Phosphothreonine1 Publication
Modified residuei740 – 7401N6-acetyllysine; by EP300By similarity
Modified residuei997 – 9971Phosphoserine; by DYRK2By similarity

Post-translational modificationi

Phosphorylated in vitro by ULK3. Phosphorylated by DYRK2; this inhibits GLI2 transcription factor activity and promotes proteasomal degradation of GLI2.By similarity
Acetylation at Lys-740 inhibits Hh target gene expression, probably by impeding entry into chromatin thus preventing promoter occupancy.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ0VGT2.

PTM databases

PhosphoSiteiQ0VGT2.

Expressioni

Gene expression databases

BgeeiQ0VGT2.
ExpressionAtlasiQ0VGT2. baseline and differential.
GenevestigatoriQ0VGT2.

Interactioni

Subunit structurei

Interaction with ZIC1. Interacts with SUFU.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SUFUQ9UMX13EBI-9344284,EBI-740595From a different organism.

Protein-protein interaction databases

BioGridi199943. 13 interactions.
DIPiDIP-60772N.
IntActiQ0VGT2. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ0VGT2.
SMRiQ0VGT2. Positions 416-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi789 – 85466Ser-richAdd
BLAST

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri417 – 44428C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri455 – 47723C2H2-type 2; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri483 – 50725C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri513 – 53826C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri544 – 56926C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000290688.
HOVERGENiHBG005844.
InParanoidiQ0VGT2.
KOiK16798.
OMAiSYPQQSH.
OrthoDBiEOG7X6KZ8.
TreeFamiTF350216.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF00096. zf-C2H2. 2 hits.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0VGT2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METSAPAPAL EKKEAKSGLL EDSSFPDPGK KACPLAVAAA VAAHGVPQQL
60 70 80 90 100
LPAFHAPLPI DMRHQEGRYH YDPHSVHSVH GPPTLSGSPV ISDISLIRLS
110 120 130 140 150
PHPAGPGESP FSAHHPYVNP HMEHYLRSVH SSPTLSMISA ARGLSPADVA
160 170 180 190 200
HEHLKERGLF SLAAPGTNPS DYYHQMTLMA SHPTPYGDLL MQSGGAASAP
210 220 230 240 250
HLHDYLNPVD ASRFSSPRVT PRLSRKRALS ISPLSDASLD LQRMIRTSPN
260 270 280 290 300
SLVAYINNSR SSSAASGSYG HLSAGALSPA FTFPHPINPV AYQQILSQQR
310 320 330 340 350
GLGSAFGHTP PLIQPSPTFL AQQPMTLTSI STMPTQLSSS SSNCLNDANQ
360 370 380 390 400
NKQNSESAVS STVNPITIHK RSKVKTEAEG LRPASPLGLT QEQLADLKED
410 420 430 440 450
LDRDDCKQEA EVVIYETNCH WADCTKEYDT QEQLVHHINN EHIHGEKKEF
460 470 480 490 500
VCRWQACTRE QKPFKAQYML VVHMRRHTGE KPHKCTFEGC SKAYSRLENL
510 520 530 540 550
KTHLRSHTGE KPYVCEHEGC NKAFSNASDR AKHQNRTHSN EKPYICKIPG
560 570 580 590 600
CTKRYTDPSS LRKHVKTVHG PDAHVTKKQR NDVHVRAPLL KENGDNEASA
610 620 630 640 650
EPGGRGPEES VEASSTSHTV EDCLHIKAIK TESSGLCQSS PGAQSSCSSE
660 670 680 690 700
PSPLGSAPNN DSGMEMPGTG PGSLGDLTAL ADTCPGADTS ALAAPSTGGL
710 720 730 740 750
QLRKHMSTVH RFEQLKREKL KSLKDSCSWA GPAPHTRNTK LPPLPVNGSV
760 770 780 790 800
LENFNNTGGG GPAGLLPSQR LPELTEVTML SQLQERRDSS TSTMSSAYTV
810 820 830 840 850
SRRSSGISPY FSSRRSSEAS PLGGLRPHNA SSADSYDPIS TDASRRSSEA
860 870 880 890 900
SQCSGGGPGL LNLTPAQQYN LRAKYAAATG GPPPTPLPGL DRVSLRTRLA
910 920 930 940 950
LLDAPERALP GACPHPLGPR RGSDGPTYSH GHGHGYAGAA PAFPHEGPNS
960 970 980 990 1000
STRRASDPVR RPDPLILPRV QRFHSTHNMN PGSLPPCADR RGLHVQSHPS
1010 1020 1030 1040 1050
VDSNLTRNAY SPRPPSINEN VVMEAVAAGV DGPGLECDLG LVEDELVLPD
1060 1070 1080 1090 1100
DVVQYIKAHT GGTLDDGIRQ GYPTEGTGFP ENSKLPSPGL QGHRRLAAAD
1110 1120 1130 1140 1150
SNMGPSAPGL GGCQLSYSPS SNLNKSNMPV QWNEVSSGTV DALPTQVKPP
1160 1170 1180 1190 1200
PFPHSNLAVV QQKPAFGQYP GYNPQSVQSS SGGLDSTQPH LQLRGAPSAS
1210 1220 1230 1240 1250
RGSYTQQPRQ PAAGSQCLGM SAAMSPQASY SQAHPQLSPN IVSGSLNQFS
1260 1270 1280 1290 1300
PSCSNMAAKP SHLGLPQQME VVPNATIMNG HQREHGVPNS SLAAVSQPHP
1310 1320 1330 1340 1350
VLSYPQQDSY QQGSNLLSSH QPGFMESQQN AGFGLMQPRP PLEPNTASRH
1360 1370 1380 1390 1400
RGVRSGQQQL YARTTGQAMV TSANQETAEA MPKGPAGTMV SLAPQPSQDT
1410 1420 1430 1440 1450
GRAQDQNTLY YYGQIHMYEQ NGGCPAVQPQ PPQPQACSDS IQPEPLPSPG
1460 1470 1480 1490 1500
VNQVSSTVDS QLLEPPQIDF DAIMDDGDHS SLFSGALSPT LLHNLSQNSS
1510 1520 1530 1540
RLTTPRNSLT LPSIPAGISN MAVGDMSSML TSLAEESKFL NMMT
Length:1,544
Mass (Da):165,031
Last modified:July 27, 2011 - v2
Checksum:iEA290773B3DE1D6A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711Y → S in AAH85190. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC109271 Genomic DNA. No translation available.
AC122287 Genomic DNA. No translation available.
AC132589 Genomic DNA. No translation available.
BC085190 mRNA. Translation: AAH85190.1.
CCDSiCCDS35692.1.
RefSeqiNP_001074594.1. NM_001081125.1.
XP_006529203.1. XM_006529140.1.
UniGeneiMm.273292.

Genome annotation databases

EnsembliENSMUST00000062483; ENSMUSP00000054837; ENSMUSG00000048402.
GeneIDi14633.
KEGGimmu:14633.
UCSCiuc007cim.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC109271 Genomic DNA. No translation available.
AC122287 Genomic DNA. No translation available.
AC132589 Genomic DNA. No translation available.
BC085190 mRNA. Translation: AAH85190.1 .
CCDSi CCDS35692.1.
RefSeqi NP_001074594.1. NM_001081125.1.
XP_006529203.1. XM_006529140.1.
UniGenei Mm.273292.

3D structure databases

ProteinModelPortali Q0VGT2.
SMRi Q0VGT2. Positions 416-570.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199943. 13 interactions.
DIPi DIP-60772N.
IntActi Q0VGT2. 1 interaction.

PTM databases

PhosphoSitei Q0VGT2.

Proteomic databases

PRIDEi Q0VGT2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000062483 ; ENSMUSP00000054837 ; ENSMUSG00000048402 .
GeneIDi 14633.
KEGGi mmu:14633.
UCSCi uc007cim.1. mouse.

Organism-specific databases

CTDi 2736.
MGIi MGI:95728. Gli2.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00760000118771.
HOGENOMi HOG000290688.
HOVERGENi HBG005844.
InParanoidi Q0VGT2.
KOi K16798.
OMAi SYPQQSH.
OrthoDBi EOG7X6KZ8.
TreeFami TF350216.

Miscellaneous databases

NextBioi 286486.
PROi Q0VGT2.
SOURCEi Search...

Gene expression databases

Bgeei Q0VGT2.
ExpressionAtlasi Q0VGT2. baseline and differential.
Genevestigatori Q0VGT2.

Family and domain databases

Gene3Di 3.30.160.60. 5 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
Pfami PF00096. zf-C2H2. 2 hits.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 5 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Eye.
  3. "Physical and functional interactions between Zic and Gli proteins."
    Koyabu Y., Nakata K., Mizugishi K., Aruga J., Mikoshiba K.
    J. Biol. Chem. 276:6889-6892(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZIC1, SUBCELLULAR LOCATION.
  4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-707 AND THR-708, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  5. "Kif7 regulates Gli2 through Sufu-dependent and -independent functions during skin development and tumorigenesis."
    Li Z.J., Nieuwenhuis E., Nien W., Zhang X., Zhang J., Puviindran V., Wainwright B.J., Kim P.C., Hui C.C.
    Development 139:4152-4161(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SUFU, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiGLI2_MOUSE
AccessioniPrimary (citable) accession number: Q0VGT2
Secondary accession number(s): E9PYJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3