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Protein

Gamma-aminobutyric acid receptor-associated protein-like 1

Gene

Gabarapl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1172Cleavage; by ATG4BBy similarity

GO - Molecular functioni

  1. GABA receptor binding Source: GO_Central
  2. microtubule binding Source: GO_Central

GO - Biological processi

  1. autophagic vacuole assembly Source: GO_Central
  2. membrane fusion Source: GO_Central
  3. mitochondrion degradation Source: GO_Central
  4. nucleophagy Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor-associated protein-like 1
Alternative name(s):
GABA(A) receptor-associated protein-like 1
Glandular epithelial cell protein 1
Short name:
GEC-1
Gene namesi
Name:Gabarapl1
Synonyms:Gec1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1596143. Gabarapl1.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Cytoplasmic vesicle membrane By similarity; Lipid-anchor By similarity. Endoplasmic reticulum 1 Publication. Golgi apparatus 1 Publication. Cytoplasmic vesicleautophagosome By similarity

GO - Cellular componenti

  1. autophagic vacuole membrane Source: GO_Central
  2. cell body Source: RGD
  3. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  4. cytosol Source: GO_Central
  5. dendrite cytoplasm Source: RGD
  6. dendrite membrane Source: RGD
  7. endoplasmic reticulum Source: RGD
  8. extrinsic component of membrane Source: GO_Central
  9. Golgi apparatus Source: RGD
  10. microtubule Source: UniProtKB-KW
  11. pre-autophagosomal structure Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Golgi apparatus, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116Gamma-aminobutyric acid receptor-associated protein-like 1PRO_0000342410Add
BLAST
Propeptidei117 – 1171Removed in mature formBy similarityPRO_0000420211

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi116 – 1161Phosphatidylethanolamine amidated glycineCurated

Post-translational modificationi

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes (By similarity).By similarity

Keywords - PTMi

Lipoprotein

Proteomic databases

PRIDEiQ0VGK0.

Expressioni

Tissue specificityi

High expression in liver and brain, lower in lung and heart and very low in kidney and skeletal muscle (at protein level). Present in all brain regions and spinal cord (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ0VGK0.

Interactioni

Subunit structurei

Interacts with GABRG2 and beta-tubulin. Interacts with ATG13, OPRK1, RB1CC1 and ULK1. Interacts with TP53INP1 and TP53INP2. Directly interacts with SQSTM1. Interacts with ATG3, ATG7 and MAP15. Interacts with TECPR2. Interacts with TBC1D5 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ0VGK0. 1 interaction.
STRINGi10116.ENSRNOP00000011942.

Structurei

3D structure databases

ProteinModelPortaliQ0VGK0.
SMRiQ0VGK0. Positions 3-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222Interaction with beta-tubulinBy similarityAdd
BLAST
Regioni36 – 6833Interaction with GABRG2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ATG8 family.Curated

Phylogenomic databases

eggNOGiNOG263746.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ0VGK0.
KOiK08341.
OMAiGNSAENF.
OrthoDBiEOG70KGRK.
PhylomeDBiQ0VGK0.
TreeFamiTF314556.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0VGK0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL
60 70 80 90 100
VPSDLTVGQF YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE
110
EDYFLYVAYS DESVYGK
Length:117
Mass (Da):14,044
Last modified:September 5, 2006 - v1
Checksum:i7F56E345255F564B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105627 mRNA. Translation: AAI05628.1.
RefSeqiNP_001037759.1. NM_001044294.1.
UniGeneiRn.118264.

Genome annotation databases

EnsembliENSRNOT00000011942; ENSRNOP00000011942; ENSRNOG00000008498.
GeneIDi689161.
KEGGirno:689161.
UCSCiRGD:1596143. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105627 mRNA. Translation: AAI05628.1.
RefSeqiNP_001037759.1. NM_001044294.1.
UniGeneiRn.118264.

3D structure databases

ProteinModelPortaliQ0VGK0.
SMRiQ0VGK0. Positions 3-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ0VGK0. 1 interaction.
STRINGi10116.ENSRNOP00000011942.

Proteomic databases

PRIDEiQ0VGK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011942; ENSRNOP00000011942; ENSRNOG00000008498.
GeneIDi689161.
KEGGirno:689161.
UCSCiRGD:1596143. rat.

Organism-specific databases

CTDi23710.
RGDi1596143. Gabarapl1.

Phylogenomic databases

eggNOGiNOG263746.
GeneTreeiENSGT00390000012937.
HOGENOMiHOG000232034.
HOVERGENiHBG051706.
InParanoidiQ0VGK0.
KOiK08341.
OMAiGNSAENF.
OrthoDBiEOG70KGRK.
PhylomeDBiQ0VGK0.
TreeFamiTF314556.

Miscellaneous databases

NextBioi738082.

Gene expression databases

GenevestigatoriQ0VGK0.

Family and domain databases

InterProiIPR004241. Atg8_like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10969. PTHR10969. 1 hit.
PfamiPF02991. Atg8. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  2. Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGBRL1_RAT
AccessioniPrimary (citable) accession number: Q0VGK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: September 5, 2006
Last modified: February 4, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.