ID   GLD2_XENTR              Reviewed;         528 AA.
AC   Q0VFA3; A4IIZ6;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   16-JUN-2009, entry version 21.
DE   RecName: Full=Poly(A) RNA polymerase GLD2;
DE            EC=2.7.7.19;
DE   AltName: Full=PAP-associated domain-containing protein 4;
GN   Name=papd4; Synonyms=gld2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Amphibia; Batrachia; Anura; Mesobatrachia; Pipoidea; Pipidae;
OC   Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cytoplasmic poly(A) RNA polymerase that adds successive
CC       AMP monomers to the 3'-end of specific RNAs, forming a poly(A)
CC       tail. In contrast to the canonical nuclear poly(A) RNA polymerase,
CC       it only adds poly(A) to selected cytoplasmic mRNAs during oocyte
CC       maturation. Plays a central role during oocyte maturation by
CC       mediating polyadenylation of dormant mRNAs, which contain
CC       5'AAUAAA-3' sequence in their 3'-UTR. In immature oocytes,
CC       polyadenylation of poly(A) tails is counteracted by the
CC       ribonuclease parn. During maturation parn is excluded from the
CC       ribonucleoprotein complex, allowing poly(A) elongation and
CC       activation of mRNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + RNA(n) = diphosphate + RNA(n+1).
CC   -!- SUBUNIT: Component of a complex at least composed of cpeb1, cpsf1,
CC       papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte
CC       maturation, parn is expelled from the complex. Interacts with rbm9
CC       (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q0VFA3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q0VFA3-2; Sequence=VSP_034327;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-B-like family. GLD2
CC       subfamily.
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DR   EMBL; BC118909; AAI18910.1; -; mRNA.
DR   EMBL; BC136215; AAI36216.1; -; mRNA.
DR   RefSeq; NP_001072915.1; -.
DR   UniGene; Str.52621; -.
DR   GeneID; 780377; -.
DR   KEGG; xtr:780377; -.
DR   Xenbase; XB-FEAT-1217407; papd4.
DR   HOVERGEN; Q0VFA3; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:EC.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
DR   GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR   InterPro; IPR002934; Nucleotidyltransferase.
DR   InterPro; IPR002058; PAP_assoc.
DR   Pfam; PF01909; NTP_transf_2; 1.
DR   Pfam; PF03828; PAP_assoc; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Cytoplasm; Developmental protein;
KW   Differentiation; mRNA processing; Nucleotide-binding; Oogenesis;
KW   Transferase.
FT   CHAIN         1    528       Poly(A) RNA polymerase GLD2.
FT                                /FTId=PRO_0000341555.
FT   ACT_SITE    261    261       By similarity.
FT   VAR_SEQ      35     39       Missing (in isoform 2).
FT                                /FTId=VSP_034327.
SQ   SEQUENCE   528 AA;  60540 MW;  C7324D94F2546469 CRC64;
     MYPNSPSQGR IPLPLPCEQQ QPPLEQSQEQ PLQPQPLQPQ QASGYLSKLP VSVAPELLSP
     EQFIQASINL HNNVNFARML MNANLLAVPP VSPPPWSYRD QSPLISPASP SSSFQNRKRR
     SDEGNIAYDV KRQKFQSPQE QTVNHQAVPL RGDLGCSYPG SPAFPLLQSP SPPVLKGHVP
     NSGECWLYDH VDTTLPVAKD KLSKQILELF QALQQQVCDL KKKDICRAEL QREIQQIFPQ
     SRLYLVGSSL NGFGTRSSDA DLCLVLKDEP MNQHTEARHI LSLLHKHFYT RLSYIERPQF
     IKAKVPIVKF RDKVSGAEFD LNVNNVVGIR NTFLLRTYAY IENRVRPLVL VIKMWANYHG
     LNDASRGTLS SYTLVLMALH YLQTLPEPII PSLQKKYPEC FDSTMQLHLV HHAPRNIPKY
     LSKNETPLGD LLLGFLKYFA IEFDWSKDII SVREAKALPR SDDYEWRNKF ICVEEPYDRT
     NTARAVYERQ KFDMIRAEFL RAWVALRDNR DLYSLLPWKG IMKKMNSL
//