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Q0VFA3

- GLD2_XENTR

UniProt

Q0VFA3 - GLD2_XENTR

Protein

Poly(A) RNA polymerase GLD2

Gene

papd4

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'-UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation By similarity.By similarity

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi259 – 2591Magnesium or manganese; catalyticBy similarity
    Metal bindingi261 – 2611Magnesium or manganese; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. oogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Transferase

    Keywords - Biological processi

    Differentiation, mRNA processing, Oogenesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
    Alternative name(s):
    PAP-associated domain-containing protein 4
    Gene namesi
    Name:papd4
    Synonyms:gld2
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-1217408. papd4.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 528528Poly(A) RNA polymerase GLD2PRO_0000341555Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiQ0VFA3.

    Interactioni

    Subunit structurei

    Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbm9 and sympk By similarity.By similarity

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000001114.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VFA3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini428 – 48154PAP-associatedAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PAP-associated domain.Curated

    Phylogenomic databases

    eggNOGiCOG5260.
    GeneTreeiENSGT00550000074490.
    HOGENOMiHOG000088606.
    HOVERGENiHBG097533.
    KOiK14079.
    OMAiRAGICEA.
    OrthoDBiEOG7DRJ2S.
    TreeFamiTF315661.

    Family and domain databases

    InterProiIPR002934. Nucleotidyltransferase.
    IPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q0VFA3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MYPNSPSQGR IPLPLPCEQQ QPPLEQSQEQ PLQPQPLQPQ QASGYLSKLP    50
    VSVAPELLSP EQFIQASINL HNNVNFARML MNANLLAVPP VSPPPWSYRD 100
    QSPLISPASP SSSFQNRKRR SDEGNIAYDV KRQKFQSPQE QTVNHQAVPL 150
    RGDLGCSYPG SPAFPLLQSP SPPVLKGHVP NSGECWLYDH VDTTLPVAKD 200
    KLSKQILELF QALQQQVCDL KKKDICRAEL QREIQQIFPQ SRLYLVGSSL 250
    NGFGTRSSDA DLCLVLKDEP MNQHTEARHI LSLLHKHFYT RLSYIERPQF 300
    IKAKVPIVKF RDKVSGAEFD LNVNNVVGIR NTFLLRTYAY IENRVRPLVL 350
    VIKMWANYHG LNDASRGTLS SYTLVLMALH YLQTLPEPII PSLQKKYPEC 400
    FDSTMQLHLV HHAPRNIPKY LSKNETPLGD LLLGFLKYFA IEFDWSKDII 450
    SVREAKALPR SDDYEWRNKF ICVEEPYDRT NTARAVYERQ KFDMIRAEFL 500
    RAWVALRDNR DLYSLLPWKG IMKKMNSL 528
    Length:528
    Mass (Da):60,540
    Last modified:September 5, 2006 - v1
    Checksum:iC7324D94F2546469
    GO
    Isoform 2 (identifier: Q0VFA3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         35-39: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:523
    Mass (Da):59,976
    Checksum:i51A27977D91F6FE9
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei35 – 395Missing in isoform 2. 1 PublicationVSP_034327

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC118909 mRNA. Translation: AAI18910.1.
    BC136215 mRNA. Translation: AAI36216.1.
    RefSeqiNP_001072915.1. NM_001079447.1. [Q0VFA3-1]
    UniGeneiStr.52621.

    Genome annotation databases

    EnsembliENSXETT00000066119; ENSXETP00000062194; ENSXETG00000000518. [Q0VFA3-1]
    GeneIDi780377.
    KEGGixtr:780377.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC118909 mRNA. Translation: AAI18910.1 .
    BC136215 mRNA. Translation: AAI36216.1 .
    RefSeqi NP_001072915.1. NM_001079447.1. [Q0VFA3-1 ]
    UniGenei Str.52621.

    3D structure databases

    ProteinModelPortali Q0VFA3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000001114.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSXETT00000066119 ; ENSXETP00000062194 ; ENSXETG00000000518 . [Q0VFA3-1 ]
    GeneIDi 780377.
    KEGGi xtr:780377.

    Organism-specific databases

    CTDi 167153.
    Xenbasei XB-GENE-1217408. papd4.

    Phylogenomic databases

    eggNOGi COG5260.
    GeneTreei ENSGT00550000074490.
    HOGENOMi HOG000088606.
    HOVERGENi HBG097533.
    KOi K14079.
    OMAi RAGICEA.
    OrthoDBi EOG7DRJ2S.
    TreeFami TF315661.

    Gene expression databases

    Bgeei Q0VFA3.

    Family and domain databases

    InterProi IPR002934. Nucleotidyltransferase.
    IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain.

    Entry informationi

    Entry nameiGLD2_XENTR
    AccessioniPrimary (citable) accession number: Q0VFA3
    Secondary accession number(s): A4IIZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3