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Q0VFA3 (GLD2_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) RNA polymerase GLD2

EC=2.7.7.19
Alternative name(s):
PAP-associated domain-containing protein 4
Gene names
Name:papd4
Synonyms:gld2
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'-UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation By similarity.

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbm9 and sympk By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.

Contains 1 PAP-associated domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q0VFA3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q0VFA3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     35-39: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 528528Poly(A) RNA polymerase GLD2
PRO_0000341555

Regions

Domain428 – 48154PAP-associated

Sites

Metal binding2591Magnesium or manganese; catalytic By similarity
Metal binding2611Magnesium or manganese; catalytic By similarity

Natural variations

Alternative sequence35 – 395Missing in isoform 2.
VSP_034327

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: C7324D94F2546469

FASTA52860,540
        10         20         30         40         50         60 
MYPNSPSQGR IPLPLPCEQQ QPPLEQSQEQ PLQPQPLQPQ QASGYLSKLP VSVAPELLSP 

        70         80         90        100        110        120 
EQFIQASINL HNNVNFARML MNANLLAVPP VSPPPWSYRD QSPLISPASP SSSFQNRKRR 

       130        140        150        160        170        180 
SDEGNIAYDV KRQKFQSPQE QTVNHQAVPL RGDLGCSYPG SPAFPLLQSP SPPVLKGHVP 

       190        200        210        220        230        240 
NSGECWLYDH VDTTLPVAKD KLSKQILELF QALQQQVCDL KKKDICRAEL QREIQQIFPQ 

       250        260        270        280        290        300 
SRLYLVGSSL NGFGTRSSDA DLCLVLKDEP MNQHTEARHI LSLLHKHFYT RLSYIERPQF 

       310        320        330        340        350        360 
IKAKVPIVKF RDKVSGAEFD LNVNNVVGIR NTFLLRTYAY IENRVRPLVL VIKMWANYHG 

       370        380        390        400        410        420 
LNDASRGTLS SYTLVLMALH YLQTLPEPII PSLQKKYPEC FDSTMQLHLV HHAPRNIPKY 

       430        440        450        460        470        480 
LSKNETPLGD LLLGFLKYFA IEFDWSKDII SVREAKALPR SDDYEWRNKF ICVEEPYDRT 

       490        500        510        520 
NTARAVYERQ KFDMIRAEFL RAWVALRDNR DLYSLLPWKG IMKKMNSL 

« Hide

Isoform 2 [UniParc].

Checksum: 51A27977D91F6FE9
Show »

FASTA52359,976

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC118909 mRNA. Translation: AAI18910.1.
BC136215 mRNA. Translation: AAI36216.1.
RefSeqNP_001072915.1. NM_001079447.1.
UniGeneStr.52621.

3D structure databases

ProteinModelPortalQ0VFA3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000001114.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSXETT00000066119; ENSXETP00000062194; ENSXETG00000000518. [Q0VFA3-1]
GeneID780377.
KEGGxtr:780377.

Organism-specific databases

CTD167153.
XenbaseXB-GENE-1217408. papd4.

Phylogenomic databases

eggNOGCOG5260.
GeneTreeENSGT00550000074490.
HOGENOMHOG000088606.
HOVERGENHBG097533.
KOK14079.
OrthoDBEOG7DRJ2S.
TreeFamTF315661.

Gene expression databases

BgeeQ0VFA3.

Family and domain databases

InterProIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLD2_XENTR
AccessionPrimary (citable) accession number: Q0VFA3
Secondary accession number(s): A4IIZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 5, 2006
Last modified: March 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families