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Q0VFA3

- GLD2_XENTR

UniProt

Q0VFA3 - GLD2_XENTR

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Protein
Poly(A) RNA polymerase GLD2
Gene
papd4, gld2
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'-UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation By similarity.

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Magnesium or manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi259 – 2591Magnesium or manganese; catalytic By similarity
Metal bindingi261 – 2611Magnesium or manganese; catalytic By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. oogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Differentiation, mRNA processing, Oogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
Alternative name(s):
PAP-associated domain-containing protein 4
Gene namesi
Name:papd4
Synonyms:gld2
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-1217408. papd4.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Poly(A) RNA polymerase GLD2
PRO_0000341555Add
BLAST

Expressioni

Gene expression databases

BgeeiQ0VFA3.

Interactioni

Subunit structurei

Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbm9 and sympk By similarity.

Protein-protein interaction databases

STRINGi8364.ENSXETP00000001114.

Structurei

3D structure databases

ProteinModelPortaliQ0VFA3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini428 – 48154PAP-associated
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000088606.
HOVERGENiHBG097533.
KOiK14079.
OMAiRAGICEA.
OrthoDBiEOG7DRJ2S.
TreeFamiTF315661.

Family and domain databases

InterProiIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q0VFA3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MYPNSPSQGR IPLPLPCEQQ QPPLEQSQEQ PLQPQPLQPQ QASGYLSKLP    50
VSVAPELLSP EQFIQASINL HNNVNFARML MNANLLAVPP VSPPPWSYRD 100
QSPLISPASP SSSFQNRKRR SDEGNIAYDV KRQKFQSPQE QTVNHQAVPL 150
RGDLGCSYPG SPAFPLLQSP SPPVLKGHVP NSGECWLYDH VDTTLPVAKD 200
KLSKQILELF QALQQQVCDL KKKDICRAEL QREIQQIFPQ SRLYLVGSSL 250
NGFGTRSSDA DLCLVLKDEP MNQHTEARHI LSLLHKHFYT RLSYIERPQF 300
IKAKVPIVKF RDKVSGAEFD LNVNNVVGIR NTFLLRTYAY IENRVRPLVL 350
VIKMWANYHG LNDASRGTLS SYTLVLMALH YLQTLPEPII PSLQKKYPEC 400
FDSTMQLHLV HHAPRNIPKY LSKNETPLGD LLLGFLKYFA IEFDWSKDII 450
SVREAKALPR SDDYEWRNKF ICVEEPYDRT NTARAVYERQ KFDMIRAEFL 500
RAWVALRDNR DLYSLLPWKG IMKKMNSL 528
Length:528
Mass (Da):60,540
Last modified:September 5, 2006 - v1
Checksum:iC7324D94F2546469
GO
Isoform 2 (identifier: Q0VFA3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     35-39: Missing.

Note: No experimental confirmation available.

Show »
Length:523
Mass (Da):59,976
Checksum:i51A27977D91F6FE9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei35 – 395Missing in isoform 2.
VSP_034327

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC118909 mRNA. Translation: AAI18910.1.
BC136215 mRNA. Translation: AAI36216.1.
RefSeqiNP_001072915.1. NM_001079447.1. [Q0VFA3-1]
UniGeneiStr.52621.

Genome annotation databases

EnsembliENSXETT00000066119; ENSXETP00000062194; ENSXETG00000000518. [Q0VFA3-1]
GeneIDi780377.
KEGGixtr:780377.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC118909 mRNA. Translation: AAI18910.1 .
BC136215 mRNA. Translation: AAI36216.1 .
RefSeqi NP_001072915.1. NM_001079447.1. [Q0VFA3-1 ]
UniGenei Str.52621.

3D structure databases

ProteinModelPortali Q0VFA3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000001114.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSXETT00000066119 ; ENSXETP00000062194 ; ENSXETG00000000518 . [Q0VFA3-1 ]
GeneIDi 780377.
KEGGi xtr:780377.

Organism-specific databases

CTDi 167153.
Xenbasei XB-GENE-1217408. papd4.

Phylogenomic databases

eggNOGi COG5260.
GeneTreei ENSGT00550000074490.
HOGENOMi HOG000088606.
HOVERGENi HBG097533.
KOi K14079.
OMAi RAGICEA.
OrthoDBi EOG7DRJ2S.
TreeFami TF315661.

Gene expression databases

Bgeei Q0VFA3.

Family and domain databases

InterProi IPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.

Entry informationi

Entry nameiGLD2_XENTR
AccessioniPrimary (citable) accession number: Q0VFA3
Secondary accession number(s): A4IIZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: September 5, 2006
Last modified: July 9, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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