Q0VFA3 (GLD2_XENTR) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 42.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Poly(A) RNA polymerase GLD2 EC=2.7.7.19 Alternative name(s): PAP-associated domain-containing protein 4 | ||||
| Gene names |
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| Organism | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome] | ||||
| Taxonomic identifier | 8364 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Protein attributes
| Sequence length | 528 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'-UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation By similarity. |
| Catalytic activity | ATP + RNA(n) = diphosphate + RNA(n+1). |
| Cofactor | Magnesium or manganese By similarity. |
| Subunit structure | Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbm9 and sympk By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the DNA polymerase type-B-like family. GLD2 subfamily. Contains 1 PAP-associated domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q0VFA3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q0VFA3-2) The sequence of this isoform differs from the canonical sequence as follows: 35-39: Missing. | ||||||
| Note: No experimental confirmation available. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 528 | 528 | Poly(A) RNA polymerase GLD2 | PRO_0000341555 | |||||
Regions | |||||||||
| Domain | 428 – 481 | 54 | PAP-associated | ||||||
Sites | |||||||||
| Metal binding | 259 | 1 | Magnesium or manganese; catalytic By similarity | ||||||
| Metal binding | 261 | 1 | Magnesium or manganese; catalytic By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 35 – 39 | 5 | Missing in isoform 2. | VSP_034327 | |||||
Sequences
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References
| [1] | NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC118909 mRNA. Translation: AAI18910.1. BC136215 mRNA. Translation: AAI36216.1. |
| RefSeq | NP_001072915.1. NM_001079447.1. |
| UniGene | Str.52621. |
3D structure databases | |
| ProteinModelPortal | Q0VFA3. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 8364.ENSXETP00000001114. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSXETT00000066119; ENSXETP00000062194; ENSXETG00000000518. |
| GeneID | 780377. |
| KEGG | xtr:780377. |
Organism-specific databases | |
| CTD | 167153. |
| Xenbase | XB-GENE-1217408. papd4. |
Phylogenomic databases | |
| eggNOG | COG5260. |
| GeneTree | ENSGT00550000074490. |
| HOGENOM | HOG000088606. |
| HOVERGEN | HBG097533. |
| KO | K14079. |
Gene expression databases | |
| Bgee | Q0VFA3. |
Family and domain databases | |
| InterPro | IPR002934. Nucleotidyltransferase. IPR002058. PAP_assoc. [Graphical view] |
| Pfam | PF01909. NTP_transf_2. 1 hit. PF03828. PAP_assoc. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GLD2_XENTR | ||||||||
| Accession | Primary (citable) accession number: Q0VFA3 Secondary accession number(s): A4IIZ6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |