Reviewed, UniProtKB/Swiss-Prot Q0VFA3 (GLD2_XENTR)

Names and origin

Protein names

Recommended name:
    Poly(A) RNA polymerase GLD2
    EC=2.7.7.19
Alternative name(s):
    PAP-associated domain-containing protein 4

Gene names

Name:	papd4
Synonyms:	gld2

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'-UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs By similarity.
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbm9 By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.

Ontologies

Keywords
Biological process	Differentiation Oogenesis mRNA processing
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Transferase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q0VFA3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q0VFA3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     35-39: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Poly(A) RNA polymerase GLD2		PRO_0000341555
Sites
	Active site	261	1	By similarity
Natural variations
	Alternative sequence	35 – 39	5	Missing in isoform 2.		VSP_034327

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: C7324D94F2546469	FASTA	528	60,540
	10 20 30 40 50 60 MYPNSPSQGR IPLPLPCEQQ QPPLEQSQEQ PLQPQPLQPQ QASGYLSKLP VSVAPELLSP 70 80 90 100 110 120 EQFIQASINL HNNVNFARML MNANLLAVPP VSPPPWSYRD QSPLISPASP SSSFQNRKRR 130 140 150 160 170 180 SDEGNIAYDV KRQKFQSPQE QTVNHQAVPL RGDLGCSYPG SPAFPLLQSP SPPVLKGHVP 190 200 210 220 230 240 NSGECWLYDH VDTTLPVAKD KLSKQILELF QALQQQVCDL KKKDICRAEL QREIQQIFPQ 250 260 270 280 290 300 SRLYLVGSSL NGFGTRSSDA DLCLVLKDEP MNQHTEARHI LSLLHKHFYT RLSYIERPQF 310 320 330 340 350 360 IKAKVPIVKF RDKVSGAEFD LNVNNVVGIR NTFLLRTYAY IENRVRPLVL VIKMWANYHG 370 380 390 400 410 420 LNDASRGTLS SYTLVLMALH YLQTLPEPII PSLQKKYPEC FDSTMQLHLV HHAPRNIPKY 430 440 450 460 470 480 LSKNETPLGD LLLGFLKYFA IEFDWSKDII SVREAKALPR SDDYEWRNKF ICVEEPYDRT 490 500 510 520 NTARAVYERQ KFDMIRAEFL RAWVALRDNR DLYSLLPWKG IMKKMNSL
	Isoform 2. Checksum: 51A27977D91F6FE9	FASTA	523	59,976
	10 20 30 40 50 60 MYPNSPSQGR IPLPLPCEQQ QPPLEQSQEQ PLQPQQASGY LSKLPVSVAP ELLSPEQFIQ 70 80 90 100 110 120 ASINLHNNVN FARMLMNANL LAVPPVSPPP WSYRDQSPLI SPASPSSSFQ NRKRRSDEGN 130 140 150 160 170 180 IAYDVKRQKF QSPQEQTVNH QAVPLRGDLG CSYPGSPAFP LLQSPSPPVL KGHVPNSGEC 190 200 210 220 230 240 WLYDHVDTTL PVAKDKLSKQ ILELFQALQQ QVCDLKKKDI CRAELQREIQ QIFPQSRLYL 250 260 270 280 290 300 VGSSLNGFGT RSSDADLCLV LKDEPMNQHT EARHILSLLH KHFYTRLSYI ERPQFIKAKV 310 320 330 340 350 360 PIVKFRDKVS GAEFDLNVNN VVGIRNTFLL RTYAYIENRV RPLVLVIKMW ANYHGLNDAS 370 380 390 400 410 420 RGTLSSYTLV LMALHYLQTL PEPIIPSLQK KYPECFDSTM QLHLVHHAPR NIPKYLSKNE 430 440 450 460 470 480 TPLGDLLLGF LKYFAIEFDW SKDIISVREA KALPRSDDYE WRNKFICVEE PYDRTNTARA 490 500 510 520 VYERQKFDMI RAEFLRAWVA LRDNRDLYSL LPWKGIMKKM NSL

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC118909 mRNA. Translation: AAI18910.1. BC136215 mRNA. Translation: AAI36216.1.
RefSeq	NP_001072915.1.
UniGene	Str.52621
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	780377.
KEGG	xtr:780377.
Organism-specific databases
Xenbase	XB-FEAT-1217407. papd4.
Phylogenomic databases
HOVERGEN	Q0VFA3.
Family and domain databases
InterPro	IPR002934. Nucleotidyltransferase. IPR002058. PAP_assoc. [Graphical view]
Pfam	PF01909. NTP_transf_2. 1 hit. PF03828. PAP_assoc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLD2_XENTR

Accession

Primary (citable) accession number: Q0VFA3
Secondary accession number(s): A4IIZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project