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Protein

Protein INCA1

Gene

INCA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. cyclin binding Source: MGI
  2. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: MGI

GO - Biological processi

  1. negative regulation of cell proliferation Source: Ensembl
  2. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  3. positive regulation of apoptotic signaling pathway Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein INCA1
Alternative name(s):
Inhibitor of CDK interacting with cyclin A1
Gene namesi
Name:INCA1
ORF Names:HSD45
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:32224. INCA1.

Subcellular locationi

  1. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231S → A: Loss of phosphorylation site. 1 Publication
Mutagenesisi182 – 1821T → A: Reduced phosphorylation. Phosphorylation is almost abolished; when associated with A-191. 1 Publication
Mutagenesisi191 – 1911S → A: Strongly reduced phosphorylation. Phosphorylation is almost abolished; when associated with A-182. 1 Publication
Mutagenesisi194 – 1941S → A: Reduced phosphorylation. 1 Publication

Organism-specific databases

PharmGKBiPA165431930.

Polymorphism and mutation databases

BioMutaiINCA1.
DMDMi121940471.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236Protein INCA1PRO_0000331513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Phosphoserine1 Publication
Modified residuei182 – 1821Phosphothreonine1 Publication
Modified residuei191 – 1911Phosphoserine1 Publication
Modified residuei194 – 1941Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated when part of a complex with CCNA1 and CDK2. Strongly phosphorylated by CDK2 on its C-terminal region spanning amino acid 149-221. Less intensively phosphorylated by CDK2 on its first 75 amino acid residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ0VD86.
PRIDEiQ0VD86.

PTM databases

PhosphoSiteiQ0VD86.

Expressioni

Tissue specificityi

Detected in testis, and at lower levels in ovary. Detected at very low levels in testis tumors.1 Publication

Gene expression databases

BgeeiQ0VD86.
GenevestigatoriQ0VD86.

Organism-specific databases

HPAiHPA044359.

Interactioni

Subunit structurei

Interacts with CCNA1. Identified in a complex with CCNA1 and CDK2. Interacts with ZNF16; the interaction inhibits INCA1 activity and induces the cell cycle process.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARMC7Q9H6L43EBI-6509505,EBI-742909
CABP5Q9NP863EBI-6509505,EBI-10311131
CDC23Q9UJX23EBI-6509505,EBI-396137
CDKN2DP552733EBI-6509505,EBI-745859
CHCHD2Q9Y6H13EBI-6509505,EBI-2321769
COL8A1P276583EBI-6509505,EBI-747133
CTNNBIP1Q9NSA33EBI-6509505,EBI-747082
DMRT3Q9NQL93EBI-6509505,EBI-9679045
EPHB6O15197-23EBI-6509505,EBI-10182490
FAM103A1Q9BTL33EBI-6509505,EBI-744023
FHL2Q141923EBI-6509505,EBI-701903
GRB2P629933EBI-6509505,EBI-401755
HAUS1Q96CS23EBI-6509505,EBI-2514791
NEK6Q9HC983EBI-6509505,EBI-740364
OSGIN1Q9UJX03EBI-6509505,EBI-9057006
PATE1Q8WXA23EBI-6509505,EBI-10277790
PTGER3Q147X83EBI-6509505,EBI-10234038
RBPMSQ930623EBI-6509505,EBI-740322
SAP18O004223EBI-6509505,EBI-1044156
SCNM1Q9BWG63EBI-6509505,EBI-748391
SEC14L4Q9UDX33EBI-6509505,EBI-10320311
SPG21Q9NZD83EBI-6509505,EBI-742688
STAMBPL1Q96FJ03EBI-6509505,EBI-745021
STK16O757163EBI-6509505,EBI-749295
TRIP6Q156543EBI-6509505,EBI-742327
UBE2D4Q9Y2X83EBI-6509505,EBI-745527
UBXN2BQ14CS03EBI-6509505,EBI-1993619
VPS28Q548N13EBI-6509505,EBI-10243107
ZMYND19Q96E353EBI-6509505,EBI-746595
ZNF16P170202EBI-6509505,EBI-3921553

Protein-protein interaction databases

BioGridi132642. 33 interactions.
IntActiQ0VD86. 30 interactions.
STRINGi9606.ENSP00000347129.

Structurei

3D structure databases

ProteinModelPortaliQ0VD86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi90 – 956Nuclear localization signalSequence Analysis

Sequence similaritiesi

Belongs to the INCA family.Curated

Phylogenomic databases

eggNOGiNOG84185.
GeneTreeiENSGT00390000000242.
HOGENOMiHOG000113068.
HOVERGENiHBG095410.
InParanoidiQ0VD86.
OMAiQLLWSPW.
OrthoDBiEOG7ZWD3F.
PhylomeDBiQ0VD86.
TreeFamiTF343431.

Family and domain databases

InterProiIPR026238. INCA1.
[Graphical view]
PfamiPF15142. INCA1. 1 hit.
[Graphical view]
PRINTSiPR02102. PROTEININCA1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q0VD86-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQVQDDGVNL IPFAKCSRVV SRSPPPRLPS QSLRPMPQRY GDVFWKNLNQ
60 70 80 90 100
RPTPTWLEEQ HIPPMLRATG CSQLGLYPPE QLPPPEMLWR RKKRRPCLEG
110 120 130 140 150
MQQQGLGGVP ARVRAVTYHL EDLRRRQSII NELKKAQWGS SGAASEPVVL
160 170 180 190 200
GEEGCGFPST NEYPDLEEER ATYPQEEDRF LTPGRAQLLW SPWSPLDQEE
210 220 230
ACASRQLHSL ASFSTVTARR NPLHNPWGME LAASEE
Length:236
Mass (Da):26,817
Last modified:September 5, 2006 - v1
Checksum:iA9808D24144AB07D
GO
Isoform 2 (identifier: Q0VD86-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-81: Missing.

Show »
Length:221
Mass (Da):25,216
Checksum:iA1070CBDD2CA4650
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531E → D in AAT09152 (PubMed:15159402).Curated
Sequence conflicti153 – 1531E → D in AAT09153 (PubMed:15159402).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei67 – 8115Missing in isoform 2. 2 PublicationsVSP_033235Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY601906 mRNA. Translation: AAT09152.1.
AY601907 mRNA. Translation: AAT09153.1.
AY604176 mRNA. Translation: AAT36739.1.
BC119781 mRNA. Translation: AAI19782.1.
BC122873 mRNA. Translation: AAI22874.1.
CCDSiCCDS11064.1. [Q0VD86-2]
CCDS54074.1. [Q0VD86-1]
RefSeqiNP_001161457.1. NM_001167985.1. [Q0VD86-2]
NP_001161458.1. NM_001167986.1. [Q0VD86-1]
NP_001161459.1. NM_001167987.1. [Q0VD86-1]
NP_998891.2. NM_213726.2. [Q0VD86-2]
XP_005256684.1. XM_005256627.2. [Q0VD86-1]
XP_005256685.1. XM_005256628.3. [Q0VD86-1]
XP_005256686.1. XM_005256629.1. [Q0VD86-2]
XP_006721580.1. XM_006721517.1. [Q0VD86-1]
XP_006721581.1. XM_006721518.1. [Q0VD86-2]
XP_006721582.1. XM_006721519.2. [Q0VD86-2]
UniGeneiHs.658926.

Genome annotation databases

EnsembliENST00000355025; ENSP00000347129; ENSG00000196388. [Q0VD86-2]
ENST00000574617; ENSP00000458316; ENSG00000196388. [Q0VD86-1]
ENST00000575780; ENSP00000461844; ENSG00000196388. [Q0VD86-2]
ENST00000576820; ENSP00000460673; ENSG00000196388. [Q0VD86-1]
GeneIDi388324.
KEGGihsa:388324.
UCSCiuc002gaj.3. human. [Q0VD86-1]
uc002gal.3. human. [Q0VD86-2]

Polymorphism and mutation databases

BioMutaiINCA1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY601906 mRNA. Translation: AAT09152.1.
AY601907 mRNA. Translation: AAT09153.1.
AY604176 mRNA. Translation: AAT36739.1.
BC119781 mRNA. Translation: AAI19782.1.
BC122873 mRNA. Translation: AAI22874.1.
CCDSiCCDS11064.1. [Q0VD86-2]
CCDS54074.1. [Q0VD86-1]
RefSeqiNP_001161457.1. NM_001167985.1. [Q0VD86-2]
NP_001161458.1. NM_001167986.1. [Q0VD86-1]
NP_001161459.1. NM_001167987.1. [Q0VD86-1]
NP_998891.2. NM_213726.2. [Q0VD86-2]
XP_005256684.1. XM_005256627.2. [Q0VD86-1]
XP_005256685.1. XM_005256628.3. [Q0VD86-1]
XP_005256686.1. XM_005256629.1. [Q0VD86-2]
XP_006721580.1. XM_006721517.1. [Q0VD86-1]
XP_006721581.1. XM_006721518.1. [Q0VD86-2]
XP_006721582.1. XM_006721519.2. [Q0VD86-2]
UniGeneiHs.658926.

3D structure databases

ProteinModelPortaliQ0VD86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi132642. 33 interactions.
IntActiQ0VD86. 30 interactions.
STRINGi9606.ENSP00000347129.

PTM databases

PhosphoSiteiQ0VD86.

Polymorphism and mutation databases

BioMutaiINCA1.
DMDMi121940471.

Proteomic databases

PaxDbiQ0VD86.
PRIDEiQ0VD86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355025; ENSP00000347129; ENSG00000196388. [Q0VD86-2]
ENST00000574617; ENSP00000458316; ENSG00000196388. [Q0VD86-1]
ENST00000575780; ENSP00000461844; ENSG00000196388. [Q0VD86-2]
ENST00000576820; ENSP00000460673; ENSG00000196388. [Q0VD86-1]
GeneIDi388324.
KEGGihsa:388324.
UCSCiuc002gaj.3. human. [Q0VD86-1]
uc002gal.3. human. [Q0VD86-2]

Organism-specific databases

CTDi388324.
GeneCardsiGC17M004891.
H-InvDBHIX0027201.
HGNCiHGNC:32224. INCA1.
HPAiHPA044359.
neXtProtiNX_Q0VD86.
PharmGKBiPA165431930.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG84185.
GeneTreeiENSGT00390000000242.
HOGENOMiHOG000113068.
HOVERGENiHBG095410.
InParanoidiQ0VD86.
OMAiQLLWSPW.
OrthoDBiEOG7ZWD3F.
PhylomeDBiQ0VD86.
TreeFamiTF343431.

Miscellaneous databases

GenomeRNAii388324.
NextBioi101955.
PROiQ0VD86.

Gene expression databases

BgeeiQ0VD86.
GenevestigatoriQ0VD86.

Family and domain databases

InterProiIPR026238. INCA1.
[Graphical view]
PfamiPF15142. INCA1. 1 hit.
[Graphical view]
PRINTSiPR02102. PROTEININCA1.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CCNA1, IDENTIFICATION IN A COMPLEX WITH CCNA1 AND CDK2, PHOSPHORYLATION AT SER-23; THR-182; SER-191 AND SER-194, MUTAGENESIS OF SER-23; THR-182; SER-191 AND SER-194, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "A new spermatogenesis-related gene."
    Hu T.H., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Zinc finger protein HZF1 promotes K562 cell proliferation by interacting with and inhibiting INCA1."
    Li X.B., Chen J., Deng M.J., Wang F., Du Z.W., Zhang J.W.
    Mol. Med. Report. 4:1131-1137(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZNF16.

Entry informationi

Entry nameiINCA1_HUMAN
AccessioniPrimary (citable) accession number: Q0VD86
Secondary accession number(s): Q6J273, Q6PKN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: April 29, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.