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Q0VD86 (INCA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein INCA1
Alternative name(s):
Inhibitor of CDK interacting with cyclin A1
Gene names
Name:INCA1
ORF Names:HSD45
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with CCNA1. Identified in a complex with CCNA1 and CDK2. Interacts with ZNF16; the interaction inhibits INCA1 activity and induces the cell cycle process. Ref.1 Ref.4

Subcellular location

Nucleus Ref.1.

Tissue specificity

Detected in testis, and at lower levels in ovary. Detected at very low levels in testis tumors. Ref.1

Post-translational modification

Phosphorylated when part of a complex with CCNA1 and CDK2. Strongly phosphorylated by CDK2 on its C-terminal region spanning amino acid 149-221. Less intensively phosphorylated by CDK2 on its first 75 amino acid residues. Ref.1

Sequence similarities

Belongs to the INCA family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ZNF16P170202EBI-6509505,EBI-3921553

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q0VD86-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q0VD86-2)

The sequence of this isoform differs from the canonical sequence as follows:
     67-81: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 236236Protein INCA1
PRO_0000331513

Regions

Motif90 – 956Nuclear localization signal Potential

Amino acid modifications

Modified residue231Phosphoserine Ref.1
Modified residue1821Phosphothreonine Ref.1
Modified residue1911Phosphoserine Ref.1
Modified residue1941Phosphoserine Probable

Natural variations

Alternative sequence67 – 8115Missing in isoform 2.
VSP_033235

Experimental info

Mutagenesis231S → A: Loss of phosphorylation site. Ref.1
Mutagenesis1821T → A: Reduced phosphorylation. Phosphorylation is almost abolished; when associated with A-191. Ref.1
Mutagenesis1911S → A: Strongly reduced phosphorylation. Phosphorylation is almost abolished; when associated with A-182. Ref.1
Mutagenesis1941S → A: Reduced phosphorylation. Ref.1
Sequence conflict1531E → D in AAT09152. Ref.1
Sequence conflict1531E → D in AAT09153. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: A9808D24144AB07D

FASTA23626,817
        10         20         30         40         50         60 
MQVQDDGVNL IPFAKCSRVV SRSPPPRLPS QSLRPMPQRY GDVFWKNLNQ RPTPTWLEEQ 

        70         80         90        100        110        120 
HIPPMLRATG CSQLGLYPPE QLPPPEMLWR RKKRRPCLEG MQQQGLGGVP ARVRAVTYHL 

       130        140        150        160        170        180 
EDLRRRQSII NELKKAQWGS SGAASEPVVL GEEGCGFPST NEYPDLEEER ATYPQEEDRF 

       190        200        210        220        230 
LTPGRAQLLW SPWSPLDQEE ACASRQLHSL ASFSTVTARR NPLHNPWGME LAASEE 

« Hide

Isoform 2 [UniParc].

Checksum: A1070CBDD2CA4650
Show »

FASTA22125,216

References

« Hide 'large scale' references
[1]"Identification of interaction partners and substrates of the cyclin A1-CDK2 complex."
Diederichs S., Baeumer N., Ji P., Metzelder S.K., Idos G.E., Cauvet T., Wang W., Moeller M., Pierschalski S., Gromoll J., Schrader M.G., Koeffler H.P., Berdel W.E., Serve H., Mueller-Tidow C.
J. Biol. Chem. 279:33727-33741(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CCNA1, IDENTIFICATION IN A COMPLEX WITH CCNA1 AND CDK2, PHOSPHORYLATION AT SER-23; THR-182; SER-191 AND SER-194, MUTAGENESIS OF SER-23; THR-182; SER-191 AND SER-194, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Testis.
[2]"A new spermatogenesis-related gene."
Hu T.H., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.
Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]"Zinc finger protein HZF1 promotes K562 cell proliferation by interacting with and inhibiting INCA1."
Li X.B., Chen J., Deng M.J., Wang F., Du Z.W., Zhang J.W.
Mol. Med. Report. 4:1131-1137(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZNF16.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY601906 mRNA. Translation: AAT09152.1.
AY601907 mRNA. Translation: AAT09153.1.
AY604176 mRNA. Translation: AAT36739.1.
BC119781 mRNA. Translation: AAI19782.1.
BC122873 mRNA. Translation: AAI22874.1.
RefSeqNP_001161457.1. NM_001167985.1.
NP_001161458.1. NM_001167986.1.
NP_001161459.1. NM_001167987.1.
NP_998891.2. NM_213726.2.
XP_005256684.1. XM_005256627.1.
XP_005256685.1. XM_005256628.2.
XP_005256686.1. XM_005256629.1.
UniGeneHs.658926.

3D structure databases

ProteinModelPortalQ0VD86.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid132642. 2 interactions.
IntActQ0VD86. 1 interaction.
STRING9606.ENSP00000347129.

PTM databases

PhosphoSiteQ0VD86.

Polymorphism databases

DMDM121940471.

Proteomic databases

PaxDbQ0VD86.
PRIDEQ0VD86.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000355025; ENSP00000347129; ENSG00000196388. [Q0VD86-2]
ENST00000396829; ENSP00000380041; ENSG00000196388. [Q0VD86-1]
ENST00000574617; ENSP00000458316; ENSG00000196388. [Q0VD86-1]
ENST00000575780; ENSP00000461844; ENSG00000196388. [Q0VD86-2]
ENST00000576820; ENSP00000460673; ENSG00000196388. [Q0VD86-1]
GeneID388324.
KEGGhsa:388324.
UCSCuc002gaj.3. human. [Q0VD86-1]
uc002gal.3. human. [Q0VD86-2]

Organism-specific databases

CTD388324.
GeneCardsGC17M004891.
H-InvDBHIX0027201.
HGNCHGNC:32224. INCA1.
HPAHPA044359.
neXtProtNX_Q0VD86.
PharmGKBPA165431930.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG84185.
HOGENOMHOG000113068.
HOVERGENHBG095410.
InParanoidQ0VD86.
OMAQLLWSPW.
OrthoDBEOG7ZWD3F.
PhylomeDBQ0VD86.
TreeFamTF343431.

Gene expression databases

BgeeQ0VD86.
GenevestigatorQ0VD86.

Family and domain databases

InterProIPR026238. INCA1.
[Graphical view]
PfamPF15142. INCA1. 1 hit.
[Graphical view]
PRINTSPR02102. PROTEININCA1.
ProtoNetSearch...

Other

GenomeRNAi388324.
NextBio101955.
PROQ0VD86.

Entry information

Entry nameINCA1_HUMAN
AccessionPrimary (citable) accession number: Q0VD86
Secondary accession number(s): Q6J273, Q6PKN9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM