Q0VD86 (INCA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein INCA1

Alternative name(s):
Inhibitor of CDK interacting with cyclin A1

Gene names

Name:	INCA1
ORF Names:	HSD45

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with CCNA1. Identified in a complex with CCNA1 and CDK2. Ref.1
Subcellular location	Nucleus Ref.1.
Tissue specificity	Detected in testis, and at lower levels in ovary. Detected at very low levels in testis tumors. Ref.1
Post-translational modification	Phosphorylated when part of a complex with CCNA1 and CDK2. Strongly phosphorylated by CDK2 on its C-terminal region spanning amino acid 149-221. Less intensively phosphorylated by CDK2 on its first 75 amino acid residues. Ref.1
Sequence similarities	Belongs to the INCA family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay PubMed 21540187. Source: MGI
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular_function	cyclin binding Inferred from direct assay PubMed 21540187. Source: MGI cyclin-dependent protein serine/threonine kinase inhibitor activity Inferred from direct assay PubMed 21540187. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ZNF16	P17020	2	EBI-6509505,EBI-3921553

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q0VD86-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q0VD86-2)

The sequence of this isoform differs from the canonical sequence as follows:
     67-81: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Protein INCA1		PRO_0000331513
Regions
	Motif	90 – 95	6	Nuclear localization signal Potential
Amino acid modifications
	Modified residue	23	1	Phosphoserine Ref.1
	Modified residue	182	1	Phosphothreonine Ref.1
	Modified residue	191	1	Phosphoserine Ref.1
	Modified residue	194	1	Phosphoserine Probable
Natural variations
	Alternative sequence	67 – 81	15	Missing in isoform 2.		VSP_033235
Experimental info
	Mutagenesis	23	1	S → A: Loss of phosphorylation site. Ref.1
	Mutagenesis	182	1	T → A: Reduced phosphorylation. Phosphorylation is almost abolished; when associated with A-191. Ref.1
	Mutagenesis	191	1	S → A: Strongly reduced phosphorylation. Phosphorylation is almost abolished; when associated with A-182. Ref.1
	Mutagenesis	194	1	S → A: Reduced phosphorylation. Ref.1
	Sequence conflict	153	1	E → D in AAT09152. Ref.1
	Sequence conflict	153	1	E → D in AAT09153. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: A9808D24144AB07D	FASTA	236	26,817
	10 20 30 40 50 60 MQVQDDGVNL IPFAKCSRVV SRSPPPRLPS QSLRPMPQRY GDVFWKNLNQ RPTPTWLEEQ 70 80 90 100 110 120 HIPPMLRATG CSQLGLYPPE QLPPPEMLWR RKKRRPCLEG MQQQGLGGVP ARVRAVTYHL 130 140 150 160 170 180 EDLRRRQSII NELKKAQWGS SGAASEPVVL GEEGCGFPST NEYPDLEEER ATYPQEEDRF 190 200 210 220 230 LTPGRAQLLW SPWSPLDQEE ACASRQLHSL ASFSTVTARR NPLHNPWGME LAASEE
	Isoform 2 [UniParc]. Checksum: A1070CBDD2CA4650	FASTA	221	25,216
	10 20 30 40 50 60 MQVQDDGVNL IPFAKCSRVV SRSPPPRLPS QSLRPMPQRY GDVFWKNLNQ RPTPTWLEEQ 70 80 90 100 110 120 HIPPMLLPPP EMLWRRKKRR PCLEGMQQQG LGGVPARVRA VTYHLEDLRR RQSIINELKK 130 140 150 160 170 180 AQWGSSGAAS EPVVLGEEGC GFPSTNEYPD LEEERATYPQ EEDRFLTPGR AQLLWSPWSP 190 200 210 220 LDQEEACASR QLHSLASFST VTARRNPLHN PWGMELAASE E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of interaction partners and substrates of the cyclin A1-CDK2 complex." Diederichs S., Baeumer N., Ji P., Metzelder S.K., Idos G.E., Cauvet T., Wang W., Moeller M., Pierschalski S., Gromoll J., Schrader M.G., Koeffler H.P., Berdel W.E., Serve H., Mueller-Tidow C. J. Biol. Chem. 279:33727-33741(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CCNA1, IDENTIFICATION IN A COMPLEX WITH CCNA1 AND CDK2, PHOSPHORYLATION AT SER-23; THR-182; SER-191 AND SER-194, MUTAGENESIS OF SER-23; THR-182; SER-191 AND SER-194, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"A new spermatogenesis-related gene." Hu T.H., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY601906 mRNA. Translation: AAT09152.1. AY601907 mRNA. Translation: AAT09153.1. AY604176 mRNA. Translation: AAT36739.1. BC119781 mRNA. Translation: AAI19782.1. BC122873 mRNA. Translation: AAI22874.1.
IPI	IPI00412780. IPI00874179.
RefSeq	NP_001161457.1. NM_001167985.1. NP_001161458.1. NM_001167986.1. NP_001161459.1. NM_001167987.1. NP_998891.2. NM_213726.2.
UniGene	Hs.658926.
3D structure databases
ProteinModelPortal	Q0VD86.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q0VD86. 1 interaction.
STRING	9606.ENSP00000347129.
PTM databases
PhosphoSite	Q0VD86.
Polymorphism databases
DMDM	121940471.
Proteomic databases
PaxDb	Q0VD86.
PRIDE	Q0VD86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000355025; ENSP00000347129; ENSG00000196388. ENST00000396829; ENSP00000380041; ENSG00000196388. ENST00000574617; ENSP00000458316; ENSG00000196388. ENST00000575780; ENSP00000461844; ENSG00000196388. ENST00000576820; ENSP00000460673; ENSG00000196388.
GeneID	388324.
KEGG	hsa:388324.
UCSC	uc002gaj.3. human. uc002gal.3. human.
Organism-specific databases
CTD	388324.
GeneCards	GC17M004891.
H-InvDB	HIX0027201.
HGNC	HGNC:32224. INCA1.
HPA	HPA044359.
neXtProt	NX_Q0VD86.
PharmGKB	PA165431930.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG84185.
HOGENOM	HOG000113068.
HOVERGEN	HBG095410.
InParanoid	Q0VD86.
OMA	LLWSPWS.
Gene expression databases
Bgee	Q0VD86.
Genevestigator	Q0VD86.
Family and domain databases
InterPro	IPR026238. INCA1. [Graphical view]
PRINTS	PR02102. PROTEININCA1.
ProtoNet	Search...
Other
GenomeRNAi	388324.
NextBio	101955.

Entry information

Entry name

INCA1_HUMAN

Accession

Primary (citable) accession number: Q0VD86
Secondary accession number(s): Q6J273, Q6PKN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.