Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q0VD48

- VPS4B_BOVIN

UniProt

Q0VD48 - VPS4B_BOVIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Vacuolar protein sorting-associated protein 4B

Gene

VPS4B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (lentiviruses). Involved in cytokinesis (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi174 – 1818ATPSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: Ensembl
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. endosome organization Source: Ensembl
  4. endosome to lysosome transport via multivesicular body sorting pathway Source: Ensembl
  5. intracellular cholesterol transport Source: Ensembl
  6. positive regulation of viral release from host cell Source: Ensembl
  7. potassium ion transport Source: Ensembl
  8. protein transport Source: UniProtKB-KW
  9. response to lipid Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_212419. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 4B (EC:3.6.4.6)
Gene namesi
Name:VPS4B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 24

Subcellular locationi

Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane By similarity; Peripheral membrane protein By similarity
Note: Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body (By similarity).By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. early endosome Source: Ensembl
  3. endosome membrane Source: Ensembl
  4. extracellular vesicular exosome Source: Ensembl
  5. late endosome Source: Ensembl
  6. lysosome Source: Ensembl
  7. nucleus Source: Ensembl
  8. vacuolar membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Vacuolar protein sorting-associated protein 4BPRO_0000331378Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021PhosphoserineBy similarity
Modified residuei108 – 1081PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ0VD48.

Interactioni

Subunit structurei

Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP6. Interacts with VPS4B; the interaction suggests a heteromeric assembly with VPS4B. Interacts with SPAST. Interacts with IST1 (By similarity). with VTA1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013862.

Structurei

3D structure databases

ProteinModelPortaliQ0VD48.
SMRiQ0VD48. Positions 7-108, 123-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 8279MITAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili19 – 8264Sequence AnalysisAdd
BLAST

Domaini

The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2) (By similarity).By similarity

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated
Contains 1 MIT domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00550000074466.
HOGENOMiHOG000225146.
HOVERGENiHBG057074.
InParanoidiQ0VD48.
KOiK12196.
OMAiFRLYQHA.
OrthoDBiEOG74BJS2.
TreeFamiTF105012.

Family and domain databases

Gene3Di1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMiSSF116846. SSF116846. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0VD48-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASTPTNLQK AIDLASKAAQ EDKAGNYEEA LQLYQHAVQY FLHVVKYEAQ
60 70 80 90 100
GDKAKQSIRA KCTEYLDRAE KLKEYLKKRE KKPQKPVKEG QPAPADEKGN
110 120 130 140 150
DSDGEGESDD PEKKKLQNQL QGAIVIERPN VKWSDVAGLE GAKEALKEAV
160 170 180 190 200
ILPIKFPHLF TGKRTPWRGI LLFGPPGTGK SYLAKAVATE ANNSTFFSIS
210 220 230 240 250
SSDLVSKWLG ESEKLVKNLF QLARENKPSI IFIDEIDSLC GSRSENESEA
260 270 280 290 300
ARRIKTEFLV QMQGVGVDND GILVLGATNI PWVLDSAIRR RFEKRIYIPL
310 320 330 340 350
PEAHARAAMF KLHLGTTQNS LTEADFRDLG KKTEGYSGAD ISIIVRDALM
360 370 380 390 400
QPVRKVQSAT HFKKVRGPSR ADPNNIVDDL LTPCSPGDPG AIEMTWMDVP
410 420 430 440
GDKLLEPVVC MSDMLRSLSS TKPTVNEHDL LKLKKFTEDF GQEG
Length:444
Mass (Da):49,264
Last modified:September 5, 2006 - v1
Checksum:i7C9D31E0BE4C1861
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119836 mRNA. Translation: AAI19837.1.
RefSeqiNP_001069624.1. NM_001076156.1.
UniGeneiBt.62714.

Genome annotation databases

EnsembliENSBTAT00000013862; ENSBTAP00000013862; ENSBTAG00000010492.
GeneIDi539357.
KEGGibta:539357.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC119836 mRNA. Translation: AAI19837.1 .
RefSeqi NP_001069624.1. NM_001076156.1.
UniGenei Bt.62714.

3D structure databases

ProteinModelPortali Q0VD48.
SMRi Q0VD48. Positions 7-108, 123-444.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000013862.

Proteomic databases

PRIDEi Q0VD48.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000013862 ; ENSBTAP00000013862 ; ENSBTAG00000010492 .
GeneIDi 539357.
KEGGi bta:539357.

Organism-specific databases

CTDi 9525.

Phylogenomic databases

eggNOGi COG0464.
GeneTreei ENSGT00550000074466.
HOGENOMi HOG000225146.
HOVERGENi HBG057074.
InParanoidi Q0VD48.
KOi K12196.
OMAi FRLYQHA.
OrthoDBi EOG74BJS2.
TreeFami TF105012.

Enzyme and pathway databases

Reactomei REACT_212419. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

NextBioi 20877936.

Family and domain databases

Gene3Di 1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view ]
SUPFAMi SSF116846. SSF116846. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiVPS4B_BOVIN
AccessioniPrimary (citable) accession number: Q0VD48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: November 26, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3