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Q0VD48

- VPS4B_BOVIN

UniProt

Q0VD48 - VPS4B_BOVIN

Protein

Vacuolar protein sorting-associated protein 4B

Gene

VPS4B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (lentiviruses). Involved in cytokinesis By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi174 – 1818ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: Ensembl
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. endosome organization Source: Ensembl
    4. endosome to lysosome transport via multivesicular body sorting pathway Source: Ensembl
    5. intracellular cholesterol transport Source: Ensembl
    6. potassium ion transport Source: Ensembl
    7. protein transport Source: UniProtKB-KW
    8. response to lipid Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 4B (EC:3.6.4.6)
    Gene namesi
    Name:VPS4B
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 24

    Subcellular locationi

    Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane By similarity; Peripheral membrane protein By similarity
    Note: Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. early endosome Source: Ensembl
    3. late endosome membrane Source: UniProtKB-SubCell
    4. lysosome Source: Ensembl
    5. nucleus Source: Ensembl
    6. vacuolar membrane Source: Ensembl

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Vacuolar protein sorting-associated protein 4BPRO_0000331378Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei102 – 1021PhosphoserineBy similarity
    Modified residuei108 – 1081PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ0VD48.

    Interactioni

    Subunit structurei

    Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP6. Interacts with VPS4B; the interaction suggests a heteromeric assembly with VPS4B. Interacts with SPAST. Interacts with IST1 By similarity. with VTA1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000013862.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VD48.
    SMRiQ0VD48. Positions 7-108, 123-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 8279MITAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili19 – 8264Sequence AnalysisAdd
    BLAST

    Domaini

    The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2) By similarity.By similarity

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated
    Contains 1 MIT domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00550000074466.
    HOGENOMiHOG000225146.
    HOVERGENiHBG057074.
    InParanoidiQ0VD48.
    KOiK12196.
    OMAiFRLYQHA.
    OrthoDBiEOG74BJS2.
    TreeFamiTF105012.

    Family and domain databases

    Gene3Di1.20.58.280. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR007330. MIT.
    IPR027417. P-loop_NTPase.
    IPR015415. Vps4_C.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF04212. MIT. 1 hit.
    PF09336. Vps4_C. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00745. MIT. 1 hit.
    [Graphical view]
    SUPFAMiSSF116846. SSF116846. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0VD48-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTPTNLQK AIDLASKAAQ EDKAGNYEEA LQLYQHAVQY FLHVVKYEAQ    50
    GDKAKQSIRA KCTEYLDRAE KLKEYLKKRE KKPQKPVKEG QPAPADEKGN 100
    DSDGEGESDD PEKKKLQNQL QGAIVIERPN VKWSDVAGLE GAKEALKEAV 150
    ILPIKFPHLF TGKRTPWRGI LLFGPPGTGK SYLAKAVATE ANNSTFFSIS 200
    SSDLVSKWLG ESEKLVKNLF QLARENKPSI IFIDEIDSLC GSRSENESEA 250
    ARRIKTEFLV QMQGVGVDND GILVLGATNI PWVLDSAIRR RFEKRIYIPL 300
    PEAHARAAMF KLHLGTTQNS LTEADFRDLG KKTEGYSGAD ISIIVRDALM 350
    QPVRKVQSAT HFKKVRGPSR ADPNNIVDDL LTPCSPGDPG AIEMTWMDVP 400
    GDKLLEPVVC MSDMLRSLSS TKPTVNEHDL LKLKKFTEDF GQEG 444
    Length:444
    Mass (Da):49,264
    Last modified:September 5, 2006 - v1
    Checksum:i7C9D31E0BE4C1861
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC119836 mRNA. Translation: AAI19837.1.
    RefSeqiNP_001069624.1. NM_001076156.1.
    UniGeneiBt.62714.

    Genome annotation databases

    EnsembliENSBTAT00000013862; ENSBTAP00000013862; ENSBTAG00000010492.
    GeneIDi539357.
    KEGGibta:539357.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC119836 mRNA. Translation: AAI19837.1 .
    RefSeqi NP_001069624.1. NM_001076156.1.
    UniGenei Bt.62714.

    3D structure databases

    ProteinModelPortali Q0VD48.
    SMRi Q0VD48. Positions 7-108, 123-444.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000013862.

    Proteomic databases

    PRIDEi Q0VD48.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000013862 ; ENSBTAP00000013862 ; ENSBTAG00000010492 .
    GeneIDi 539357.
    KEGGi bta:539357.

    Organism-specific databases

    CTDi 9525.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00550000074466.
    HOGENOMi HOG000225146.
    HOVERGENi HBG057074.
    InParanoidi Q0VD48.
    KOi K12196.
    OMAi FRLYQHA.
    OrthoDBi EOG74BJS2.
    TreeFami TF105012.

    Miscellaneous databases

    NextBioi 20877936.

    Family and domain databases

    Gene3Di 1.20.58.280. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR007330. MIT.
    IPR027417. P-loop_NTPase.
    IPR015415. Vps4_C.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF04212. MIT. 1 hit.
    PF09336. Vps4_C. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00745. MIT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF116846. SSF116846. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Ascending colon.

    Entry informationi

    Entry nameiVPS4B_BOVIN
    AccessioniPrimary (citable) accession number: Q0VD48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3