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Q0VD48 (VPS4B_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 4B

EC=3.6.4.6
Gene names
Name:VPS4B
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (lentiviruses). Involved in cytokinesis By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP6. Interacts with VPS4B; the interaction suggests a heteromeric assembly with VPS4B. Interacts with SPAST. Interacts with IST1 By similarity. with VTA1 By similarity.

Subcellular location

Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein By similarity. Note: Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body By similarity.

Domain

The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2) By similarity.

Sequence similarities

Belongs to the AAA ATPase family.

Contains 1 MIT domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Protein transport
Transport
   Cellular componentEndosome
Membrane
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

endosome organization

Inferred from electronic annotation. Source: Ensembl

endosome to lysosome transport via multivesicular body sorting pathway

Inferred from electronic annotation. Source: Ensembl

intracellular cholesterol transport

Inferred from electronic annotation. Source: Ensembl

potassium ion transport

Inferred from electronic annotation. Source: Ensembl

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

response to lipid

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Inferred from electronic annotation. Source: Ensembl

early endosome

Inferred from electronic annotation. Source: Ensembl

late endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosome

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

vacuolar membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATPase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Vacuolar protein sorting-associated protein 4B
PRO_0000331378

Regions

Domain4 – 8279MIT
Nucleotide binding174 – 1818ATP Potential
Coiled coil19 – 8264 Potential

Amino acid modifications

Modified residue1021Phosphoserine By similarity
Modified residue1081Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0VD48 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 7C9D31E0BE4C1861

FASTA44449,264
        10         20         30         40         50         60 
MASTPTNLQK AIDLASKAAQ EDKAGNYEEA LQLYQHAVQY FLHVVKYEAQ GDKAKQSIRA 

        70         80         90        100        110        120 
KCTEYLDRAE KLKEYLKKRE KKPQKPVKEG QPAPADEKGN DSDGEGESDD PEKKKLQNQL 

       130        140        150        160        170        180 
QGAIVIERPN VKWSDVAGLE GAKEALKEAV ILPIKFPHLF TGKRTPWRGI LLFGPPGTGK 

       190        200        210        220        230        240 
SYLAKAVATE ANNSTFFSIS SSDLVSKWLG ESEKLVKNLF QLARENKPSI IFIDEIDSLC 

       250        260        270        280        290        300 
GSRSENESEA ARRIKTEFLV QMQGVGVDND GILVLGATNI PWVLDSAIRR RFEKRIYIPL 

       310        320        330        340        350        360 
PEAHARAAMF KLHLGTTQNS LTEADFRDLG KKTEGYSGAD ISIIVRDALM QPVRKVQSAT 

       370        380        390        400        410        420 
HFKKVRGPSR ADPNNIVDDL LTPCSPGDPG AIEMTWMDVP GDKLLEPVVC MSDMLRSLSS 

       430        440 
TKPTVNEHDL LKLKKFTEDF GQEG 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC119836 mRNA. Translation: AAI19837.1.
RefSeqNP_001069624.1. NM_001076156.1.
UniGeneBt.62714.

3D structure databases

ProteinModelPortalQ0VD48.
SMRQ0VD48. Positions 7-108, 123-444.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000013862.

Proteomic databases

PRIDEQ0VD48.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000013862; ENSBTAP00000013862; ENSBTAG00000010492.
GeneID539357.
KEGGbta:539357.

Organism-specific databases

CTD9525.

Phylogenomic databases

eggNOGCOG0464.
GeneTreeENSGT00550000074466.
HOGENOMHOG000225146.
HOVERGENHBG057074.
InParanoidQ0VD48.
KOK12196.
OMAFRLYQHA.
OrthoDBEOG74BJS2.
TreeFamTF105012.

Family and domain databases

Gene3D1.20.58.280. 1 hit.
3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
SUPFAMSSF116846. SSF116846. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20877936.

Entry information

Entry nameVPS4B_BOVIN
AccessionPrimary (citable) accession number: Q0VD48
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families