Reviewed, UniProtKB/Swiss-Prot Q0VD27 (ENOPH_BOVIN)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase
    MASA homolog

Gene names

Name:	ENOPH1
Synonyms:	MASA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Bifunctional enzyme that enolizes the substrate to form the intermediate 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate, which is then dephosphorylated to form the acireductone 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine salvage Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	acireductone synthase activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Enolase-phosphatase E1		PRO_0000254006
Regions
	Region	153 – 154	2	Substrate binding By similarity
	Compositional bias	48 – 51	4	Poly-Glu
Sites
	Metal binding	16	1	Magnesium By similarity
	Metal binding	18	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	212	1	Magnesium By similarity
	Binding site	187	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VD27-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D6288757291FAC10	FASTA	261	28,930
	10 20 30 40 50 60 MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYVKENVE EYLQAHWEEE ECQQDVRLLR 70 80 90 100 110 120 KQAEEDSHLD GAVPIPAASG NGADDPQWMI QAVVDNVYWQ MSLDRKTTAL KQLQGHMWRA 130 140 150 160 170 180 AFKAGHMKAE FFEDVVPAVR KWREAGMKVY VYSSGSVEAQ KLLFGHSTEG DILELVDGHF 190 200 210 220 230 240 DTKIGHKVES ESYQKIASSI GCSTNNILFL TDVSREASAA EEAGVHVAVV VRPGNAGLTD 250 260 DEKTHFSLIT SFSELYLPSS T

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thalamus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC119871 mRNA. Translation: AAI19872.1.
IPI	IPI00685778.
RefSeq	NP_001069343.1.
UniGene	Bt.3244
3D structure databases
SMR	Q0VD27. Positions 4-257.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VD27.
Genome annotation databases
Ensembl	ENSBTAT00000019702; ENSBTAP00000019702; ENSBTAG00000014805; Bos taurus. [Genome view]
GeneID	525563.
KEGG	bta:525563.
Organism-specific databases
CTD	525563.
Phylogenomic databases
HOVERGEN	Q0VD27.
OMA	AHWEEEE.
Enzyme and pathway databases
BRENDA	3.1.3.77. 251.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. IPR006439. HAD-SF_hydro_IA_v1. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit. TIGR01549. HAD-SF-IA-v1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENOPH_BOVIN

Accession

Primary (citable) accession number: Q0VD27

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)