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Q0VCX2 (GRP78_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
78 kDa glucose-regulated protein

Short name=GRP-78
Alternative name(s):
Heat shock 70 kDa protein 5
Gene names
Name:HSPA5
Synonyms:GRP78
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate By similarity.

Subunit structure

Interacts with DNAJC1 (via J domain) By similarity. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A and TRIM21. May form a complex with ERLEC1, OS9, SEL1L and SYVN1 By similarity. Interacts with MX1. Interacts with METTL23 and DNAJC10. Interacts with CEMIP; the interaction induces calcium leakage from the endoplamic reticulum and cell migration By similarity.

Subcellular location

Endoplasmic reticulum lumen By similarity. Melanosome By similarity. Cytoplasm By similarity.

Sequence similarities

Belongs to the heat shock protein 70 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Chain20 – 65563678 kDa glucose-regulated protein
PRO_0000288774

Regions

Nucleotide binding37 – 404ATP By similarity
Nucleotide binding228 – 2303ATP By similarity
Nucleotide binding294 – 3018ATP By similarity
Nucleotide binding365 – 3684ATP By similarity
Motif652 – 6554Prevents secretion from ER By similarity

Sites

Binding site971ATP By similarity

Amino acid modifications

Modified residue1261N6-acetyllysine By similarity
Modified residue1611Nitrated tyrosine By similarity
Modified residue2141N6-acetyllysine By similarity
Modified residue3271N6-acetyllysine By similarity
Modified residue3541N6-acetyllysine By similarity
Modified residue4481N6-succinyllysine By similarity
Modified residue5191Phosphothreonine By similarity
Modified residue5861N6,N6,N6-trimethyllysine; by METTL21A; in vitro By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0VCX2 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 93B4C3D46A441066

FASTA65572,400
        10         20         30         40         50         60 
MKLSLVAAVL LLLLGTARAE EEDKKEDVGT VVGIDLGTTY SCVGVFKNGR VEIIANDQGN 

        70         80         90        100        110        120 
RITPSYVAFT PEGERLIGDA AKNQLTSNPE NTVFDAKRLI GRTWNDPSVQ QDIKFLPFKV 

       130        140        150        160        170        180 
VEKKTKPYIQ VDVGGGQTKT FAPEEISAMV LTKMKETAEA YLGKKVTHAV VTVPAYFNDA 

       190        200        210        220        230        240 
QRQATKDAGT IAGLNVMRII NEPTAAAIAY GLDKREGEKN ILVFDLGGGT FDVSLLTIDN 

       250        260        270        280        290        300 
GVFEVVATNG DTHLGGEDFD QRVMEHFIKL YKKKTGKDVR KDNRAVQKLR REVEKAKRAL 

       310        320        330        340        350        360 
SSQHQARIEI ESFYEGEDFS ETLTRAKFEE LNMDLFRSTM KPVQKVLEDS DLKKSDIDEI 

       370        380        390        400        410        420 
VLVGGSTRIP KIQQLVKEFF NGKEPSRGIN PDEAVAYGAA VQAGVLSGDQ DTGDLVLLDV 

       430        440        450        460        470        480 
CPLTLGIETV GGVMTKLIPR NTVVPTKKSQ IFSTASDNQP TVTIKVYEGE RPLTKDNHLL 

       490        500        510        520        530        540 
GTFDLTGIPP APRGVPQIEV TFEIDVNGIL RVTAEDKGTG NKNKITITND QNRLTPEEIE 

       550        560        570        580        590        600 
RMVNDAEKFA EEDKKLKERI DTRNELESYA YSLKNQIGDK EKLGGKLSSE DKETMEKAVE 

       610        620        630        640        650 
EKIEWLESHQ DADIEDFKAK KKELEEIVQP IISKLYGSAG PPPTSEEEAA DKDEL 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal skin.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT030726 mRNA. Translation: ABS45042.1.
BC119953 mRNA. Translation: AAI19954.1.
RefSeqNP_001068616.1. NM_001075148.1.
UniGeneBt.65104.

3D structure databases

ProteinModelPortalQ0VCX2.
SMRQ0VCX2. Positions 29-571.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60411N.
IntActQ0VCX2. 1 interaction.

Proteomic databases

PRIDEQ0VCX2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID415113.
KEGGbta:415113.

Organism-specific databases

CTD3309.

Phylogenomic databases

HOVERGENHBG051845.
KOK09490.

Family and domain databases

Gene3D1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
SUPFAMSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20818797.
PROQ0VCX2.

Entry information

Entry nameGRP78_BOVIN
AccessionPrimary (citable) accession number: Q0VCX2
Secondary accession number(s): A7E3V5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: September 5, 2006
Last modified: June 11, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families