Complement C1s subcomponent precursor

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Reviewed, UniProtKB/Swiss-Prot Q0VCX1 (C1S_BOVIN)

Last modified June 16, 2009. Version 29. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Complement C1s subcomponent
    EC=3.4.21.42
Alternative name(s):
    C1 esterase
Cleaved into the following 2 chains:
    1- Recommended name:
            Complement C1s subcomponent heavy chain
    2- Recommended name:
            Complement C1s subcomponent light chain

Gene names

Name:

C1S

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4 By similarity.
Catalytic activity	Cleavage of Arg-\|-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg)-\|-Lys bond in complement component C2 to form C2a and C2b: the 'classical' pathway C3 convertase.
Enzyme regulation	Inhibited by SERPING1 By similarity.
Subunit structure	C1 is a calcium-dependent trimolecular complex of C1q, C1r and C1s in the molar ration of 1:2:2. Activated C1s is an disulfide-linked heterodimer of a heavy chain and a light chain By similarity.
Post-translational modification	The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 2 CUB domains. Contains 1 EGF-like domain. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains.

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Ontologies

Keywords
Biological process	Complement pathway Immune response Innate immunity
Domain	EGF-like domain Repeat Signal Sushi
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Hydroxylation
Gene Ontology (GO)
Biological process	complement activation, classical pathway Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 15

Potential

Chain

16 – 689

674

Complement C1s subcomponent

PRO_0000285867

Chain

16 – 437

422

Complement C1s subcomponent heavy chain By similarity

PRO_0000285868

Chain

438 – 689

252

Complement C1s subcomponent light chain By similarity

PRO_0000285869

Regions

Domain

16 – 130

115

CUB 1

Domain

131 – 172

EGF-like; calcium-binding Potential

Domain

175 – 290

116

CUB 2

Domain

292 – 356

Sushi 1

Domain

357 – 423

Sushi 2

Domain

438 – 681

244

Peptidase S1

Sites

Active site

475

Charge relay system By similarity

Active site

529

Charge relay system By similarity

Active site

632

Charge relay system By similarity

Metal binding

Calcium By similarity

Metal binding

Calcium By similarity

Metal binding

113

Calcium By similarity

Metal binding

131

Calcium By similarity

Metal binding

132

Calcium; via carbonyl oxygen By similarity

Metal binding

134

Calcium By similarity

Metal binding

149

Calcium By similarity

Metal binding

150

Calcium; via carbonyl oxygen By similarity

Metal binding

153

Calcium; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue

149

(3R)-3-hydroxyasparagine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

174

N-linked (GlcNAc...) Potential

Glycosylation

406

N-linked (GlcNAc...) Potential

Disulfide bond

65 ↔ 83

By similarity

Disulfide bond

135 ↔ 147

By similarity

Disulfide bond

143 ↔ 156

By similarity

Disulfide bond

158 ↔ 171

By similarity

Disulfide bond

175 ↔ 202

By similarity

Disulfide bond

234 ↔ 251

By similarity

Disulfide bond

294 ↔ 341

By similarity

Disulfide bond

321 ↔ 354

By similarity

Disulfide bond

359 ↔ 403

By similarity

Disulfide bond

386 ↔ 421

By similarity

Disulfide bond

425 ↔ 549

Interchain (between heavy and light chains) By similarity

Disulfide bond

595 ↔ 618

By similarity

Disulfide bond

628 ↔ 660

By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q0VCX1-1 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: B10A00EC4B15465C
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FASTA

689

76,609

        10         20         30         40         50         60 
MWCIVLFSLV AWVYAEPTMY GEILSPNYPQ VYPNEVEKSW DIEVPAGYGI HLYFTHLDIE 

        70         80         90        100        110        120 
LSENCSYDSV QIMSGGHEEG KLCGRRTNKN SNSPVVKEFH IPYSKLQVIF RSDFSNEERF 

       130        140        150        160        170        180 
TGFAAYYVAE DIDECTAFAD APCSHFCNNF LGGYFCSCPP EYFLHEDKKN CGVNCSGNVF 

       190        200        210        220        230        240 
TTMTGEVESP NYPSPYPESS RCDYQIQLEE GFRVVVTMRR EDFDVEPADS EGHCPDSLLF 

       250        260        270        280        290        300 
VAGDQHFGPY CGNGFPGPLT IETQSSALNI IFQTDGSEQR KGWKFRYHGD PIPCPKEVTA 

       310        320        330        340        350        360 
NSFWEPERAK YVFRDVVKIT CVDGFEVVQG SVGSPSFYST CQSNGKWSNS KLRCQPVDCG 

       370        380        390        400        410        420 
APEPIQHGRV EDPESTLFGS ITRYSCEMPY YSMECEGSEV YHCSGNGSWV NKVLGIEPPK 

       430        440        450        460        470        480 
CIAVCGTPSE PFRSTQRIFG GSIAKIENFP WQVFFSNPWA GGALIDEYWV LTAAHVVEGN 

       490        500        510        520        530        540 
DIPVMYVGSS SVVTSQLSNA QMLTAERVFI HPGWEVLDPS ITRKNFDNDI ALVRLRDPVK 

       550        560        570        580        590        600 
MGPKVAPICL PGTSSEYDPP ENVLGLISGW GRTNVKSHVI KLRGAKLPVA PLSKCREMKG 

       610        620        630        640        650        660 
VNPGIDISSF VFTENMICAG NDKGVDSCDG DSGGAFAVQD PKENKPKFYV AGLVSWGPQC 

       670        680 
GTYGIYTRVK NYVDWIRKTM QEYSAPSVD

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References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal skin.

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Cross-references

Sequence databases
	BC119956 mRNA. Translation: AAI19957.1. Different initiation.
IPI	IPI00695404.
RefSeq	NP_001070018.1.
UniGene	Bt.1491
3D structure databases
SMR	Q0VCX1. Positions 18-174, 357-684.
ModBase	Search...
Protein family/group databases
MEROPS	S01.193.
Genome annotation databases
Ensembl	ENSBTAG00000004840. Bos taurus. [Contig view]
GeneID	767827.
KEGG	bta:767827.
Phylogenomic databases
HOVERGEN	Q0VCX1.
Enzyme and pathway databases
BRENDA	3.4.21.42. 251.
Family and domain databases
InterPro	IPR016060. Complement_control_module. IPR000859. CUB. IPR006209. EGF. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_CS. IPR001881. EGF_Ca_bd. IPR018097. EGF_Ca_bd_CS. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR000436. Sushi_SCR_CCP. [Graphical view]
Gene3D	G3DSA:2.10.70.10. Complement_control_module. 1 hit. G3DSA:2.60.120.290. CUB. 2 hits.
Pfam	PF00431. CUB. 2 hits. PF00008. EGF. 1 hit. PF00084. Sushi. 2 hits. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00032. CCP. 2 hits. SM00042. CUB. 2 hits. SM00179. EGF_CA. 1 hit. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS01180. CUB. 2 hits. PS00022. EGF_1. False negative. PS01186. EGF_2. False negative. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. PS50923. SUSHI. 2 hits. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. False negative. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

C1S_BOVIN

Accession

Primary (citable) accession number: Q0VCX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents