Reviewed, UniProtKB/Swiss-Prot Q0VCR7 (C1TM_BOVIN)

Names and origin

Protein names

Recommended name:
    Monofunctional C1-tetrahydrofolate synthase, mitochondrial
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase

Gene names

Name:

MTHFD1L

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	975 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2 By similarity.
Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion By similarity.
Domain	This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.
Sequence similarities	In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family. In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion By similarity
	Chain	31 – 975	945	Monofunctional C1-tetrahydrofolate synthase, mitochondrial		PRO_0000343176
Regions
	Nucleotide binding	420 – 427	8	ATP By similarity
	Region	31 – 345	315	Methylenetetrahydrofolate dehydrogenase and cyclohydrolase
	Region	346 – 975	630	Formyltetrahydrofolate synthetase
	Compositional bias	33 – 41	9	Poly-Gly
Amino acid modifications
	Modified residue	172	1	N6-acetyllysine By similarity
	Modified residue	187	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	195	1	E → G in AAI20039. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q0VCR7-1 [UniParc]. Last modified July 1, 2008. Version 2. Checksum: 4B7D0B35164A580B	FASTA	975	105,227
	10 20 30 40 50 60 MSARLPFVLR RLARPQHPGS PRRLPSLCRA SSGRGSGCGG GEGLLGQQRL RDTQAGSSRG 70 80 90 100 110 120 PGSPAPPARD SIVREVIQNS KEVLSLLQEK TPTFKPVLAI IQAGDDNLMQ EVNQNLAEEA 130 140 150 160 170 180 GLNITHICLP AESGEDEIID EILKINEDSR VHGLALQIAE TSFSNKILNA LKPEKDVDGL 190 200 210 220 230 240 TDVNLGKLVR GDAHECFISP VARAVIELLE KSGVSLDGKK ILVIGAHGSL EATLQCLFQR 250 260 270 280 290 300 KGSMTMSSQW KTPQLQGKLQ EADIVVLGSP KPEEIPLSWI QPGTTVFNCS HDFLSGKAAC 310 320 330 340 350 360 ISSGVHGISP IAEDVSLLAA ALRIQNMVSS GRRWLREQQH RRWRLHCLKL QPLSPVPSDI 370 380 390 400 410 420 EISRAQTPKA VEILAKEIGL LADEIEIYGK SKAKVRLSLL ERLKDQADGK YVLVAGITPT 430 440 450 460 470 480 PLGEGKSTVT IGLVQALTAH LNVNSFACLR QPSQGPTFGV KGGAAGGGYA QVIPMEEFNL 490 500 510 520 530 540 HLTGDIHAIT AANNLLAAAI DARILHENTQ TDKALYNRLV PSVNGVREFS KIQLARLKRL 550 560 570 580 590 600 GINKTDPSAL TEEEMRKFAR LDIDPSTITW QRVVDTNDRF LRKITIGQAN TEKGCSRQAQ 610 620 630 640 650 660 FDIAVASEIM AVLALTDSLS DMKERLGRMV VASDRNGQPV TADDLGVTGA LTVLMKDAIK 670 680 690 700 710 720 PNLMQTLEGT PVFVHAGPFA NIAHGNSSVL ADKIALKLVG EGGFVVTEAG FGADIGMEKF 730 740 750 760 770 780 FNIKCRASGL VPSVVVLVAT VRALKMHGGG PSVTAGVPLR KEYTEENLQL VADGCCNLEK 790 800 810 820 830 840 QIQIAQLFGV PVVVALNVFK TDTRAEIDLV CELAKRAGAF NAVPCYHWSI GGKGSVDLAW 850 860 870 880 890 900 AVREAASKES RFQFLYDVQL PIVEKIRTIA QSVYGAKDIE LSPEAQSKID RYTEQGFGNL 910 920 930 940 950 960 PICMAKTHLS LSHQPDKKGV PKGFILPISD VRASIGAGFI YPLVGTMSTM PGLPTRPCFY 970 DIDLDTETEQ VKGLF

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver and Thymus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC120038 mRNA. Translation: AAI20039.1. BC148878 mRNA. Translation: AAI48879.1.
IPI	IPI00711967.
RefSeq	NP_001069486.1.
UniGene	Bt.7490
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0VCR7.
Genome annotation databases
Ensembl	ENSBTAT00000012982; ENSBTAP00000012982; ENSBTAG00000009846; Bos taurus. [Genome view]
GeneID	534296.
KEGG	bta:534296.
Organism-specific databases
CTD	534296.
Phylogenomic databases
HOVERGEN	Q0VCR7.
OMA	NMVSSGR.
Family and domain databases
InterPro	IPR000559. For_THF_ligase. IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01268. FTHFS. 1 hit. PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProDom	PD002300. THFDhg/Cyc_hydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C1TM_BOVIN

Accession

Primary (citable) accession number: Q0VCR7
Secondary accession number(s): A6QNK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	November 3, 2009
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)