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Q0VCR7

- C1TM_BOVIN

UniProt

Q0VCR7 - C1TM_BOVIN

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Protein

Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Gene

MTHFD1L

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.By similarity

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi420 – 4278ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. formate-tetrahydrofolate ligase activity Source: RefGenome

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. one-carbon metabolic process Source: RefGenome
  3. oxidation-reduction process Source: InterPro
  4. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Monofunctional C1-tetrahydrofolate synthase, mitochondrial (EC:6.3.4.3)
Alternative name(s):
Formyltetrahydrofolate synthetase
Gene namesi
Name:MTHFD1L
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionBy similarityAdd
BLAST
Chaini31 – 975945Monofunctional C1-tetrahydrofolate synthase, mitochondrialPRO_0000343176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871N6-acetyllysine; alternateBy similarity
Modified residuei187 – 1871N6-succinyllysine; alternateBy similarity
Modified residuei593 – 5931N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ0VCR7.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ0VCR7.
SMRiQ0VCR7. Positions 509-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 345315Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd
BLAST
Regioni346 – 975630Formyltetrahydrofolate synthetaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi33 – 419Poly-Gly

Domaini

This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0190.
HOGENOMiHOG000040280.
HOVERGENiHBG004916.
InParanoidiQ0VCR7.
KOiK13402.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0VCR7-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSARLPFVLR RLARPQHPGS PRRLPSLCRA SSGRGSGCGG GEGLLGQQRL
60 70 80 90 100
RDTQAGSSRG PGSPAPPARD SIVREVIQNS KEVLSLLQEK TPTFKPVLAI
110 120 130 140 150
IQAGDDNLMQ EVNQNLAEEA GLNITHICLP AESGEDEIID EILKINEDSR
160 170 180 190 200
VHGLALQIAE TSFSNKILNA LKPEKDVDGL TDVNLGKLVR GDAHECFISP
210 220 230 240 250
VARAVIELLE KSGVSLDGKK ILVIGAHGSL EATLQCLFQR KGSMTMSSQW
260 270 280 290 300
KTPQLQGKLQ EADIVVLGSP KPEEIPLSWI QPGTTVFNCS HDFLSGKAAC
310 320 330 340 350
ISSGVHGISP IAEDVSLLAA ALRIQNMVSS GRRWLREQQH RRWRLHCLKL
360 370 380 390 400
QPLSPVPSDI EISRAQTPKA VEILAKEIGL LADEIEIYGK SKAKVRLSLL
410 420 430 440 450
ERLKDQADGK YVLVAGITPT PLGEGKSTVT IGLVQALTAH LNVNSFACLR
460 470 480 490 500
QPSQGPTFGV KGGAAGGGYA QVIPMEEFNL HLTGDIHAIT AANNLLAAAI
510 520 530 540 550
DARILHENTQ TDKALYNRLV PSVNGVREFS KIQLARLKRL GINKTDPSAL
560 570 580 590 600
TEEEMRKFAR LDIDPSTITW QRVVDTNDRF LRKITIGQAN TEKGCSRQAQ
610 620 630 640 650
FDIAVASEIM AVLALTDSLS DMKERLGRMV VASDRNGQPV TADDLGVTGA
660 670 680 690 700
LTVLMKDAIK PNLMQTLEGT PVFVHAGPFA NIAHGNSSVL ADKIALKLVG
710 720 730 740 750
EGGFVVTEAG FGADIGMEKF FNIKCRASGL VPSVVVLVAT VRALKMHGGG
760 770 780 790 800
PSVTAGVPLR KEYTEENLQL VADGCCNLEK QIQIAQLFGV PVVVALNVFK
810 820 830 840 850
TDTRAEIDLV CELAKRAGAF NAVPCYHWSI GGKGSVDLAW AVREAASKES
860 870 880 890 900
RFQFLYDVQL PIVEKIRTIA QSVYGAKDIE LSPEAQSKID RYTEQGFGNL
910 920 930 940 950
PICMAKTHLS LSHQPDKKGV PKGFILPISD VRASIGAGFI YPLVGTMSTM
960 970
PGLPTRPCFY DIDLDTETEQ VKGLF
Length:975
Mass (Da):105,227
Last modified:July 1, 2008 - v2
Checksum:i4B7D0B35164A580B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951E → G in AAI20039. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120038 mRNA. Translation: AAI20039.1.
BC148878 mRNA. Translation: AAI48879.1.
RefSeqiNP_001069486.1. NM_001076018.2.
UniGeneiBt.7490.

Genome annotation databases

GeneIDi534296.
KEGGibta:534296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120038 mRNA. Translation: AAI20039.1 .
BC148878 mRNA. Translation: AAI48879.1 .
RefSeqi NP_001069486.1. NM_001076018.2.
UniGenei Bt.7490.

3D structure databases

ProteinModelPortali Q0VCR7.
SMRi Q0VCR7. Positions 509-571.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q0VCR7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 534296.
KEGGi bta:534296.

Organism-specific databases

CTDi 25902.

Phylogenomic databases

eggNOGi COG0190.
HOGENOMi HOG000040280.
HOVERGENi HBG004916.
InParanoidi Q0VCR7.
KOi K13402.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Miscellaneous databases

NextBioi 20876344.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
3.40.50.720. 2 hits.
HAMAPi MF_01543. FTHFS.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view ]
PRINTSi PR00085. THFDHDRGNASE.
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver and Thymus.

Entry informationi

Entry nameiC1TM_BOVIN
AccessioniPrimary (citable) accession number: Q0VCR7
Secondary accession number(s): A6QNK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: October 1, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3