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Q0VCR7

- C1TM_BOVIN

UniProt

Q0VCR7 - C1TM_BOVIN

Protein

Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Gene

MTHFD1L

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.By similarity

    Catalytic activityi

    ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi420 – 4278ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. formate-tetrahydrofolate ligase activity Source: RefGenome

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. one-carbon metabolic process Source: RefGenome
    3. oxidation-reduction process Source: InterPro
    4. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Monofunctional C1-tetrahydrofolate synthase, mitochondrial (EC:6.3.4.3)
    Alternative name(s):
    Formyltetrahydrofolate synthetase
    Gene namesi
    Name:MTHFD1L
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: RefGenome

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionBy similarityAdd
    BLAST
    Chaini31 – 975945Monofunctional C1-tetrahydrofolate synthase, mitochondrialPRO_0000343176Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei187 – 1871N6-acetyllysine; alternateBy similarity
    Modified residuei187 – 1871N6-succinyllysine; alternateBy similarity
    Modified residuei593 – 5931N6-succinyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ0VCR7.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VCR7.
    SMRiQ0VCR7. Positions 509-571.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni31 – 345315Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd
    BLAST
    Regioni346 – 975630Formyltetrahydrofolate synthetaseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi33 – 419Poly-Gly

    Domaini

    This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.

    Sequence similaritiesi

    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0190.
    HOGENOMiHOG000040280.
    HOVERGENiHBG004916.
    InParanoidiQ0VCR7.
    KOiK13402.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    3.40.50.720. 2 hits.
    HAMAPiMF_01543. FTHFS.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR000672. THF_DH/CycHdrlase.
    IPR020630. THF_DH/CycHdrlase_cat_dom.
    IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    PF00763. THF_DHG_CYH. 1 hit.
    PF02882. THF_DHG_CYH_C. 1 hit.
    [Graphical view]
    PRINTSiPR00085. THFDHDRGNASE.
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0VCR7-1 [UniParc]FASTAAdd to Basket

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    MSARLPFVLR RLARPQHPGS PRRLPSLCRA SSGRGSGCGG GEGLLGQQRL    50
    RDTQAGSSRG PGSPAPPARD SIVREVIQNS KEVLSLLQEK TPTFKPVLAI 100
    IQAGDDNLMQ EVNQNLAEEA GLNITHICLP AESGEDEIID EILKINEDSR 150
    VHGLALQIAE TSFSNKILNA LKPEKDVDGL TDVNLGKLVR GDAHECFISP 200
    VARAVIELLE KSGVSLDGKK ILVIGAHGSL EATLQCLFQR KGSMTMSSQW 250
    KTPQLQGKLQ EADIVVLGSP KPEEIPLSWI QPGTTVFNCS HDFLSGKAAC 300
    ISSGVHGISP IAEDVSLLAA ALRIQNMVSS GRRWLREQQH RRWRLHCLKL 350
    QPLSPVPSDI EISRAQTPKA VEILAKEIGL LADEIEIYGK SKAKVRLSLL 400
    ERLKDQADGK YVLVAGITPT PLGEGKSTVT IGLVQALTAH LNVNSFACLR 450
    QPSQGPTFGV KGGAAGGGYA QVIPMEEFNL HLTGDIHAIT AANNLLAAAI 500
    DARILHENTQ TDKALYNRLV PSVNGVREFS KIQLARLKRL GINKTDPSAL 550
    TEEEMRKFAR LDIDPSTITW QRVVDTNDRF LRKITIGQAN TEKGCSRQAQ 600
    FDIAVASEIM AVLALTDSLS DMKERLGRMV VASDRNGQPV TADDLGVTGA 650
    LTVLMKDAIK PNLMQTLEGT PVFVHAGPFA NIAHGNSSVL ADKIALKLVG 700
    EGGFVVTEAG FGADIGMEKF FNIKCRASGL VPSVVVLVAT VRALKMHGGG 750
    PSVTAGVPLR KEYTEENLQL VADGCCNLEK QIQIAQLFGV PVVVALNVFK 800
    TDTRAEIDLV CELAKRAGAF NAVPCYHWSI GGKGSVDLAW AVREAASKES 850
    RFQFLYDVQL PIVEKIRTIA QSVYGAKDIE LSPEAQSKID RYTEQGFGNL 900
    PICMAKTHLS LSHQPDKKGV PKGFILPISD VRASIGAGFI YPLVGTMSTM 950
    PGLPTRPCFY DIDLDTETEQ VKGLF 975
    Length:975
    Mass (Da):105,227
    Last modified:July 1, 2008 - v2
    Checksum:i4B7D0B35164A580B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti195 – 1951E → G in AAI20039. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120038 mRNA. Translation: AAI20039.1.
    BC148878 mRNA. Translation: AAI48879.1.
    RefSeqiNP_001069486.1. NM_001076018.2.
    UniGeneiBt.7490.

    Genome annotation databases

    GeneIDi534296.
    KEGGibta:534296.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120038 mRNA. Translation: AAI20039.1 .
    BC148878 mRNA. Translation: AAI48879.1 .
    RefSeqi NP_001069486.1. NM_001076018.2.
    UniGenei Bt.7490.

    3D structure databases

    ProteinModelPortali Q0VCR7.
    SMRi Q0VCR7. Positions 509-571.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q0VCR7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 534296.
    KEGGi bta:534296.

    Organism-specific databases

    CTDi 25902.

    Phylogenomic databases

    eggNOGi COG0190.
    HOGENOMi HOG000040280.
    HOVERGENi HBG004916.
    InParanoidi Q0VCR7.
    KOi K13402.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Miscellaneous databases

    NextBioi 20876344.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    3.40.50.720. 2 hits.
    HAMAPi MF_01543. FTHFS.
    InterProi IPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR000672. THF_DH/CycHdrlase.
    IPR020630. THF_DH/CycHdrlase_cat_dom.
    IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF01268. FTHFS. 1 hit.
    PF00763. THF_DHG_CYH. 1 hit.
    PF02882. THF_DHG_CYH_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00085. THFDHDRGNASE.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal liver and Thymus.

    Entry informationi

    Entry nameiC1TM_BOVIN
    AccessioniPrimary (citable) accession number: Q0VCR7
    Secondary accession number(s): A6QNK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3