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Protein

Monofunctional C1-tetrahydrofolate synthase, mitochondrial

Gene

MTHFD1L

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.By similarity

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi420 – 427ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Monofunctional C1-tetrahydrofolate synthase, mitochondrial (EC:6.3.4.3)
Alternative name(s):
Formyltetrahydrofolate synthetase
Gene namesi
Name:MTHFD1L
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionBy similarityAdd BLAST30
ChainiPRO_000034317631 – 975Monofunctional C1-tetrahydrofolate synthase, mitochondrialAdd BLAST945

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187N6-acetyllysine; alternateBy similarity1
Modified residuei187N6-succinyllysine; alternateBy similarity1
Modified residuei354PhosphoserineBy similarity1
Modified residuei593N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ0VCR7.
PeptideAtlasiQ0VCR7.
PRIDEiQ0VCR7.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000052260.

Structurei

3D structure databases

ProteinModelPortaliQ0VCR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 345Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseAdd BLAST315
Regioni346 – 975Formyltetrahydrofolate synthetaseAdd BLAST630

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 41Poly-Gly9

Domaini

This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.Curated
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4230. Eukaryota.
COG0190. LUCA.
COG2759. LUCA.
HOGENOMiHOG000040280.
HOVERGENiHBG004916.
InParanoidiQ0VCR7.
KOiK13402.

Family and domain databases

CDDicd00477. FTHFS. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 2 hits.
HAMAPiMF_01543. FTHFS. 1 hit.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0VCR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARLPFVLR RLARPQHPGS PRRLPSLCRA SSGRGSGCGG GEGLLGQQRL
60 70 80 90 100
RDTQAGSSRG PGSPAPPARD SIVREVIQNS KEVLSLLQEK TPTFKPVLAI
110 120 130 140 150
IQAGDDNLMQ EVNQNLAEEA GLNITHICLP AESGEDEIID EILKINEDSR
160 170 180 190 200
VHGLALQIAE TSFSNKILNA LKPEKDVDGL TDVNLGKLVR GDAHECFISP
210 220 230 240 250
VARAVIELLE KSGVSLDGKK ILVIGAHGSL EATLQCLFQR KGSMTMSSQW
260 270 280 290 300
KTPQLQGKLQ EADIVVLGSP KPEEIPLSWI QPGTTVFNCS HDFLSGKAAC
310 320 330 340 350
ISSGVHGISP IAEDVSLLAA ALRIQNMVSS GRRWLREQQH RRWRLHCLKL
360 370 380 390 400
QPLSPVPSDI EISRAQTPKA VEILAKEIGL LADEIEIYGK SKAKVRLSLL
410 420 430 440 450
ERLKDQADGK YVLVAGITPT PLGEGKSTVT IGLVQALTAH LNVNSFACLR
460 470 480 490 500
QPSQGPTFGV KGGAAGGGYA QVIPMEEFNL HLTGDIHAIT AANNLLAAAI
510 520 530 540 550
DARILHENTQ TDKALYNRLV PSVNGVREFS KIQLARLKRL GINKTDPSAL
560 570 580 590 600
TEEEMRKFAR LDIDPSTITW QRVVDTNDRF LRKITIGQAN TEKGCSRQAQ
610 620 630 640 650
FDIAVASEIM AVLALTDSLS DMKERLGRMV VASDRNGQPV TADDLGVTGA
660 670 680 690 700
LTVLMKDAIK PNLMQTLEGT PVFVHAGPFA NIAHGNSSVL ADKIALKLVG
710 720 730 740 750
EGGFVVTEAG FGADIGMEKF FNIKCRASGL VPSVVVLVAT VRALKMHGGG
760 770 780 790 800
PSVTAGVPLR KEYTEENLQL VADGCCNLEK QIQIAQLFGV PVVVALNVFK
810 820 830 840 850
TDTRAEIDLV CELAKRAGAF NAVPCYHWSI GGKGSVDLAW AVREAASKES
860 870 880 890 900
RFQFLYDVQL PIVEKIRTIA QSVYGAKDIE LSPEAQSKID RYTEQGFGNL
910 920 930 940 950
PICMAKTHLS LSHQPDKKGV PKGFILPISD VRASIGAGFI YPLVGTMSTM
960 970
PGLPTRPCFY DIDLDTETEQ VKGLF
Length:975
Mass (Da):105,227
Last modified:July 1, 2008 - v2
Checksum:i4B7D0B35164A580B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195E → G in AAI20039 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120038 mRNA. Translation: AAI20039.1.
BC148878 mRNA. Translation: AAI48879.1.
RefSeqiNP_001069486.1. NM_001076018.2.
UniGeneiBt.7490.

Genome annotation databases

GeneIDi534296.
KEGGibta:534296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120038 mRNA. Translation: AAI20039.1.
BC148878 mRNA. Translation: AAI48879.1.
RefSeqiNP_001069486.1. NM_001076018.2.
UniGeneiBt.7490.

3D structure databases

ProteinModelPortaliQ0VCR7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000052260.

Proteomic databases

PaxDbiQ0VCR7.
PeptideAtlasiQ0VCR7.
PRIDEiQ0VCR7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi534296.
KEGGibta:534296.

Organism-specific databases

CTDi25902.

Phylogenomic databases

eggNOGiKOG4230. Eukaryota.
COG0190. LUCA.
COG2759. LUCA.
HOGENOMiHOG000040280.
HOVERGENiHBG004916.
InParanoidiQ0VCR7.
KOiK13402.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

CDDicd00477. FTHFS. 1 hit.
Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 2 hits.
HAMAPiMF_01543. FTHFS. 1 hit.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC1TM_BOVIN
AccessioniPrimary (citable) accession number: Q0VCR7
Secondary accession number(s): A6QNK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: October 5, 2016
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.