SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q0VCR7

- C1TM_BOVIN

UniProt

Q0VCR7 - C1TM_BOVIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Monofunctional C1-tetrahydrofolate synthase, mitochondrial
Gene
MTHFD1L
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2 By similarity.UniRule annotation

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi420 – 4278ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. formate-tetrahydrofolate ligase activity Source: RefGenome

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. one-carbon metabolic process Source: RefGenome
  3. oxidation-reduction process Source: InterPro
  4. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Monofunctional C1-tetrahydrofolate synthase, mitochondrial (EC:6.3.4.3)
Alternative name(s):
Formyltetrahydrofolate synthetase
Gene namesi
Name:MTHFD1L
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030Mitochondrion By similarity
Add
BLAST
Chaini31 – 975945Monofunctional C1-tetrahydrofolate synthase, mitochondrialUniRule annotation
PRO_0000343176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871N6-acetyllysine; alternate By similarity
Modified residuei187 – 1871N6-succinyllysine; alternate By similarity
Modified residuei593 – 5931N6-succinyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ0VCR7.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ0VCR7.
SMRiQ0VCR7. Positions 509-571.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 345315Methylenetetrahydrofolate dehydrogenase and cyclohydrolaseUniRule annotation
Add
BLAST
Regioni346 – 975630Formyltetrahydrofolate synthetaseUniRule annotation
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi33 – 419Poly-GlyUniRule annotation

Domaini

This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0190.
HOGENOMiHOG000040280.
HOVERGENiHBG004916.
InParanoidiQ0VCR7.
KOiK13402.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
3.40.50.720. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0VCR7-1 [UniParc]FASTAAdd to Basket

« Hide

MSARLPFVLR RLARPQHPGS PRRLPSLCRA SSGRGSGCGG GEGLLGQQRL    50
RDTQAGSSRG PGSPAPPARD SIVREVIQNS KEVLSLLQEK TPTFKPVLAI 100
IQAGDDNLMQ EVNQNLAEEA GLNITHICLP AESGEDEIID EILKINEDSR 150
VHGLALQIAE TSFSNKILNA LKPEKDVDGL TDVNLGKLVR GDAHECFISP 200
VARAVIELLE KSGVSLDGKK ILVIGAHGSL EATLQCLFQR KGSMTMSSQW 250
KTPQLQGKLQ EADIVVLGSP KPEEIPLSWI QPGTTVFNCS HDFLSGKAAC 300
ISSGVHGISP IAEDVSLLAA ALRIQNMVSS GRRWLREQQH RRWRLHCLKL 350
QPLSPVPSDI EISRAQTPKA VEILAKEIGL LADEIEIYGK SKAKVRLSLL 400
ERLKDQADGK YVLVAGITPT PLGEGKSTVT IGLVQALTAH LNVNSFACLR 450
QPSQGPTFGV KGGAAGGGYA QVIPMEEFNL HLTGDIHAIT AANNLLAAAI 500
DARILHENTQ TDKALYNRLV PSVNGVREFS KIQLARLKRL GINKTDPSAL 550
TEEEMRKFAR LDIDPSTITW QRVVDTNDRF LRKITIGQAN TEKGCSRQAQ 600
FDIAVASEIM AVLALTDSLS DMKERLGRMV VASDRNGQPV TADDLGVTGA 650
LTVLMKDAIK PNLMQTLEGT PVFVHAGPFA NIAHGNSSVL ADKIALKLVG 700
EGGFVVTEAG FGADIGMEKF FNIKCRASGL VPSVVVLVAT VRALKMHGGG 750
PSVTAGVPLR KEYTEENLQL VADGCCNLEK QIQIAQLFGV PVVVALNVFK 800
TDTRAEIDLV CELAKRAGAF NAVPCYHWSI GGKGSVDLAW AVREAASKES 850
RFQFLYDVQL PIVEKIRTIA QSVYGAKDIE LSPEAQSKID RYTEQGFGNL 900
PICMAKTHLS LSHQPDKKGV PKGFILPISD VRASIGAGFI YPLVGTMSTM 950
PGLPTRPCFY DIDLDTETEQ VKGLF 975
Length:975
Mass (Da):105,227
Last modified:July 1, 2008 - v2
Checksum:i4B7D0B35164A580B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951E → G in AAI20039. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120038 mRNA. Translation: AAI20039.1.
BC148878 mRNA. Translation: AAI48879.1.
RefSeqiNP_001069486.1. NM_001076018.2.
UniGeneiBt.7490.

Genome annotation databases

GeneIDi534296.
KEGGibta:534296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120038 mRNA. Translation: AAI20039.1 .
BC148878 mRNA. Translation: AAI48879.1 .
RefSeqi NP_001069486.1. NM_001076018.2.
UniGenei Bt.7490.

3D structure databases

ProteinModelPortali Q0VCR7.
SMRi Q0VCR7. Positions 509-571.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q0VCR7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 534296.
KEGGi bta:534296.

Organism-specific databases

CTDi 25902.

Phylogenomic databases

eggNOGi COG0190.
HOGENOMi HOG000040280.
HOVERGENi HBG004916.
InParanoidi Q0VCR7.
KOi K13402.

Enzyme and pathway databases

UniPathwayi UPA00193 .

Miscellaneous databases

NextBioi 20876344.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
3.40.50.720. 2 hits.
HAMAPi MF_01543. FTHFS.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR016040. NAD(P)-bd_dom.
IPR027417. P-loop_NTPase.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view ]
PRINTSi PR00085. THFDHDRGNASE.
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver and Thymus.

Entry informationi

Entry nameiC1TM_BOVIN
AccessioniPrimary (citable) accession number: Q0VCR7
Secondary accession number(s): A6QNK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: April 16, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi