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Protein

NmrA-like family domain-containing protein 1

Gene

NMRAL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP+. Binding to NADPH is necessary to form a stable dimer (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92NADP; shared with dimeric partnerBy similarity1
Binding sitei133NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16NADPBy similarity6
Nucleotide bindingi37 – 41NADPBy similarity5
Nucleotide bindingi58 – 59NADPBy similarity2
Nucleotide bindingi79 – 81NADPBy similarity3
Nucleotide bindingi155 – 158NADPBy similarity4

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-BTA-70635. Urea cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
NmrA-like family domain-containing protein 1
Gene namesi
Name:NMRAL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

  • Cytoplasm
  • Cytoplasmperinuclear region
  • Nucleus

  • Note: Under normal redox growth conditions localizes in the cytoplasm and perinuclear region. Nuclear localization is promoted by increased intracellular nitric oxide and reduced NADPH/NADP+ ratios (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782031 – 299NmrA-like family domain-containing protein 1Add BLAST299

Proteomic databases

PaxDbiQ0VCN1.
PeptideAtlasiQ0VCN1.
PRIDEiQ0VCN1.

Expressioni

Gene expression databases

BgeeiENSBTAG00000010015.
ExpressionAtlasiQ0VCN1. differential.

Interactioni

Subunit structurei

Homodimer. Interacts with ASS1. Interaction is enhanced by low NADPH/NADP+ ratios, which results in inhibition of ASS1 activity (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013208.

Structurei

3D structure databases

ProteinModelPortaliQ0VCN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 189Interaction with ASS1By similarityAdd BLAST37

Sequence similaritiesi

Belongs to the NmrA-type oxidoreductase family.Curated

Phylogenomic databases

eggNOGiENOG410IVQ5. Eukaryota.
COG0702. LUCA.
GeneTreeiENSGT00390000016395.
HOGENOMiHOG000035269.
HOVERGENiHBG082032.
InParanoidiQ0VCN1.
OMAiPATFMEL.
OrthoDBiEOG091G0E3T.
TreeFamiTF335532.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0VCN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKKLVVVF GATGAQGGSV ARTLLEDGTF RVRVVTRDPG QRAAKQLRLQ
60 70 80 90 100
GAEVVQGDQD DEASMELALS GAHATFIVTN YWENCSQEQE VKQGKLLADL
110 120 130 140 150
AKRLGLRYVV YSGLENIKKL TAGRLTVGHF DGKGEVEEYF RDIGVPMTSV
160 170 180 190 200
RLPCYFENLL SYFLPQKAPD GRSYLLSLPM GDVPIDGMSV ADLGPVVLSL
210 220 230 240 250
LKTPEEYVGR NIGLSTCRHT VEEYAALLTK HTGKAVRDAK TSPEDYEKLG
260 270 280 290
FPGAQDLANM FRFYALKPDR NIELTLKLNP KARRLDQWLE QHKEDFAGL
Length:299
Mass (Da):33,154
Last modified:September 5, 2006 - v1
Checksum:i6A0CDCFB3A5CE904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120089 mRNA. Translation: AAI20090.1.
RefSeqiNP_001069500.1. NM_001076032.2.
XP_005224578.3. XM_005224521.3.
UniGeneiBt.53592.

Genome annotation databases

EnsembliENSBTAT00000013208; ENSBTAP00000013208; ENSBTAG00000010015.
GeneIDi534628.
KEGGibta:534628.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120089 mRNA. Translation: AAI20090.1.
RefSeqiNP_001069500.1. NM_001076032.2.
XP_005224578.3. XM_005224521.3.
UniGeneiBt.53592.

3D structure databases

ProteinModelPortaliQ0VCN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013208.

Proteomic databases

PaxDbiQ0VCN1.
PeptideAtlasiQ0VCN1.
PRIDEiQ0VCN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013208; ENSBTAP00000013208; ENSBTAG00000010015.
GeneIDi534628.
KEGGibta:534628.

Organism-specific databases

CTDi57407.

Phylogenomic databases

eggNOGiENOG410IVQ5. Eukaryota.
COG0702. LUCA.
GeneTreeiENSGT00390000016395.
HOGENOMiHOG000035269.
HOVERGENiHBG082032.
InParanoidiQ0VCN1.
OMAiPATFMEL.
OrthoDBiEOG091G0E3T.
TreeFamiTF335532.

Enzyme and pathway databases

ReactomeiR-BTA-70635. Urea cycle.

Gene expression databases

BgeeiENSBTAG00000010015.
ExpressionAtlasiQ0VCN1. differential.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNMRL1_BOVIN
AccessioniPrimary (citable) accession number: Q0VCN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: September 5, 2006
Last modified: November 30, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Lacks the conserved Tyr residue in the active site triad of Ser-Tyr-Lys necessary for dehydrogenase activity, suggesting that it has no oxidoreductase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.