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Protein

NmrA-like family domain-containing protein 1

Gene

NMRAL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP+ levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP+. Binding to NADPH is necessary to form a stable dimer (By similarity).By similarity

Caution

Lacks the conserved Tyr residue in the active site triad of Ser-Tyr-Lys necessary for dehydrogenase activity, suggesting that it has no oxidoreductase activity.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92NADP; shared with dimeric partnerBy similarity1
Binding sitei133NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16NADPBy similarity6
Nucleotide bindingi37 – 41NADPBy similarity5
Nucleotide bindingi58 – 59NADPBy similarity2
Nucleotide bindingi79 – 81NADPBy similarity3
Nucleotide bindingi155 – 158NADPBy similarity4

GO - Molecular functioni

Keywordsi

LigandNADP

Enzyme and pathway databases

ReactomeiR-BTA-70635 Urea cycle

Names & Taxonomyi

Protein namesi
Recommended name:
NmrA-like family domain-containing protein 1
Gene namesi
Name:NMRAL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Organism-specific databases

VGNCiVGNC:32134 NMRAL1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782031 – 299NmrA-like family domain-containing protein 1Add BLAST299

Proteomic databases

PaxDbiQ0VCN1
PeptideAtlasiQ0VCN1
PRIDEiQ0VCN1

Expressioni

Gene expression databases

BgeeiENSBTAG00000010015
ExpressionAtlasiQ0VCN1 baseline and differential

Interactioni

Subunit structurei

Homodimer. Interacts with ASS1. Interaction is enhanced by low NADPH/NADP+ ratios, which results in inhibition of ASS1 activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013208

Structurei

3D structure databases

ProteinModelPortaliQ0VCN1
SMRiQ0VCN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 189Interaction with ASS1By similarityAdd BLAST37

Sequence similaritiesi

Belongs to the NmrA-type oxidoreductase family.Curated

Phylogenomic databases

eggNOGiENOG410IVQ5 Eukaryota
COG0702 LUCA
GeneTreeiENSGT00390000016395
HOGENOMiHOG000035269
HOVERGENiHBG082032
InParanoidiQ0VCN1
OMAiQDDEASM
OrthoDBiEOG091G0E3T
TreeFamiTF335532

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR008030 NmrA-like
PfamiView protein in Pfam
PF05368 NmrA, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

Q0VCN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKKLVVVF GATGAQGGSV ARTLLEDGTF RVRVVTRDPG QRAAKQLRLQ
60 70 80 90 100
GAEVVQGDQD DEASMELALS GAHATFIVTN YWENCSQEQE VKQGKLLADL
110 120 130 140 150
AKRLGLRYVV YSGLENIKKL TAGRLTVGHF DGKGEVEEYF RDIGVPMTSV
160 170 180 190 200
RLPCYFENLL SYFLPQKAPD GRSYLLSLPM GDVPIDGMSV ADLGPVVLSL
210 220 230 240 250
LKTPEEYVGR NIGLSTCRHT VEEYAALLTK HTGKAVRDAK TSPEDYEKLG
260 270 280 290
FPGAQDLANM FRFYALKPDR NIELTLKLNP KARRLDQWLE QHKEDFAGL
Length:299
Mass (Da):33,154
Last modified:September 5, 2006 - v1
Checksum:i6A0CDCFB3A5CE904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120089 mRNA Translation: AAI20090.1
RefSeqiNP_001069500.1, NM_001076032.2
XP_005224578.3, XM_005224521.3
UniGeneiBt.53592

Genome annotation databases

EnsembliENSBTAT00000013208; ENSBTAP00000013208; ENSBTAG00000010015
GeneIDi534628
KEGGibta:534628

Similar proteinsi

Entry informationi

Entry nameiNMRL1_BOVIN
AccessioniPrimary (citable) accession number: Q0VCN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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