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Protein

Bifunctional purine biosynthesis protein PURH

Gene

ATIC

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.By similarity
Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization.By similarity

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PURH (ATIC)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PURH (ATIC)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei137Proton acceptorSequence analysis1
Sitei266Transition state stabilizerSequence analysis1
Active sitei267Proton acceptorCurated1
Binding sitei316AICAR; via carbonyl oxygenBy similarity1
Binding sitei339AICARBy similarity1
Binding sitei431AICAR; shared with dimeric partnerBy similarity1
Binding sitei451AICAR; shared with dimeric partnerBy similarity1
Binding sitei541AICAR; via carbonyl oxygen; shared with dimeric partnerBy similarity1
Binding sitei588AICAR; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 14IMPBy similarity3
Nucleotide bindingi34 – 37IMPBy similarity4
Nucleotide bindingi64 – 67IMPBy similarity4
Nucleotide bindingi101 – 104IMPBy similarity4
Nucleotide bindingi125 – 127IMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

ReactomeiR-BTA-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00133
UPA00074; UER00135

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PURH
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
Alternative name(s):
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
AICAR transformylase
IMP cyclohydrolase (EC:3.5.4.10)
Alternative name(s):
ATIC
IMP synthase
Inosinicase
Gene namesi
Name:ATIC
Synonyms:PURH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi

Organism-specific databases

VGNCiVGNC:26264 ATIC

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002702121 – 592Bifunctional purine biosynthesis protein PURHAdd BLAST592

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei199N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ0VCK0
PeptideAtlasiQ0VCK0
PRIDEiQ0VCK0

Expressioni

Gene expression databases

BgeeiENSBTAG00000019274

Interactioni

Subunit structurei

Homodimer (By similarity). Associates with internalized INSR complexes on Golgi/endosomal membranes. Interacts with INSR; ATIC together with PRKAA2/AMPK2 and HACD3/PTPLAD1 is proposed to be part of a signaling network regulating INSR autophosphorylation and endocytosis (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025662

Structurei

3D structure databases

ProteinModelPortaliQ0VCK0
SMRiQ0VCK0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 146MGS-likePROSITE-ProRule annotationAdd BLAST146

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 208AICAR bindingBy similarity2

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.

Sequence similaritiesi

Belongs to the PurH family.Curated

Phylogenomic databases

eggNOGiKOG2555 Eukaryota
COG0138 LUCA
GeneTreeiENSGT00390000004553
HOGENOMiHOG000230372
HOVERGENiHBG006912
InParanoidiQ0VCK0
KOiK00602
OMAiDLLFAWK
OrthoDBiEOG091G03H4
TreeFamiTF105642

Family and domain databases

Gene3Di1.10.287.440, 1 hit
3.40.140.20, 3 hits
3.40.50.1380, 1 hit
HAMAPiMF_00139 PurH, 1 hit
InterProiView protein in InterPro
IPR024051 AICAR_Tfase_dup_dom_sf
IPR024050 AICAR_Tfase_insert_dom_sf
IPR016193 Cytidine_deaminase-like
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR002695 PurH-like
PANTHERiPTHR11692 PTHR11692, 1 hit
PfamiView protein in Pfam
PF01808 AICARFT_IMPCHas, 1 hit
PF02142 MGS, 1 hit
PIRSFiPIRSF000414 AICARFT_IMPCHas, 1 hit
SMARTiView protein in SMART
SM00798 AICARFT_IMPCHas, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF52335 SSF52335, 1 hit
SSF53927 SSF53927, 1 hit
TIGRFAMsiTIGR00355 purH, 1 hit
PROSITEiView protein in PROSITE
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

Q0VCK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGQLALFS VSDKNGLVEF ARNLASVGLN LIASGGTAKA LRDAGLAVRD
60 70 80 90 100
VSELTGFPEM LGGRVKTLHP AVHAGILARD IPEDSADMAK LDFNLIRVVV
110 120 130 140 150
CNLYPFGKTV ASPGVTVEEA VEHIDIGGVT LLRAAAKNHA RVTVVCEPED
160 170 180 190 200
YAAVASEMQD SDSKDTSLET RRQLALKAFT HTAQYDEAIS DYFRKEYSKG
210 220 230 240 250
VSQMPLRYGM NPHQTPAQLY TLKPKLPITV LNGAPGFINL CDALNAWQLV
260 270 280 290 300
KELKEALGLP AAASFKHVSP AGAAVGIPLS EDEANVCMVY DLYKTLTPVA
310 320 330 340 350
TAYARARGAD RMSSFGDFVA LSDVCDVPTA KIISREVSDG IIAPGYENEA
360 370 380 390 400
LKILSKKKNG NYCVLQMDQS YIPDENEVRT LFGLRLSQKR NNSVVNRSLF
410 420 430 440 450
SNIVTKNKDL PESALRDLIV ATIAVKYTQS NSVCYAKNGQ VIGIGAGQQS
460 470 480 490 500
RIHCTRLAGD KANCWWLRHH PQVLSMKFKT GVKRAEISNA IDQYVTGTIG
510 520 530 540 550
EGEDLIKWKA LFEEVPELLT ETEKKEWIDK LNEVSISSDA FFPFRDNVDR
560 570 580 590
AKRSGVAYIA APSGSAADKV VIEACDELGI ILAHTNLRLF HH
Length:592
Mass (Da):64,483
Last modified:September 5, 2006 - v1
Checksum:iEBD94993555935D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120131 mRNA Translation: AAI20132.1
RefSeqiNP_001068722.1, NM_001075254.2
UniGeneiBt.61076

Genome annotation databases

EnsembliENSBTAT00000025662; ENSBTAP00000025662; ENSBTAG00000019274
GeneIDi506343
KEGGibta:506343

Similar proteinsi

Entry informationi

Entry nameiPUR9_BOVIN
AccessioniPrimary (citable) accession number: Q0VCK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: September 5, 2006
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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