Reviewed, UniProtKB/Swiss-Prot Q0VCK0 (PUR9_BOVIN)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein PURH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	ATIC
Synonyms:	PURH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region.
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Bifunctional purine biosynthesis protein PURH		PRO_0000270212
Amino acid modifications
	Modified residue	104	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VCK0-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: EBD94993555935D7	FASTA	592	64,483
	10 20 30 40 50 60 MAPGQLALFS VSDKNGLVEF ARNLASVGLN LIASGGTAKA LRDAGLAVRD VSELTGFPEM 70 80 90 100 110 120 LGGRVKTLHP AVHAGILARD IPEDSADMAK LDFNLIRVVV CNLYPFGKTV ASPGVTVEEA 130 140 150 160 170 180 VEHIDIGGVT LLRAAAKNHA RVTVVCEPED YAAVASEMQD SDSKDTSLET RRQLALKAFT 190 200 210 220 230 240 HTAQYDEAIS DYFRKEYSKG VSQMPLRYGM NPHQTPAQLY TLKPKLPITV LNGAPGFINL 250 260 270 280 290 300 CDALNAWQLV KELKEALGLP AAASFKHVSP AGAAVGIPLS EDEANVCMVY DLYKTLTPVA 310 320 330 340 350 360 TAYARARGAD RMSSFGDFVA LSDVCDVPTA KIISREVSDG IIAPGYENEA LKILSKKKNG 370 380 390 400 410 420 NYCVLQMDQS YIPDENEVRT LFGLRLSQKR NNSVVNRSLF SNIVTKNKDL PESALRDLIV 430 440 450 460 470 480 ATIAVKYTQS NSVCYAKNGQ VIGIGAGQQS RIHCTRLAGD KANCWWLRHH PQVLSMKFKT 490 500 510 520 530 540 GVKRAEISNA IDQYVTGTIG EGEDLIKWKA LFEEVPELLT ETEKKEWIDK LNEVSISSDA 550 560 570 580 590 FFPFRDNVDR AKRSGVAYIA APSGSAADKV VIEACDELGI ILAHTNLRLF HH

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal lung.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC120131 mRNA. Translation: AAI20132.1.
IPI	IPI00724051.
RefSeq	NP_001068722.1.
UniGene	Bt.61076
3D structure databases
SMR	Q0VCK0. Positions 5-592.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000019274. Bos taurus. [Contig view]
GeneID	506343.
KEGG	bta:506343.
Phylogenomic databases
HOVERGEN	Q0VCK0.
Enzyme and pathway databases
BRENDA	2.1.2.3. 251. 3.5.4.10. 251.
Family and domain databases
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHas_formly. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BOVIN

Accession

Primary (citable) accession number: Q0VCK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)