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Q0VCK0

- PUR9_BOVIN

UniProt

Q0VCK0 - PUR9_BOVIN

Protein

Bifunctional purine biosynthesis protein PURH

Gene

ATIC

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.By similarity

    Catalytic activityi

    10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
    IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei137 – 1371Proton acceptorSequence Analysis
    Sitei266 – 2661Transition state stabilizerSequence Analysis
    Active sitei267 – 2671Proton acceptorCurated
    Binding sitei316 – 3161AICAR; via carbonyl oxygenBy similarity
    Binding sitei339 – 3391AICARBy similarity
    Binding sitei431 – 4311AICAR; shared with dimeric partnerBy similarity
    Binding sitei451 – 4511AICAR; shared with dimeric partnerBy similarity
    Binding sitei541 – 5411AICAR; via carbonyl oxygen; shared with dimeric partnerBy similarity
    Binding sitei588 – 5881AICAR; shared with dimeric partnerBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 143IMPBy similarity
    Nucleotide bindingi34 – 374IMPBy similarity
    Nucleotide bindingi64 – 674IMPBy similarity
    Nucleotide bindingi101 – 1044IMPBy similarity
    Nucleotide bindingi125 – 1273IMPBy similarity

    GO - Molecular functioni

    1. IMP cyclohydrolase activity Source: UniProtKB-EC
    2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: UniProtKB-EC
    3. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_226478. Purine ribonucleoside monophosphate biosynthesis.
    UniPathwayiUPA00074; UER00133.
    UPA00074; UER00135.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional purine biosynthesis protein PURH
    Including the following 2 domains:
    Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
    Alternative name(s):
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
    AICAR transformylase
    IMP cyclohydrolase (EC:3.5.4.10)
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
    Gene namesi
    Name:ATIC
    Synonyms:PURH
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 2

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 592592Bifunctional purine biosynthesis protein PURHPRO_0000270212Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei199 – 1991N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ0VCK0.
    PRIDEiQ0VCK0.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000048873.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VCK0.
    SMRiQ0VCK0. Positions 4-592.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni207 – 2082AICAR bindingBy similarity

    Domaini

    The IMP cyclohydrolase activity resides in the N-terminal region.

    Sequence similaritiesi

    Belongs to the PurH family.Curated

    Phylogenomic databases

    eggNOGiCOG0138.
    GeneTreeiENSGT00390000004553.
    HOGENOMiHOG000230372.
    HOVERGENiHBG006912.
    InParanoidiQ0VCK0.
    KOiK00602.
    OMAiAGDKANC.
    OrthoDBiEOG74N5GD.
    TreeFamiTF105642.

    Family and domain databases

    Gene3Di1.10.287.440. 1 hit.
    3.40.140.20. 3 hits.
    3.40.50.1380. 1 hit.
    HAMAPiMF_00139. PurH.
    InterProiIPR024051. AICAR_Tfase_dom.
    IPR024050. AICAR_Tfase_insert_dom.
    IPR002695. AICARFT_IMPCHas.
    IPR016193. Cytidine_deaminase-like.
    IPR011607. MGS-like_dom.
    [Graphical view]
    PANTHERiPTHR11692. PTHR11692. 1 hit.
    PfamiPF01808. AICARFT_IMPCHas. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
    SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52335. SSF52335. 1 hit.
    SSF53927. SSF53927. 1 hit.
    TIGRFAMsiTIGR00355. purH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0VCK0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPGQLALFS VSDKNGLVEF ARNLASVGLN LIASGGTAKA LRDAGLAVRD    50
    VSELTGFPEM LGGRVKTLHP AVHAGILARD IPEDSADMAK LDFNLIRVVV 100
    CNLYPFGKTV ASPGVTVEEA VEHIDIGGVT LLRAAAKNHA RVTVVCEPED 150
    YAAVASEMQD SDSKDTSLET RRQLALKAFT HTAQYDEAIS DYFRKEYSKG 200
    VSQMPLRYGM NPHQTPAQLY TLKPKLPITV LNGAPGFINL CDALNAWQLV 250
    KELKEALGLP AAASFKHVSP AGAAVGIPLS EDEANVCMVY DLYKTLTPVA 300
    TAYARARGAD RMSSFGDFVA LSDVCDVPTA KIISREVSDG IIAPGYENEA 350
    LKILSKKKNG NYCVLQMDQS YIPDENEVRT LFGLRLSQKR NNSVVNRSLF 400
    SNIVTKNKDL PESALRDLIV ATIAVKYTQS NSVCYAKNGQ VIGIGAGQQS 450
    RIHCTRLAGD KANCWWLRHH PQVLSMKFKT GVKRAEISNA IDQYVTGTIG 500
    EGEDLIKWKA LFEEVPELLT ETEKKEWIDK LNEVSISSDA FFPFRDNVDR 550
    AKRSGVAYIA APSGSAADKV VIEACDELGI ILAHTNLRLF HH 592
    Length:592
    Mass (Da):64,483
    Last modified:September 5, 2006 - v1
    Checksum:iEBD94993555935D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120131 mRNA. Translation: AAI20132.1.
    RefSeqiNP_001068722.1. NM_001075254.2.
    UniGeneiBt.61076.

    Genome annotation databases

    EnsembliENSBTAT00000025662; ENSBTAP00000025662; ENSBTAG00000019274.
    GeneIDi506343.
    KEGGibta:506343.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120131 mRNA. Translation: AAI20132.1 .
    RefSeqi NP_001068722.1. NM_001075254.2.
    UniGenei Bt.61076.

    3D structure databases

    ProteinModelPortali Q0VCK0.
    SMRi Q0VCK0. Positions 4-592.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000048873.

    Proteomic databases

    PaxDbi Q0VCK0.
    PRIDEi Q0VCK0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000025662 ; ENSBTAP00000025662 ; ENSBTAG00000019274 .
    GeneIDi 506343.
    KEGGi bta:506343.

    Organism-specific databases

    CTDi 471.

    Phylogenomic databases

    eggNOGi COG0138.
    GeneTreei ENSGT00390000004553.
    HOGENOMi HOG000230372.
    HOVERGENi HBG006912.
    InParanoidi Q0VCK0.
    KOi K00602.
    OMAi AGDKANC.
    OrthoDBi EOG74N5GD.
    TreeFami TF105642.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00133 .
    UPA00074 ; UER00135 .
    Reactomei REACT_226478. Purine ribonucleoside monophosphate biosynthesis.

    Miscellaneous databases

    NextBioi 20867570.

    Family and domain databases

    Gene3Di 1.10.287.440. 1 hit.
    3.40.140.20. 3 hits.
    3.40.50.1380. 1 hit.
    HAMAPi MF_00139. PurH.
    InterProi IPR024051. AICAR_Tfase_dom.
    IPR024050. AICAR_Tfase_insert_dom.
    IPR002695. AICARFT_IMPCHas.
    IPR016193. Cytidine_deaminase-like.
    IPR011607. MGS-like_dom.
    [Graphical view ]
    PANTHERi PTHR11692. PTHR11692. 1 hit.
    Pfami PF01808. AICARFT_IMPCHas. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000414. AICARFT_IMPCHas. 1 hit.
    SMARTi SM00798. AICARFT_IMPCHas. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52335. SSF52335. 1 hit.
    SSF53927. SSF53927. 1 hit.
    TIGRFAMsi TIGR00355. purH. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal lung.

    Entry informationi

    Entry nameiPUR9_BOVIN
    AccessioniPrimary (citable) accession number: Q0VCK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3