Q0VCB2 (HDAC8_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone deacetylase 8
Short name=HD8
EC=3.5.1.98

Gene names

Name:

HDAC8

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility By similarity.
Catalytic activity	Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.
Cofactor	Binds 1 divalent metal cation per subunit.
Enzyme regulation	Its activity is inhibited by trichostatin A (TSA) and butyrate, 2 well known histone deacetylase inhibitors By similarity.
Subunit structure	Interacts with CBFA2T3 By similarity. Interacts with phosphorylated SMG5/EST1B; this interaction protects SMG5 from ubiquitin-mediated degradation. Associates with alpha-SMA (smooth muscle alpha-actin).
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Post-translational modification	Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4 By similarity.
Sequence similarities	Belongs to the histone deacetylase family. HD type 1 subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding
Molecular function	Chromatin regulator Hydrolase Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H3 deacetylation Inferred from electronic annotation. Source: GOC histone H4 deacetylation Inferred from electronic annotation. Source: GOC regulation of cohesin localization to chromatin Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW sister chromatid cohesion Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NAD-dependent histone deacetylase activity (H3-K14 specific) Inferred from electronic annotation. Source: EC NAD-dependent histone deacetylase activity (H3-K18 specific) Inferred from electronic annotation. Source: EC NAD-dependent histone deacetylase activity (H3-K9 specific) Inferred from electronic annotation. Source: EC NAD-dependent histone deacetylase activity (H4-K16 specific) Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Histone deacetylase 8		PRO_0000389507
Regions
	Region	14 – 324	311	Histone deacetylase By similarity
Sites
	Active site	143	1	Proton acceptor By similarity
	Metal binding	178	1	Divalent metal cation By similarity
	Metal binding	180	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	151	1	Substrate; via carbonyl oxygen By similarity
	Binding site	306	1	Substrate By similarity
Amino acid modifications
	Modified residue	39	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VCB2 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 1120A71660F14E7D	FASTA	377	41,683
	10 20 30 40 50 60 MEESEEPADA GQSLPPVYIY SPEYVSVCDS LAKVPKRASM VHSLIEAYAL HKQMRIVKPK 70 80 90 100 110 120 VASMEEMASF HTDAYLQHLQ KVSEDGDDDH PDSIEYGLGY DCPATEGIFD YAAAVGGATI 130 140 150 160 170 180 TAAQCLIDGM CKVAINWSGG WHHAKKDEAS GFCYLNDAVL GILRLRRKFD RILYVDLDLH 190 200 210 220 230 240 HGDGVEDAFS FTSKVMTVSL HKFSPGFFPG TGDVSDVGLG KGRYYSVNVP IQDCIQDERY 250 260 270 280 290 300 YHICESVLKE VYIAFNPKAV VLQLGADTIA GDPMCSFNMT PVGIGKCLKY ILQWELATLI 310 320 330 340 350 360 LGGGGYNLAN TARCWTYLTG VILGKTLSSE IPDHEFFTAY GPDYVLEITP SCRPDRNEPH 370 RVQQILNYIK GNLKHVV

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC120257 mRNA. Translation: AAI20258.1.
IPI	IPI00707449.
RefSeq	NP_001069699.1. NM_001076231.2.
UniGene	Bt.90254.
3D structure databases
ProteinModelPortal	Q0VCB2.
SMR	Q0VCB2. Positions 14-377.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	540666.
KEGG	bta:540666.
Organism-specific databases
CTD	55869.
Phylogenomic databases
HOVERGEN	HBG057112.
KO	K11405.
Family and domain databases
Gene3D	3.40.800.20. 1 hit.
InterPro	IPR000286. His_deacetylse. IPR003084. His_deacetylse_1. IPR023801. His_deacetylse_dom. [Graphical view]
PANTHER	PTHR10625. PTHR10625. 1 hit.
Pfam	PF00850. Hist_deacetyl. 1 hit. [Graphical view]
PIRSF	PIRSF037913. His_deacetylse_1. 1 hit.
PRINTS	PR01270. HDASUPER. PR01271. HISDACETLASE.
ProtoNet	Search...
Other
NextBio	20878759.

Entry information

Entry name

HDAC8_BOVIN

Accession

Primary (citable) accession number: Q0VCB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	September 5, 2006
Last modified:	April 3, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program