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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

HAAO

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Pathway:iNAD(+) biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (KMO)
  2. Kynureninase (KYNU)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (HAAO)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431DioxygenUniRule annotation
Metal bindingi47 – 471Iron; catalytic
Metal bindingi53 – 531Iron; catalytic
Binding sitei53 – 531SubstrateUniRule annotation
Metal bindingi91 – 911Iron; catalytic
Binding sitei95 – 951SubstrateUniRule annotation
Binding sitei105 – 1051SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.6. 908.
ReactomeiREACT_298704. Tryptophan catabolism.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Gene namesi
Name:HAAOUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 11

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2862863-hydroxyanthranilate 3,4-dioxygenasePRO_0000283033Add
BLAST

Proteomic databases

PRIDEiQ0VCA8.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006132.

Structurei

Secondary structure

1
286
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Helixi7 – 148Combined sources
Helixi15 – 173Combined sources
Turni20 – 223Combined sources
Beta strandi24 – 4118Combined sources
Beta strandi46 – 483Combined sources
Beta strandi53 – 608Combined sources
Beta strandi62 – 687Combined sources
Beta strandi71 – 777Combined sources
Beta strandi81 – 855Combined sources
Beta strandi91 – 955Combined sources
Beta strandi100 – 1067Combined sources
Beta strandi114 – 1207Combined sources
Beta strandi126 – 1327Combined sources
Turni137 – 1393Combined sources
Helixi141 – 1499Combined sources
Helixi152 – 1565Combined sources
Helixi161 – 1633Combined sources
Helixi183 – 1886Combined sources
Helixi191 – 1966Combined sources
Beta strandi200 – 2034Combined sources
Beta strandi207 – 2148Combined sources
Beta strandi216 – 2238Combined sources
Beta strandi228 – 2358Combined sources
Beta strandi237 – 2437Combined sources
Beta strandi245 – 2484Combined sources
Beta strandi252 – 2565Combined sources
Beta strandi261 – 2666Combined sources
Beta strandi271 – 2777Combined sources
Helixi279 – 2813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FE5X-ray2.51A2-286[»]
ProteinModelPortaliQ0VCA8.
SMRiQ0VCA8. Positions 2-286.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0VCA8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 160160Domain A (catalytic)Add
BLAST
Regioni161 – 17717LinkerAdd
BLAST
Regioni178 – 286109Domain BAdd
BLAST

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

eggNOGiNOG77058.
GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
HOVERGENiHBG000018.
InParanoidiQ0VCA8.
KOiK00452.
OMAiPARIPHS.
OrthoDBiEOG7FFMS4.
TreeFamiTF300246.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
PIRSFiPIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMiSSF51182. SSF51182. 2 hits.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0VCA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPVRVKAW VEENRGSFLP PVCNKLLHQK QLKIMFVGGP NTRKDYHIEE
60 70 80 90 100
GEEVFYQLEG DMLLRVLERG KHRDVVIRQG EIFLLPAGVP HSPQRFANTV
110 120 130 140 150
GLVIERRRLK TELDGLRYYV GDTTDVLFEK WFYCEDLGTQ LAPIIQEFFS
160 170 180 190 200
SEQYRTGKPN PDQLLKEPPF PLSTRSVMEP MCLEAWLDGH RKELQAGTPL
210 220 230 240 250
SLFGDTYESQ VMVHGQGSSE GLRRDVDVWL WQLEGSSVVT MEGQRLSLTL
260 270 280
DDSLLVPAGT LYGWERGQGS VALSVTQDPA CKKSLG
Length:286
Mass (Da):32,493
Last modified:September 5, 2006 - v1
Checksum:i6887DDB93B861F8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120265 mRNA. Translation: AAI20266.1.
RefSeqiNP_001068926.1. NM_001075458.2.
UniGeneiBt.20241.

Genome annotation databases

EnsembliENSBTAT00000006132; ENSBTAP00000006132; ENSBTAG00000004674.
GeneIDi510602.
KEGGibta:510602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120265 mRNA. Translation: AAI20266.1.
RefSeqiNP_001068926.1. NM_001075458.2.
UniGeneiBt.20241.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FE5X-ray2.51A2-286[»]
ProteinModelPortaliQ0VCA8.
SMRiQ0VCA8. Positions 2-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006132.

Proteomic databases

PRIDEiQ0VCA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006132; ENSBTAP00000006132; ENSBTAG00000004674.
GeneIDi510602.
KEGGibta:510602.

Organism-specific databases

CTDi23498.

Phylogenomic databases

eggNOGiNOG77058.
GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
HOVERGENiHBG000018.
InParanoidiQ0VCA8.
KOiK00452.
OMAiPARIPHS.
OrthoDBiEOG7FFMS4.
TreeFamiTF300246.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.
BRENDAi1.13.11.6. 908.
ReactomeiREACT_298704. Tryptophan catabolism.

Miscellaneous databases

EvolutionaryTraceiQ0VCA8.
NextBioi20869529.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00825. 3_HAO.
InterProiIPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
PIRSFiPIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMiSSF51182. SSF51182. 2 hits.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal liver.
  2. "Crystal structure of bovine 3-hydroxyanthranilate 3,4-dioxygenase."
    Dilovic I., Gliubich F., Malpeli G., Zanotti G., Matkovic-Calogovic D.
    Biopolymers 91:1189-1195(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.51 ANGSTROMS) OF 2-286, SUBUNIT, IRON-BINDING SITES.

Entry informationi

Entry namei3HAO_BOVIN
AccessioniPrimary (citable) accession number: Q0VCA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: September 5, 2006
Last modified: July 22, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.