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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

HAAO

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (KMO)
  2. Kynureninase (KYNU)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (HAAO)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43DioxygenUniRule annotation1
Metal bindingi47Iron; catalytic1
Metal bindingi53Iron; catalytic1
Binding sitei53SubstrateUniRule annotation1
Metal bindingi91Iron; catalytic1
Binding sitei95SubstrateUniRule annotation1
Binding sitei105SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.6. 908.
ReactomeiR-BTA-71240. Tryptophan catabolism.
UniPathwayiUPA00253; UER00330.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Gene namesi
Name:HAAOUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002830331 – 2863-hydroxyanthranilate 3,4-dioxygenaseAdd BLAST286

Proteomic databases

PaxDbiQ0VCA8.
PeptideAtlasiQ0VCA8.
PRIDEiQ0VCA8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000004674.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006132.

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Helixi7 – 14Combined sources8
Helixi15 – 17Combined sources3
Turni20 – 22Combined sources3
Beta strandi24 – 41Combined sources18
Beta strandi46 – 48Combined sources3
Beta strandi53 – 60Combined sources8
Beta strandi62 – 68Combined sources7
Beta strandi71 – 77Combined sources7
Beta strandi81 – 85Combined sources5
Beta strandi91 – 95Combined sources5
Beta strandi100 – 106Combined sources7
Beta strandi114 – 120Combined sources7
Beta strandi126 – 132Combined sources7
Turni137 – 139Combined sources3
Helixi141 – 149Combined sources9
Helixi152 – 156Combined sources5
Helixi161 – 163Combined sources3
Helixi183 – 188Combined sources6
Helixi191 – 196Combined sources6
Beta strandi200 – 203Combined sources4
Beta strandi207 – 214Combined sources8
Beta strandi216 – 223Combined sources8
Beta strandi228 – 235Combined sources8
Beta strandi237 – 243Combined sources7
Beta strandi245 – 248Combined sources4
Beta strandi252 – 256Combined sources5
Beta strandi261 – 266Combined sources6
Beta strandi271 – 277Combined sources7
Helixi279 – 281Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FE5X-ray2.51A2-286[»]
ProteinModelPortaliQ0VCA8.
SMRiQ0VCA8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ0VCA8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 160Domain A (catalytic)Add BLAST160
Regioni161 – 177LinkerAdd BLAST17
Regioni178 – 286Domain BAdd BLAST109

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3995. Eukaryota.
ENOG4111GH8. LUCA.
GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
HOVERGENiHBG000018.
InParanoidiQ0VCA8.
KOiK00452.
OMAiCKDLGTQ.
OrthoDBiEOG091G0IJ1.
TreeFamiTF300246.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00825. 3_HAO. 1 hit.
InterProiIPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
PIRSFiPIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMiSSF51182. SSF51182. 2 hits.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0VCA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPVRVKAW VEENRGSFLP PVCNKLLHQK QLKIMFVGGP NTRKDYHIEE
60 70 80 90 100
GEEVFYQLEG DMLLRVLERG KHRDVVIRQG EIFLLPAGVP HSPQRFANTV
110 120 130 140 150
GLVIERRRLK TELDGLRYYV GDTTDVLFEK WFYCEDLGTQ LAPIIQEFFS
160 170 180 190 200
SEQYRTGKPN PDQLLKEPPF PLSTRSVMEP MCLEAWLDGH RKELQAGTPL
210 220 230 240 250
SLFGDTYESQ VMVHGQGSSE GLRRDVDVWL WQLEGSSVVT MEGQRLSLTL
260 270 280
DDSLLVPAGT LYGWERGQGS VALSVTQDPA CKKSLG
Length:286
Mass (Da):32,493
Last modified:September 5, 2006 - v1
Checksum:i6887DDB93B861F8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120265 mRNA. Translation: AAI20266.1.
RefSeqiNP_001068926.1. NM_001075458.2.
UniGeneiBt.20241.

Genome annotation databases

EnsembliENSBTAT00000006132; ENSBTAP00000006132; ENSBTAG00000004674.
GeneIDi510602.
KEGGibta:510602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120265 mRNA. Translation: AAI20266.1.
RefSeqiNP_001068926.1. NM_001075458.2.
UniGeneiBt.20241.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FE5X-ray2.51A2-286[»]
ProteinModelPortaliQ0VCA8.
SMRiQ0VCA8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006132.

Proteomic databases

PaxDbiQ0VCA8.
PeptideAtlasiQ0VCA8.
PRIDEiQ0VCA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006132; ENSBTAP00000006132; ENSBTAG00000004674.
GeneIDi510602.
KEGGibta:510602.

Organism-specific databases

CTDi23498.

Phylogenomic databases

eggNOGiKOG3995. Eukaryota.
ENOG4111GH8. LUCA.
GeneTreeiENSGT00390000013008.
HOGENOMiHOG000218448.
HOVERGENiHBG000018.
InParanoidiQ0VCA8.
KOiK00452.
OMAiCKDLGTQ.
OrthoDBiEOG091G0IJ1.
TreeFamiTF300246.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00330.
BRENDAi1.13.11.6. 908.
ReactomeiR-BTA-71240. Tryptophan catabolism.

Miscellaneous databases

EvolutionaryTraceiQ0VCA8.

Gene expression databases

BgeeiENSBTAG00000004674.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00825. 3_HAO. 1 hit.
InterProiIPR010329. 3hydroanth_dOase.
IPR016700. 3hydroanth_dOase_met.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR15497. PTHR15497. 1 hit.
PfamiPF06052. 3-HAO. 1 hit.
[Graphical view]
PIRSFiPIRSF017681. 3hydroanth_dOase_animal. 1 hit.
SUPFAMiSSF51182. SSF51182. 2 hits.
TIGRFAMsiTIGR03037. anthran_nbaC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei3HAO_BOVIN
AccessioniPrimary (citable) accession number: Q0VCA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: September 5, 2006
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.