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Q0VCA5 (SAMH1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Short name=dNTPase
EC=3.1.5.-
Gene names
Name:SAMHD1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Host restriction nuclease that blocks early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. May function by reducing the cellular dNTP levels to levels too low for retroviral reverse transcription to occur. May play a role in mediating proinflammatory responses to TNF-alpha signaling By similarity.

Catalytic activity

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP By similarity.

Subunit structure

Homodimer. Homotetramer; in dGTP-bound form By similarity.

Subcellular location

Nucleus By similarity.

Post-translational modification

Ubiquitinated and targeted for proteasomal degradation by a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase with the help of the viral accessory protein Vpx By similarity.

Sequence similarities

Belongs to the SAMHD1 family.

Contains 1 HD domain.

Contains 1 SAM (sterile alpha motif) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 589589Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
PRO_0000361968

Regions

Domain45 – 10056SAM
Domain152 – 308157HD
Nucleotide binding125 – 1339dGTP By similarity
Nucleotide binding125 – 1339dGTP 1 By similarity
Nucleotide binding341 – 3433dGTP 2 By similarity
Region298 – 3047Substrate binding By similarity
Region359 – 3646Substrate binding By similarity

Sites

Active site2211 By similarity
Metal binding1551Zinc; via tele nitrogen By similarity
Metal binding1941Zinc; via tele nitrogen By similarity
Metal binding1951Zinc By similarity
Metal binding3001Zinc By similarity
Binding site1041dGTP 1 By similarity
Binding site1071dGTP 2; via amide nitrogen; shared with neighboring subunit By similarity
Binding site1371Substrate By similarity
Binding site1521Substrate By similarity
Binding site1981Substrate By similarity
Binding site3041Substrate By similarity
Binding site3081Substrate By similarity
Binding site3221dGTP 2 By similarity
Binding site3471dGTP 2 By similarity
Binding site3551Substrate By similarity
Binding site3651dGTP 2; shared with neighboring subunit By similarity
Binding site3661dGTP 2; shared with neighboring subunit By similarity
Binding site4401dGTP 1; shared with neighboring subunit By similarity
Binding site4441dGTP 1; shared with neighboring subunit By similarity
Binding site5121dGTP 2 By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue181Phosphoserine By similarity
Modified residue211Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0VCA5 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 260B7E035B2A6F85

FASTA58968,239
        10         20         30         40         50         60 
MQSADSQNTP KRPRRDGSPR TPPDSPLADA ETSPSHDLDP DYRTWGPEQV WSFLRRCGFS 

        70         80         90        100        110        120 
DSELLKRCRE KRMSGSLLPF PEDLGISSHG KKMKLLNCIQ DTMKVINDPI HGHIEFHPLL 

       130        140        150        160        170        180 
MRIIDTPQFQ RLRYIKQLGG GYYVFPGASH NRFEHSLGVG YLAGRLVREL SEKQPELQIS 

       190        200        210        220        230        240 
ERDILCVQIA GLCHDLGHGP FSHMFDGRFI PLARPEIKWT HEQGSVMMFE HLINSNGLQD 

       250        260        270        280        290        300 
VMKYYGLIPE EDILFIKEQI TGPPESPIKD ASKWLYKGRP KEKSFLYEIV ANKRNGIDVD 

       310        320        330        340        350        360 
KWDYFARDCH HLGIQNSFDY KRFLKFARVC EVDNMKHICT REKEVGNLYD MFHTRNCLHR 

       370        380        390        400        410        420 
RAYQHKVGNI IDTMITDAFL KADDHIEITG SAGRKYHIST AIDDMEAFTK LTDNIFLEIL 

       430        440        450        460        470        480 
YSTDPNLNDA RMILKKIESR NLYKFVGETQ PMIQRIKKEN YEHLPNEVAS AKPSDVELEA 

       490        500        510        520        530        540 
ELKAEDLIVD VINMDYGMED KNPIDHVRFY CKSDLSKAVM ITRNQVSQFL PETFAEQLIR 

       550        560        570        580 
VYCKKTDEKT LFAARQHFVH WCLINDFTKP QIKKLPLRKL KKELTTATG 

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References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal medulla.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC120271 mRNA. Translation: AAI20272.1.
RefSeqNP_001069329.1. NM_001075861.1.
UniGeneBt.92388.

3D structure databases

ProteinModelPortalQ0VCA5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000041236.

Proteomic databases

PaxDbQ0VCA5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID524683.
KEGGbta:524683.

Organism-specific databases

CTD25939.

Phylogenomic databases

eggNOGCOG1078.
HOGENOMHOG000264286.
HOVERGENHBG054208.
InParanoidQ0VCA5.

Family and domain databases

Gene3D1.10.3210.10. 2 hits.
InterProIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013761. SAM/pointed.
[Graphical view]
PfamPF01966. HD. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
ProtoNetSearch...

Other

NextBio20874017.

Entry information

Entry nameSAMH1_BOVIN
AccessionPrimary (citable) accession number: Q0VCA5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families