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Protein

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Gene

SAMHD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Host restriction nuclease that blocks early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. May function by reducing the cellular dNTP levels to levels too low for retroviral reverse transcription to occur. May play a role in mediating proinflammatory responses to TNF-alpha signaling (By similarity).By similarity

Catalytic activityi

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104dGTP 1By similarity1
Binding sitei107dGTP 2; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei137SubstrateBy similarity1
Binding sitei152SubstrateBy similarity1
Metal bindingi155Zinc; via tele nitrogenBy similarity1
Metal bindingi194Zinc; via tele nitrogenBy similarity1
Metal bindingi195ZincBy similarity1
Binding sitei198SubstrateBy similarity1
Active sitei221By similarity1
Metal bindingi300ZincBy similarity1
Binding sitei304SubstrateBy similarity1
Binding sitei308SubstrateBy similarity1
Binding sitei322dGTP 2By similarity1
Binding sitei347dGTP 2By similarity1
Binding sitei355SubstrateBy similarity1
Binding sitei365dGTP 2; shared with neighboring subunitBy similarity1
Binding sitei366dGTP 2; shared with neighboring subunitBy similarity1
Binding sitei440dGTP 1; shared with neighboring subunitBy similarity1
Binding sitei444dGTP 1; shared with neighboring subunitBy similarity1
Binding sitei512dGTP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 133dGTPBy similarity9
Nucleotide bindingi125 – 133dGTP 1By similarity9
Nucleotide bindingi341 – 343dGTP 2By similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
Short name:
dNTPase
Gene namesi
Name:SAMHD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003619681 – 589Deoxynucleoside triphosphate triphosphohydrolase SAMHD1Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei18PhosphoserineBy similarity1
Modified residuei21PhosphothreonineBy similarity1
Modified residuei33PhosphoserineBy similarity1
Modified residuei88PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated and targeted for proteasomal degradation by a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase with the help of the viral accessory protein Vpx.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ0VCA5.
PeptideAtlasiQ0VCA5.
PRIDEiQ0VCA5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000022007.

Interactioni

Subunit structurei

Homodimer. Homotetramer; in dGTP-bound form (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041236.

Structurei

3D structure databases

ProteinModelPortaliQ0VCA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 100SAMAdd BLAST56
Domaini152 – 308HDAdd BLAST157

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 304Substrate bindingBy similarity7
Regioni359 – 364Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the SAMHD1 family.Curated
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiKOG2681. Eukaryota.
COG1078. LUCA.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ0VCA5.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0VCA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSADSQNTP KRPRRDGSPR TPPDSPLADA ETSPSHDLDP DYRTWGPEQV
60 70 80 90 100
WSFLRRCGFS DSELLKRCRE KRMSGSLLPF PEDLGISSHG KKMKLLNCIQ
110 120 130 140 150
DTMKVINDPI HGHIEFHPLL MRIIDTPQFQ RLRYIKQLGG GYYVFPGASH
160 170 180 190 200
NRFEHSLGVG YLAGRLVREL SEKQPELQIS ERDILCVQIA GLCHDLGHGP
210 220 230 240 250
FSHMFDGRFI PLARPEIKWT HEQGSVMMFE HLINSNGLQD VMKYYGLIPE
260 270 280 290 300
EDILFIKEQI TGPPESPIKD ASKWLYKGRP KEKSFLYEIV ANKRNGIDVD
310 320 330 340 350
KWDYFARDCH HLGIQNSFDY KRFLKFARVC EVDNMKHICT REKEVGNLYD
360 370 380 390 400
MFHTRNCLHR RAYQHKVGNI IDTMITDAFL KADDHIEITG SAGRKYHIST
410 420 430 440 450
AIDDMEAFTK LTDNIFLEIL YSTDPNLNDA RMILKKIESR NLYKFVGETQ
460 470 480 490 500
PMIQRIKKEN YEHLPNEVAS AKPSDVELEA ELKAEDLIVD VINMDYGMED
510 520 530 540 550
KNPIDHVRFY CKSDLSKAVM ITRNQVSQFL PETFAEQLIR VYCKKTDEKT
560 570 580
LFAARQHFVH WCLINDFTKP QIKKLPLRKL KKELTTATG
Length:589
Mass (Da):68,239
Last modified:September 5, 2006 - v1
Checksum:i260B7E035B2A6F85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120271 mRNA. Translation: AAI20272.1.
RefSeqiNP_001069329.1. NM_001075861.1.
UniGeneiBt.92388.

Genome annotation databases

GeneIDi524683.
KEGGibta:524683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC120271 mRNA. Translation: AAI20272.1.
RefSeqiNP_001069329.1. NM_001075861.1.
UniGeneiBt.92388.

3D structure databases

ProteinModelPortaliQ0VCA5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041236.

Proteomic databases

PaxDbiQ0VCA5.
PeptideAtlasiQ0VCA5.
PRIDEiQ0VCA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi524683.
KEGGibta:524683.

Organism-specific databases

CTDi25939.

Phylogenomic databases

eggNOGiKOG2681. Eukaryota.
COG1078. LUCA.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ0VCA5.

Gene expression databases

BgeeiENSBTAG00000022007.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSAMH1_BOVIN
AccessioniPrimary (citable) accession number: Q0VCA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 5, 2006
Last modified: October 5, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.