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Q0VCA5

- SAMH1_BOVIN

UniProt

Q0VCA5 - SAMH1_BOVIN

Protein

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Gene

SAMHD1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Host restriction nuclease that blocks early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. May function by reducing the cellular dNTP levels to levels too low for retroviral reverse transcription to occur. May play a role in mediating proinflammatory responses to TNF-alpha signaling By similarity.By similarity

    Catalytic activityi

    dNTP + H2O = Deoxynucleoside + triphosphate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Enzyme regulationi

    Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041dGTP 1By similarity
    Binding sitei107 – 1071dGTP 2; via amide nitrogen; shared with neighboring subunitBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Metal bindingi155 – 1551Zinc; via tele nitrogenBy similarity
    Metal bindingi194 – 1941Zinc; via tele nitrogenBy similarity
    Metal bindingi195 – 1951ZincBy similarity
    Binding sitei198 – 1981SubstrateBy similarity
    Active sitei221 – 2211By similarity
    Metal bindingi300 – 3001ZincBy similarity
    Binding sitei304 – 3041SubstrateBy similarity
    Binding sitei308 – 3081SubstrateBy similarity
    Binding sitei322 – 3221dGTP 2By similarity
    Binding sitei347 – 3471dGTP 2By similarity
    Binding sitei355 – 3551SubstrateBy similarity
    Binding sitei365 – 3651dGTP 2; shared with neighboring subunitBy similarity
    Binding sitei366 – 3661dGTP 2; shared with neighboring subunitBy similarity
    Binding sitei440 – 4401dGTP 1; shared with neighboring subunitBy similarity
    Binding sitei444 – 4441dGTP 1; shared with neighboring subunitBy similarity
    Binding sitei512 – 5121dGTP 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi125 – 1339dGTPBy similarity
    Nucleotide bindingi125 – 1339dGTP 1By similarity
    Nucleotide bindingi341 – 3433dGTP 2By similarity

    GO - Molecular functioni

    1. dGTPase activity Source: UniProtKB
    2. dGTP binding Source: UniProtKB
    3. nucleic acid binding Source: UniProtKB
    4. phosphoric diester hydrolase activity Source: InterPro
    5. RNA binding Source: UniProtKB
    6. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. dATP catabolic process Source: UniProtKB
    2. defense response to virus Source: UniProtKB
    3. dGTP catabolic process Source: UniProtKB
    4. innate immune response Source: UniProtKB-KW
    5. regulation of innate immune response Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Immunity, Innate immunity

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
    Short name:
    dNTPase
    Gene namesi
    Name:SAMHD1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 589589Deoxynucleoside triphosphate triphosphohydrolase SAMHD1PRO_0000361968Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei18 – 181PhosphoserineBy similarity
    Modified residuei21 – 211PhosphothreonineBy similarity

    Post-translational modificationi

    Ubiquitinated and targeted for proteasomal degradation by a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase with the help of the viral accessory protein Vpx.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ0VCA5.

    Interactioni

    Subunit structurei

    Homodimer. Homotetramer; in dGTP-bound form By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000041236.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VCA5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 10056SAMAdd
    BLAST
    Domaini152 – 308157HDAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni298 – 3047Substrate bindingBy similarity
    Regioni359 – 3646Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the SAMHD1 family.Curated
    Contains 1 HD domain.Curated

    Phylogenomic databases

    eggNOGiCOG1078.
    HOGENOMiHOG000264286.
    HOVERGENiHBG054208.
    InParanoidiQ0VCA5.

    Family and domain databases

    Gene3Di1.10.3210.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR013761. SAM/pointed.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q0VCA5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQSADSQNTP KRPRRDGSPR TPPDSPLADA ETSPSHDLDP DYRTWGPEQV    50
    WSFLRRCGFS DSELLKRCRE KRMSGSLLPF PEDLGISSHG KKMKLLNCIQ 100
    DTMKVINDPI HGHIEFHPLL MRIIDTPQFQ RLRYIKQLGG GYYVFPGASH 150
    NRFEHSLGVG YLAGRLVREL SEKQPELQIS ERDILCVQIA GLCHDLGHGP 200
    FSHMFDGRFI PLARPEIKWT HEQGSVMMFE HLINSNGLQD VMKYYGLIPE 250
    EDILFIKEQI TGPPESPIKD ASKWLYKGRP KEKSFLYEIV ANKRNGIDVD 300
    KWDYFARDCH HLGIQNSFDY KRFLKFARVC EVDNMKHICT REKEVGNLYD 350
    MFHTRNCLHR RAYQHKVGNI IDTMITDAFL KADDHIEITG SAGRKYHIST 400
    AIDDMEAFTK LTDNIFLEIL YSTDPNLNDA RMILKKIESR NLYKFVGETQ 450
    PMIQRIKKEN YEHLPNEVAS AKPSDVELEA ELKAEDLIVD VINMDYGMED 500
    KNPIDHVRFY CKSDLSKAVM ITRNQVSQFL PETFAEQLIR VYCKKTDEKT 550
    LFAARQHFVH WCLINDFTKP QIKKLPLRKL KKELTTATG 589
    Length:589
    Mass (Da):68,239
    Last modified:September 5, 2006 - v1
    Checksum:i260B7E035B2A6F85
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120271 mRNA. Translation: AAI20272.1.
    RefSeqiNP_001069329.1. NM_001075861.1.
    UniGeneiBt.92388.

    Genome annotation databases

    GeneIDi524683.
    KEGGibta:524683.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120271 mRNA. Translation: AAI20272.1 .
    RefSeqi NP_001069329.1. NM_001075861.1.
    UniGenei Bt.92388.

    3D structure databases

    ProteinModelPortali Q0VCA5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000041236.

    Proteomic databases

    PaxDbi Q0VCA5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 524683.
    KEGGi bta:524683.

    Organism-specific databases

    CTDi 25939.

    Phylogenomic databases

    eggNOGi COG1078.
    HOGENOMi HOG000264286.
    HOVERGENi HBG054208.
    InParanoidi Q0VCA5.

    Miscellaneous databases

    NextBioi 20874017.

    Family and domain databases

    Gene3Di 1.10.3210.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR013761. SAM/pointed.
    [Graphical view ]
    Pfami PF01966. HD. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal medulla.

    Entry informationi

    Entry nameiSAMH1_BOVIN
    AccessioniPrimary (citable) accession number: Q0VCA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3