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Q0VCA5

- SAMH1_BOVIN

UniProt

Q0VCA5 - SAMH1_BOVIN

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Protein
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Gene
SAMHD1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Host restriction nuclease that blocks early-stage virus replication in dendritic and other myeloid cells. Likewise, suppresses LINE-1 retrotransposon activity. May function by reducing the cellular dNTP levels to levels too low for retroviral reverse transcription to occur. May play a role in mediating proinflammatory responses to TNF-alpha signaling By similarity.

Catalytic activityi

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Enzyme regulationi

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041dGTP 1 By similarity
Binding sitei107 – 1071dGTP 2; via amide nitrogen; shared with neighboring subunit By similarity
Binding sitei137 – 1371Substrate By similarity
Binding sitei152 – 1521Substrate By similarity
Metal bindingi155 – 1551Zinc; via tele nitrogen By similarity
Metal bindingi194 – 1941Zinc; via tele nitrogen By similarity
Metal bindingi195 – 1951Zinc By similarity
Binding sitei198 – 1981Substrate By similarity
Active sitei221 – 2211 By similarity
Metal bindingi300 – 3001Zinc By similarity
Binding sitei304 – 3041Substrate By similarity
Binding sitei308 – 3081Substrate By similarity
Binding sitei322 – 3221dGTP 2 By similarity
Binding sitei347 – 3471dGTP 2 By similarity
Binding sitei355 – 3551Substrate By similarity
Binding sitei365 – 3651dGTP 2; shared with neighboring subunit By similarity
Binding sitei366 – 3661dGTP 2; shared with neighboring subunit By similarity
Binding sitei440 – 4401dGTP 1; shared with neighboring subunit By similarity
Binding sitei444 – 4441dGTP 1; shared with neighboring subunit By similarity
Binding sitei512 – 5121dGTP 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi125 – 1339dGTP By similarity
Nucleotide bindingi125 – 1339dGTP 1 By similarity
Nucleotide bindingi341 – 3433dGTP 2 By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB
  2. dGTP binding Source: UniProtKB
  3. dGTPase activity Source: UniProtKB
  4. nucleic acid binding Source: UniProtKB
  5. phosphoric diester hydrolase activity Source: InterPro
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dATP catabolic process Source: UniProtKB
  2. dGTP catabolic process Source: UniProtKB
  3. defense response to virus Source: UniProtKB
  4. innate immune response Source: UniProtKB-KW
  5. regulation of innate immune response Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
Short name:
dNTPase
Gene namesi
Name:SAMHD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
PRO_0000361968Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei18 – 181Phosphoserine By similarity
Modified residuei21 – 211Phosphothreonine By similarity

Post-translational modificationi

Ubiquitinated and targeted for proteasomal degradation by a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase with the help of the viral accessory protein Vpx By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ0VCA5.

Interactioni

Subunit structurei

Homodimer. Homotetramer; in dGTP-bound form By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000041236.

Structurei

3D structure databases

ProteinModelPortaliQ0VCA5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 10056SAM
Add
BLAST
Domaini152 – 308157HD
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 3047Substrate binding By similarity
Regioni359 – 3646Substrate binding By similarity

Sequence similaritiesi

Belongs to the SAMHD1 family.
Contains 1 HD domain.

Phylogenomic databases

eggNOGiCOG1078.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ0VCA5.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0VCA5-1 [UniParc]FASTAAdd to Basket

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MQSADSQNTP KRPRRDGSPR TPPDSPLADA ETSPSHDLDP DYRTWGPEQV    50
WSFLRRCGFS DSELLKRCRE KRMSGSLLPF PEDLGISSHG KKMKLLNCIQ 100
DTMKVINDPI HGHIEFHPLL MRIIDTPQFQ RLRYIKQLGG GYYVFPGASH 150
NRFEHSLGVG YLAGRLVREL SEKQPELQIS ERDILCVQIA GLCHDLGHGP 200
FSHMFDGRFI PLARPEIKWT HEQGSVMMFE HLINSNGLQD VMKYYGLIPE 250
EDILFIKEQI TGPPESPIKD ASKWLYKGRP KEKSFLYEIV ANKRNGIDVD 300
KWDYFARDCH HLGIQNSFDY KRFLKFARVC EVDNMKHICT REKEVGNLYD 350
MFHTRNCLHR RAYQHKVGNI IDTMITDAFL KADDHIEITG SAGRKYHIST 400
AIDDMEAFTK LTDNIFLEIL YSTDPNLNDA RMILKKIESR NLYKFVGETQ 450
PMIQRIKKEN YEHLPNEVAS AKPSDVELEA ELKAEDLIVD VINMDYGMED 500
KNPIDHVRFY CKSDLSKAVM ITRNQVSQFL PETFAEQLIR VYCKKTDEKT 550
LFAARQHFVH WCLINDFTKP QIKKLPLRKL KKELTTATG 589
Length:589
Mass (Da):68,239
Last modified:September 5, 2006 - v1
Checksum:i260B7E035B2A6F85
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120271 mRNA. Translation: AAI20272.1.
RefSeqiNP_001069329.1. NM_001075861.1.
UniGeneiBt.92388.

Genome annotation databases

GeneIDi524683.
KEGGibta:524683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120271 mRNA. Translation: AAI20272.1 .
RefSeqi NP_001069329.1. NM_001075861.1.
UniGenei Bt.92388.

3D structure databases

ProteinModelPortali Q0VCA5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000041236.

Proteomic databases

PaxDbi Q0VCA5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 524683.
KEGGi bta:524683.

Organism-specific databases

CTDi 25939.

Phylogenomic databases

eggNOGi COG1078.
HOGENOMi HOG000264286.
HOVERGENi HBG054208.
InParanoidi Q0VCA5.

Miscellaneous databases

NextBioi 20874017.

Family and domain databases

Gene3Di 1.10.3210.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013761. SAM/pointed.
[Graphical view ]
Pfami PF01966. HD. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal medulla.

Entry informationi

Entry nameiSAMH1_BOVIN
AccessioniPrimary (citable) accession number: Q0VCA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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