Reviewed, UniProtKB/Swiss-Prot Q0VC74 (TMLH_BOVIN)

Names and origin

Protein names

Recommended name:
    Trimethyllysine dioxygenase, mitochondrial
    EC=1.14.11.8
Alternative name(s):
    Epsilon-trimethyllysine 2-oxoglutarate dioxygenase
    TML-alpha-ketoglutarate dioxygenase
      Short name=TML dioxygenase
      Short name=TMLD
    TML hydroxylase

Gene names

Name:

TMLHE

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML) By similarity.
Catalytic activity	N6,N6,N(6)-trimethyl-L-lysine + 2-oxoglutarate + O2 = 3-hydroxy-N6,N6,N(6)-trimethyl-L-lysine + succinate + CO2.
Cofactor	Iron By similarity. Ascorbate By similarity.
Pathway	Amine and polyamine biosynthesis; carnitine biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the gamma-BBH/TMLD family.

Ontologies

Keywords
Biological process	Carnitine biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	carnitine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW trimethyllysine dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 15	15	Mitochondrion By similarity
	Chain	16 – 421	406	Trimethyllysine dioxygenase, mitochondrial		PRO_0000260156
Sites
	Binding site	242	1	2-oxoglutarate Probable
	Binding site	244	1	2-oxoglutarate Probable
	Binding site	389	1	2-oxoglutarate Probable
Amino acid modifications
	Modified residue	236	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VC74-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6FADE146279CA760	FASTA	421	49,837
	10 20 30 40 50 60 MWCHRLSHLQ SRLQDLLRGR VTRWALQQSN FKSLFPLAIY WHHTASKSLN CVWQQHEDHF 70 80 90 100 110 120 ELQYANNVMR FDYVWLRDHC RSASCYNSKT HQRSLDTASV DLCIQPQTIH LDETTLFFTW 130 140 150 160 170 180 PDGHVTRYDL DWLMKNSYEG QKQKVIQPRI LWNAEIYQQA QVPAVDFQTF LETKEGLKNF 190 200 210 220 230 240 LQNFLLYGIA FVENVPPTQK HTEKLAERIS LIRETIYGRM WFFTSDFSRG DTAYTKLALD 250 260 270 280 290 300 RHTDTTYFQE PCGIQVFHCL KHEGTGGRTL LVDGFYAAEQ VLQKAPEEFE LLSKVPLKHE 310 320 330 340 350 360 YIENVGECQN HMIGVGPVLN IYPWNKELYL IRYNNYDRAV INTVPYDVVH RWYTAHRTLT 370 380 390 400 410 420 RELRRPENEF WVKLKPGKVL FIDNWRVLHG RESFTGYRQL CGCYLTRDDV LNTARLLGLQ A

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC120318 mRNA. Translation: AAI20319.1.
IPI	IPI00721209.
RefSeq	NP_001069532.1.
UniGene	Bt.21098
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000015469; ENSBTAP00000015469; ENSBTAG00000011648; Bos taurus. [Genome view]
GeneID	535630.
KEGG	bta:535630.
Organism-specific databases
CTD	535630.
Phylogenomic databases
eggNOG	maNOG08663.
HOVERGEN	Q0VC74.
PhylomeDB	Q0VC74.
Enzyme and pathway databases
BRENDA	1.14.11.8. 251.
Family and domain databases
InterPro	IPR003819. Taurine_dOase. IPR012776. Trimethyllysine_dOase. [Graphical view]
PANTHER	PTHR10696:SF2. tMLys_dOase. 1 hit.
Pfam	PF02668. TauD. 1 hit. [Graphical view]
TIGRFAMs	TIGR02410. carnitine_TMLD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TMLH_BOVIN

Accession

Primary (citable) accession number: Q0VC74

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	September 5, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)