Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q0VC53

- DOHH_BOVIN

UniProt

Q0VC53 - DOHH_BOVIN

Protein

Deoxyhypusine hydroxylase

Gene

DOHH

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.1 PublicationUniRule annotation

    Catalytic activityi

    [eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

    Cofactori

    Binds 2 Fe2+ ions per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Iron 1UniRule annotation
    Metal bindingi57 – 571Iron 1UniRule annotation
    Metal bindingi89 – 891Iron 1UniRule annotation
    Metal bindingi90 – 901Iron 1UniRule annotation
    Metal bindingi208 – 2081Iron 2UniRule annotation
    Metal bindingi209 – 2091Iron 2UniRule annotation
    Metal bindingi241 – 2411Iron 2UniRule annotation
    Metal bindingi242 – 2421Iron 2UniRule annotation

    GO - Molecular functioni

    1. deoxyhypusine monooxygenase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Hypusine biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_205598. Hypusine synthesis from eIF5A-lysine.
    UniPathwayiUPA00354.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
    Short name:
    DOHHUniRule annotation
    Alternative name(s):
    Deoxyhypusine dioxygenaseUniRule annotation
    Deoxyhypusine monooxygenaseUniRule annotation
    Gene namesi
    Name:DOHHUniRule annotation
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 7

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi57 – 571E → G: Severe reduction in activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 303303Deoxyhypusine hydroxylasePRO_0000326490Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ0VC53.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000006935.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VC53.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati23 – 4927HEAT-like PBS-type 1Add
    BLAST
    Repeati54 – 8027HEAT-like PBS-type 2Add
    BLAST
    Repeati87 – 11327HEAT-like PBS-type 3Add
    BLAST
    Repeati175 – 20127HEAT-like PBS-type 4Add
    BLAST
    Repeati206 – 23227HEAT-like PBS-type 5Add
    BLAST
    Repeati239 – 26527HEAT-like PBS-type 6Add
    BLAST

    Sequence similaritiesi

    Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
    Contains 6 HEAT-like PBS-type repeats.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1413.
    GeneTreeiENSGT00500000044957.
    HOGENOMiHOG000248665.
    HOVERGENiHBG081460.
    InParanoidiQ0VC53.
    KOiK06072.
    OMAiRESCQVA.
    OrthoDBiEOG75TMCH.
    TreeFamiTF105626.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR027517. Deoxyhypusine_hydroxylase.
    IPR021133. HEAT_type_2.
    IPR004155. PBS_lyase_HEAT.
    [Graphical view]
    SMARTiSM00567. EZ_HEAT. 6 hits.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS50077. HEAT_REPEAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0VC53-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTEQEVEAV GQTLVDPGQP LQARFRALFT LRGLGGPVAI SWISRAFDDD    50
    SALLKHELAY CLGQMQDRRA IPVLLDVLRD TRQEPMVRHE AGEALGAIGD 100
    PEVLEILKQY STDPVVEVAE TCQLAVRRLE WLQQHGGESA VRGPYLSVDP 150
    APPAEERDLG QLREALLDEA RPLFDRYRAM FALRDAGGKE AALALAEGLR 200
    CGSALFRHEI GYVLGQMQHE AAVPQLAAAL AQPTENPMVR HECAEALGAI 250
    ARPACLAALR AHVADPERVV RESCEVALDM YEYETGSTFQ YADGLERLRS 300
    PLS 303
    Length:303
    Mass (Da):33,261
    Last modified:September 5, 2006 - v1
    Checksum:i4487BA5BDFC61AB9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti266 – 2661P → S in ABL86660. (PubMed:17391984)Curated
    Sequence conflicti266 – 2661P → S in ABL86661. (PubMed:17391984)Curated
    Sequence conflicti266 – 2661P → S in ABL86662. (PubMed:17391984)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ990881 mRNA. Translation: ABL86660.1.
    DQ990882 mRNA. Translation: ABL86661.1.
    DQ990883 mRNA. Translation: ABL86662.1.
    BC120351 mRNA. Translation: AAI20352.1.
    RefSeqiNP_001069354.1. NM_001075886.1.
    XP_005209005.1. XM_005208948.1.
    UniGeneiBt.10480.

    Genome annotation databases

    EnsembliENSBTAT00000006935; ENSBTAP00000006935; ENSBTAG00000005272.
    GeneIDi526521.
    KEGGibta:526521.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ990881 mRNA. Translation: ABL86660.1 .
    DQ990882 mRNA. Translation: ABL86661.1 .
    DQ990883 mRNA. Translation: ABL86662.1 .
    BC120351 mRNA. Translation: AAI20352.1 .
    RefSeqi NP_001069354.1. NM_001075886.1.
    XP_005209005.1. XM_005208948.1.
    UniGenei Bt.10480.

    3D structure databases

    ProteinModelPortali Q0VC53.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000006935.

    Proteomic databases

    PRIDEi Q0VC53.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000006935 ; ENSBTAP00000006935 ; ENSBTAG00000005272 .
    GeneIDi 526521.
    KEGGi bta:526521.

    Organism-specific databases

    CTDi 83475.

    Phylogenomic databases

    eggNOGi COG1413.
    GeneTreei ENSGT00500000044957.
    HOGENOMi HOG000248665.
    HOVERGENi HBG081460.
    InParanoidi Q0VC53.
    KOi K06072.
    OMAi RESCQVA.
    OrthoDBi EOG75TMCH.
    TreeFami TF105626.

    Enzyme and pathway databases

    UniPathwayi UPA00354 .
    Reactomei REACT_205598. Hypusine synthesis from eIF5A-lysine.

    Miscellaneous databases

    NextBioi 20874385.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    HAMAPi MF_03101. Deoxyhypusine_hydroxylase.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR027517. Deoxyhypusine_hydroxylase.
    IPR021133. HEAT_type_2.
    IPR004155. PBS_lyase_HEAT.
    [Graphical view ]
    SMARTi SM00567. EZ_HEAT. 6 hits.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS50077. HEAT_REPEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional expression of bovine deoxyhypusine hydroxylase cDNA and homologs."
      Huang J.-K., Cui Y., Chen C.-H., Clampitt D., Lin C.-T., Wen L.
      Protein Expr. Purif. 54:126-133(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF GLU-57.
      Tissue: Brain.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal skin.

    Entry informationi

    Entry nameiDOHH_BOVIN
    AccessioniPrimary (citable) accession number: Q0VC53
    Secondary accession number(s): A5H2K6, A5H2K8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3