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Protein

Deoxyhypusine hydroxylase

Gene

DOHH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation1 Publication

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Iron 1UniRule annotation1
Metal bindingi57Iron 1UniRule annotation1
Metal bindingi89Iron 1UniRule annotation1
Metal bindingi90Iron 1UniRule annotation1
Metal bindingi208Iron 2UniRule annotation1
Metal bindingi209Iron 2UniRule annotation1
Metal bindingi241Iron 2UniRule annotation1
Metal bindingi242Iron 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.99.29. 908.
ReactomeiR-BTA-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:DOHHUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57E → G: Severe reduction in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264901 – 303Deoxyhypusine hydroxylaseAdd BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ0VC53.
PRIDEiQ0VC53.

Expressioni

Gene expression databases

BgeeiENSBTAG00000005272.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006935.

Structurei

3D structure databases

ProteinModelPortaliQ0VC53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 49HEAT-like PBS-type 1Add BLAST27
Repeati54 – 80HEAT-like PBS-type 2Add BLAST27
Repeati87 – 113HEAT-like PBS-type 3Add BLAST27
Repeati175 – 201HEAT-like PBS-type 4Add BLAST27
Repeati206 – 232HEAT-like PBS-type 5Add BLAST27
Repeati239 – 265HEAT-like PBS-type 6Add BLAST27

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 6 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0567. Eukaryota.
COG1413. LUCA.
GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
HOVERGENiHBG081460.
InParanoidiQ0VC53.
KOiK06072.
OMAiAIDMWEY.
OrthoDBiEOG091G0E2R.
TreeFamiTF105626.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0VC53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTEQEVEAV GQTLVDPGQP LQARFRALFT LRGLGGPVAI SWISRAFDDD
60 70 80 90 100
SALLKHELAY CLGQMQDRRA IPVLLDVLRD TRQEPMVRHE AGEALGAIGD
110 120 130 140 150
PEVLEILKQY STDPVVEVAE TCQLAVRRLE WLQQHGGESA VRGPYLSVDP
160 170 180 190 200
APPAEERDLG QLREALLDEA RPLFDRYRAM FALRDAGGKE AALALAEGLR
210 220 230 240 250
CGSALFRHEI GYVLGQMQHE AAVPQLAAAL AQPTENPMVR HECAEALGAI
260 270 280 290 300
ARPACLAALR AHVADPERVV RESCEVALDM YEYETGSTFQ YADGLERLRS

PLS
Length:303
Mass (Da):33,261
Last modified:September 5, 2006 - v1
Checksum:i4487BA5BDFC61AB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266P → S in ABL86660 (PubMed:17391984).Curated1
Sequence conflicti266P → S in ABL86661 (PubMed:17391984).Curated1
Sequence conflicti266P → S in ABL86662 (PubMed:17391984).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ990881 mRNA. Translation: ABL86660.1.
DQ990882 mRNA. Translation: ABL86661.1.
DQ990883 mRNA. Translation: ABL86662.1.
BC120351 mRNA. Translation: AAI20352.1.
RefSeqiNP_001069354.1. NM_001075886.1.
XP_005209005.1. XM_005208948.2.
XP_010805282.1. XM_010806980.1.
UniGeneiBt.10480.

Genome annotation databases

EnsembliENSBTAT00000006935; ENSBTAP00000006935; ENSBTAG00000005272.
GeneIDi526521.
KEGGibta:526521.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ990881 mRNA. Translation: ABL86660.1.
DQ990882 mRNA. Translation: ABL86661.1.
DQ990883 mRNA. Translation: ABL86662.1.
BC120351 mRNA. Translation: AAI20352.1.
RefSeqiNP_001069354.1. NM_001075886.1.
XP_005209005.1. XM_005208948.2.
XP_010805282.1. XM_010806980.1.
UniGeneiBt.10480.

3D structure databases

ProteinModelPortaliQ0VC53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006935.

Proteomic databases

PaxDbiQ0VC53.
PRIDEiQ0VC53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000006935; ENSBTAP00000006935; ENSBTAG00000005272.
GeneIDi526521.
KEGGibta:526521.

Organism-specific databases

CTDi83475.

Phylogenomic databases

eggNOGiKOG0567. Eukaryota.
COG1413. LUCA.
GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
HOVERGENiHBG081460.
InParanoidiQ0VC53.
KOiK06072.
OMAiAIDMWEY.
OrthoDBiEOG091G0E2R.
TreeFamiTF105626.

Enzyme and pathway databases

UniPathwayiUPA00354.
BRENDAi1.14.99.29. 908.
ReactomeiR-BTA-204626. Hypusine synthesis from eIF5A-lysine.

Gene expression databases

BgeeiENSBTAG00000005272.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDOHH_BOVIN
AccessioniPrimary (citable) accession number: Q0VC53
Secondary accession number(s): A5H2K6, A5H2K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 5, 2006
Last modified: September 7, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.