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Q0VC53

- DOHH_BOVIN

UniProt

Q0VC53 - DOHH_BOVIN

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Protein

Deoxyhypusine hydroxylase

Gene

DOHH

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.1 PublicationUniRule annotation

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron 1UniRule annotation
Metal bindingi57 – 571Iron 1UniRule annotation
Metal bindingi89 – 891Iron 1UniRule annotation
Metal bindingi90 – 901Iron 1UniRule annotation
Metal bindingi208 – 2081Iron 2UniRule annotation
Metal bindingi209 – 2091Iron 2UniRule annotation
Metal bindingi241 – 2411Iron 2UniRule annotation
Metal bindingi242 – 2421Iron 2UniRule annotation

GO - Molecular functioni

  1. deoxyhypusine monooxygenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_205598. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:DOHHUniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 7

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi57 – 571E → G: Severe reduction in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Deoxyhypusine hydroxylasePRO_0000326490Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ0VC53.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006935.

Structurei

3D structure databases

ProteinModelPortaliQ0VC53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati23 – 4927HEAT-like PBS-type 1Add
BLAST
Repeati54 – 8027HEAT-like PBS-type 2Add
BLAST
Repeati87 – 11327HEAT-like PBS-type 3Add
BLAST
Repeati175 – 20127HEAT-like PBS-type 4Add
BLAST
Repeati206 – 23227HEAT-like PBS-type 5Add
BLAST
Repeati239 – 26527HEAT-like PBS-type 6Add
BLAST

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 6 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1413.
GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
HOVERGENiHBG081460.
InParanoidiQ0VC53.
KOiK06072.
OMAiRESCQVA.
OrthoDBiEOG75TMCH.
TreeFamiTF105626.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0VC53 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTEQEVEAV GQTLVDPGQP LQARFRALFT LRGLGGPVAI SWISRAFDDD
60 70 80 90 100
SALLKHELAY CLGQMQDRRA IPVLLDVLRD TRQEPMVRHE AGEALGAIGD
110 120 130 140 150
PEVLEILKQY STDPVVEVAE TCQLAVRRLE WLQQHGGESA VRGPYLSVDP
160 170 180 190 200
APPAEERDLG QLREALLDEA RPLFDRYRAM FALRDAGGKE AALALAEGLR
210 220 230 240 250
CGSALFRHEI GYVLGQMQHE AAVPQLAAAL AQPTENPMVR HECAEALGAI
260 270 280 290 300
ARPACLAALR AHVADPERVV RESCEVALDM YEYETGSTFQ YADGLERLRS

PLS
Length:303
Mass (Da):33,261
Last modified:September 5, 2006 - v1
Checksum:i4487BA5BDFC61AB9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti266 – 2661P → S in ABL86660. (PubMed:17391984)Curated
Sequence conflicti266 – 2661P → S in ABL86661. (PubMed:17391984)Curated
Sequence conflicti266 – 2661P → S in ABL86662. (PubMed:17391984)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ990881 mRNA. Translation: ABL86660.1.
DQ990882 mRNA. Translation: ABL86661.1.
DQ990883 mRNA. Translation: ABL86662.1.
BC120351 mRNA. Translation: AAI20352.1.
RefSeqiNP_001069354.1. NM_001075886.1.
XP_005209005.1. XM_005208948.1.
UniGeneiBt.10480.

Genome annotation databases

EnsembliENSBTAT00000006935; ENSBTAP00000006935; ENSBTAG00000005272.
GeneIDi526521.
KEGGibta:526521.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ990881 mRNA. Translation: ABL86660.1 .
DQ990882 mRNA. Translation: ABL86661.1 .
DQ990883 mRNA. Translation: ABL86662.1 .
BC120351 mRNA. Translation: AAI20352.1 .
RefSeqi NP_001069354.1. NM_001075886.1.
XP_005209005.1. XM_005208948.1.
UniGenei Bt.10480.

3D structure databases

ProteinModelPortali Q0VC53.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000006935.

Proteomic databases

PRIDEi Q0VC53.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000006935 ; ENSBTAP00000006935 ; ENSBTAG00000005272 .
GeneIDi 526521.
KEGGi bta:526521.

Organism-specific databases

CTDi 83475.

Phylogenomic databases

eggNOGi COG1413.
GeneTreei ENSGT00500000044957.
HOGENOMi HOG000248665.
HOVERGENi HBG081460.
InParanoidi Q0VC53.
KOi K06072.
OMAi RESCQVA.
OrthoDBi EOG75TMCH.
TreeFami TF105626.

Enzyme and pathway databases

UniPathwayi UPA00354 .
Reactomei REACT_205598. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

NextBioi 20874385.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
HAMAPi MF_03101. Deoxyhypusine_hydroxylase.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR021133. HEAT_type_2.
IPR004155. PBS_lyase_HEAT.
[Graphical view ]
SMARTi SM00567. EZ_HEAT. 6 hits.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS50077. HEAT_REPEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional expression of bovine deoxyhypusine hydroxylase cDNA and homologs."
    Huang J.-K., Cui Y., Chen C.-H., Clampitt D., Lin C.-T., Wen L.
    Protein Expr. Purif. 54:126-133(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF GLU-57.
    Tissue: Brain.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiDOHH_BOVIN
AccessioniPrimary (citable) accession number: Q0VC53
Secondary accession number(s): A5H2K6, A5H2K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: September 5, 2006
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3