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Q0VBZ0

- CSK_BOVIN

UniProt

Q0VBZ0 - CSK_BOVIN

Protein

Tyrosine-protein kinase CSK

Gene

CSK

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei222 – 2221ATPPROSITE-ProRule annotation
    Active sitei314 – 3141Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi201 – 2099ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. immune system process Source: UniProtKB-KW
    2. negative regulation of cell proliferation Source: Ensembl
    3. negative regulation of Golgi to plasma membrane protein transport Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_206808. Integrin alphaIIb beta3 signaling.
    REACT_212961. Phosphorylation of CD3 and TCR zeta chains.
    REACT_213223. PD-1 signaling.
    REACT_222082. GAB1 signalosome.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase CSK (EC:2.7.10.2)
    Alternative name(s):
    C-Src kinase
    Gene namesi
    Name:CSK
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 21

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Mainly cytoplasmic, also present in lipid rafts.By similarity

    GO - Cellular componenti

    1. cell-cell junction Source: Ensembl
    2. centrosome Source: Ensembl
    3. Golgi apparatus Source: Ensembl
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 450449Tyrosine-protein kinase CSKPRO_0000260260Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei304 – 3041PhosphotyrosineBy similarity
    Modified residuei364 – 3641Phosphoserine; by PKABy similarity
    Modified residuei416 – 4161PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ0VBZ0.
    PRIDEiQ0VBZ0.

    Interactioni

    Subunit structurei

    Homodimer (via SH3-domain). Interacts with PTPN8. Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases. Interacts with SRCIN1. Interacts with RHOH. Interacts (via SH2 domain) with SCIMP By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000028559.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0VBZ0.
    SMRiQ0VBZ0. Positions 6-450.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 7062SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini82 – 17190SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini195 – 449255Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni9 – 7062Interaction with PTPN8By similarityAdd
    BLAST

    Domaini

    The architecture of this protein is similar to that of Src-family kinases (SFKs) with one N-terminal SH3 domain, one SH2 domain, and a C-terminal kinase domain.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117264.
    HOGENOMiHOG000233858.
    HOVERGENiHBG008761.
    InParanoidiQ0VBZ0.
    KOiK05728.
    OMAiERLLCPP.
    OrthoDBiEOG7SV0V5.
    TreeFamiTF351634.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q0VBZ0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA    50
    KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLCPPE 100
    TGLFLVREST NYPGDYTLCV SCDGKVEHYR IMYHASKLSI DEEVYFENLM 150
    QLVEHYTSDA DGLCTRLIKP KVMEGTVAAQ DEFFRSGWAL NMKDLKLLQT 200
    IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM TQLRHSNLVQ 250
    LLGVIVEEKS GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 300
    AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV 350
    KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE 400
    KGYKMDAPDG CPPAVYEVMK NCWHLDAATR PSFLQLREQL ERIKTHELHL 450
    Length:450
    Mass (Da):50,633
    Last modified:September 5, 2006 - v1
    Checksum:iB42BD31C645783D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120437 mRNA. Translation: AAI20438.1.
    RefSeqiNP_001068865.1. NM_001075397.1.
    UniGeneiBt.20371.

    Genome annotation databases

    EnsembliENSBTAT00000028559; ENSBTAP00000028559; ENSBTAG00000021424.
    GeneIDi509246.
    KEGGibta:509246.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC120437 mRNA. Translation: AAI20438.1 .
    RefSeqi NP_001068865.1. NM_001075397.1.
    UniGenei Bt.20371.

    3D structure databases

    ProteinModelPortali Q0VBZ0.
    SMRi Q0VBZ0. Positions 6-450.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000028559.

    Proteomic databases

    PaxDbi Q0VBZ0.
    PRIDEi Q0VBZ0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000028559 ; ENSBTAP00000028559 ; ENSBTAG00000021424 .
    GeneIDi 509246.
    KEGGi bta:509246.

    Organism-specific databases

    CTDi 1445.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117264.
    HOGENOMi HOG000233858.
    HOVERGENi HBG008761.
    InParanoidi Q0VBZ0.
    KOi K05728.
    OMAi ERLLCPP.
    OrthoDBi EOG7SV0V5.
    TreeFami TF351634.

    Enzyme and pathway databases

    Reactomei REACT_206808. Integrin alphaIIb beta3 signaling.
    REACT_212961. Phosphorylation of CD3 and TCR zeta chains.
    REACT_213223. PD-1 signaling.
    REACT_222082. GAB1 signalosome.

    Miscellaneous databases

    NextBioi 20868890.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Basal ganglia.

    Entry informationi

    Entry nameiCSK_BOVIN
    AccessioniPrimary (citable) accession number: Q0VBZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3