Reviewed, UniProtKB/Swiss-Prot Q0VBZ0 (CSK_BOVIN)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase CSK
    EC=2.7.10.2
Alternative name(s):
    C-SRC kinase

Gene names

Name:

CSK

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with PTPN8. Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1 By similarity.
Subcellular location	Cytoplasm By similarity. Cell membrane By similarity. Note: Mainly cytoplasmic, also present in lipid rafts By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Tyrosine-protein kinase CSK		PRO_0000260260
Regions
	Domain	9 – 70	62	SH3
	Domain	82 – 171	90	SH2
	Domain	195 – 449	255	Protein kinase
	Nucleotide binding	201 – 209	9	ATP By similarity
	Region	9 – 70	62	Interaction with PTPN8 By similarity
Sites
	Active site	314	1	Proton acceptor By similarity
	Binding site	222	1	ATP By similarity
Amino acid modifications
	Modified residue	416	1	Phosphotyrosine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0VBZ0-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: B42BD31C645783D0	FASTA	450	50,633
	10 20 30 40 50 60 MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII 70 80 90 100 110 120 PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLCPPE TGLFLVREST NYPGDYTLCV 130 140 150 160 170 180 SCDGKVEHYR IMYHASKLSI DEEVYFENLM QLVEHYTSDA DGLCTRLIKP KVMEGTVAAQ 190 200 210 220 230 240 DEFFRSGWAL NMKDLKLLQT IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM 250 260 270 280 290 300 TQLRHSNLVQ LLGVIVEEKS GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 310 320 330 340 350 360 AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE 370 380 390 400 410 420 KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYEVMK 430 440 450 NCWHLDAATR PSFLQLREQL ERIKTHELHL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC120437 mRNA. Translation: AAI20438.1.
IPI	IPI00698198.
RefSeq	NP_001068865.1.
UniGene	Bt.20371
3D structure databases
SMR	Q0VBZ0. Positions 4-450.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000021424. Bos taurus. [Contig view]
GeneID	509246.
KEGG	bta:509246.
Phylogenomic databases
HOVERGEN	Q0VBZ0.
OMA	Q0VBZ0. QDLPFCK.
Enzyme and pathway databases
BRENDA	2.7.10.2. 251.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR015778. Tyrosine_Protein_Kinase_Csk. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256:SF264. Csk_ProtTyrKin. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. PD000093. SH2. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CSK_BOVIN

Accession

Primary (citable) accession number: Q0VBZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)