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Q0VBZ0

- CSK_BOVIN

UniProt

Q0VBZ0 - CSK_BOVIN

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Protein
Tyrosine-protein kinase CSK
Gene
CSK
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK By similarity.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei222 – 2221ATP By similarity
Active sitei314 – 3141Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi201 – 2099ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. immune system process Source: UniProtKB-KW
  2. negative regulation of Golgi to plasma membrane protein transport Source: Ensembl
  3. negative regulation of cell proliferation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Adaptive immunity, Immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_206808. Integrin alphaIIb beta3 signaling.
REACT_212961. Phosphorylation of CD3 and TCR zeta chains.
REACT_213223. PD-1 signaling.
REACT_222082. GAB1 signalosome.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase CSK (EC:2.7.10.2)
Alternative name(s):
C-Src kinase
Gene namesi
Name:CSK
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 21

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Mainly cytoplasmic, also present in lipid rafts By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. cell-cell junction Source: Ensembl
  3. centrosome Source: Ensembl
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 450449Tyrosine-protein kinase CSK
PRO_0000260260Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei304 – 3041Phosphotyrosine By similarity
Modified residuei364 – 3641Phosphoserine; by PKA By similarity
Modified residuei416 – 4161Phosphotyrosine By similarity

Post-translational modificationi

Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ0VBZ0.
PRIDEiQ0VBZ0.

Interactioni

Subunit structurei

Homodimer (via SH3-domain). Interacts with PTPN8. Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases. Interacts with SRCIN1. Interacts with RHOH. Interacts (via SH2 domain) with SCIMP By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028559.

Structurei

3D structure databases

ProteinModelPortaliQ0VBZ0.
SMRiQ0VBZ0. Positions 6-450.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 7062SH3
Add
BLAST
Domaini82 – 17190SH2
Add
BLAST
Domaini195 – 449255Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 7062Interaction with PTPN8 By similarity
Add
BLAST

Domaini

The architecture of this protein is similar to that of Src-family kinases (SFKs) with one N-terminal SH3 domain, one SH2 domain, and a C-terminal kinase domain By similarity.

Sequence similaritiesi

Contains 1 SH2 domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117264.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ0VBZ0.
KOiK05728.
OMAiERLLCPP.
OrthoDBiEOG7SV0V5.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0VBZ0-1 [UniParc]FASTAAdd to Basket

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MSAIQAAWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA    50
KNKVGREGII PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLCPPE 100
TGLFLVREST NYPGDYTLCV SCDGKVEHYR IMYHASKLSI DEEVYFENLM 150
QLVEHYTSDA DGLCTRLIKP KVMEGTVAAQ DEFFRSGWAL NMKDLKLLQT 200
IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM TQLRHSNLVQ 250
LLGVIVEEKS GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 300
AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV 350
KWTAPEALRE KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE 400
KGYKMDAPDG CPPAVYEVMK NCWHLDAATR PSFLQLREQL ERIKTHELHL 450
Length:450
Mass (Da):50,633
Last modified:September 5, 2006 - v1
Checksum:iB42BD31C645783D0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120437 mRNA. Translation: AAI20438.1.
RefSeqiNP_001068865.1. NM_001075397.1.
UniGeneiBt.20371.

Genome annotation databases

EnsembliENSBTAT00000028559; ENSBTAP00000028559; ENSBTAG00000021424.
GeneIDi509246.
KEGGibta:509246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC120437 mRNA. Translation: AAI20438.1 .
RefSeqi NP_001068865.1. NM_001075397.1.
UniGenei Bt.20371.

3D structure databases

ProteinModelPortali Q0VBZ0.
SMRi Q0VBZ0. Positions 6-450.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000028559.

Proteomic databases

PaxDbi Q0VBZ0.
PRIDEi Q0VBZ0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000028559 ; ENSBTAP00000028559 ; ENSBTAG00000021424 .
GeneIDi 509246.
KEGGi bta:509246.

Organism-specific databases

CTDi 1445.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117264.
HOGENOMi HOG000233858.
HOVERGENi HBG008761.
InParanoidi Q0VBZ0.
KOi K05728.
OMAi ERLLCPP.
OrthoDBi EOG7SV0V5.
TreeFami TF351634.

Enzyme and pathway databases

Reactomei REACT_206808. Integrin alphaIIb beta3 signaling.
REACT_212961. Phosphorylation of CD3 and TCR zeta chains.
REACT_213223. PD-1 signaling.
REACT_222082. GAB1 signalosome.

Miscellaneous databases

NextBioi 20868890.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.

Entry informationi

Entry nameiCSK_BOVIN
AccessioniPrimary (citable) accession number: Q0VBZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: September 5, 2006
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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