SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q0VBL6

- HIF3A_MOUSE

UniProt

Q0VBL6 - HIF3A_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Hypoxia-inducible factor 3-alpha
Gene
Hif3a, Mop7
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 2 has a dominant-negative function of inactivating HIF1-mediated transcription. Isoform 2 attenuates the binding of HIF1 to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 2, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 2 increases and thereby secures an inhibition of HIF1A activity By similarity. May be a negative regulator of hypoxia-inducible gene expression in the kidney. May be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.1 Publication

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding transcription factor activity Source: MGI
  3. signal transducer activity Source: InterPro
  4. transcription coactivator activity Source: UniProtKB
  5. transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. response to hypoxia Source: MGI
  2. transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.
REACT_199108. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxia-inducible factor 3-alpha
Short name:
HIF-3-alpha
Short name:
HIF3-alpha
Alternative name(s):
Basic-helix-loop-helix-PAS protein MOP7
HIF3-alpha-1
Inhibitory PAS domain protein
Short name:
IPAS
Member of PAS protein 7
Gene namesi
Name:Hif3a
Synonyms:Mop7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1859778. Hif3a.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Hypoxia-inducible factor 3-alpha
PRO_0000284415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki463 – 463Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei487 – 48714-hydroxyproline By similarity
Cross-linki565 – 565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Post-translational modificationi

In normoxia, hydroxylated on Pro-487 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation By similarity.

Keywords - PTMi

Hydroxylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiQ0VBL6.

PTM databases

PhosphoSiteiQ0VBL6.

Expressioni

Tissue specificityi

Expressed in lung, brain, heart and kidney. Isoform 2 is expressed predominantly in the eye and at lower levels in the cerebellum and the cerebrum. Highly expressed in the epithelial cell layer of the cornea with lower expression in the layers of ganglion cells, inner nuclear cells, and rods and cones of the retina. In the cerebellum expressed only the Purkinje cell layer.2 Publications

Inductioni

Strongly induced by hypoxia By similarity.

Gene expression databases

ArrayExpressiQ0VBL6.
BgeeiQ0VBL6.
CleanExiMM_HIF3A.
GenevestigatoriQ0VBL6.

Interactioni

Subunit structurei

Heterodimerizes with ARNT. Interacts via the oxygen-dependent degradation domain (ODD) with the beta domain of VHL.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ0VBL6.
SMRiQ0VBL6. Positions 10-340.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 6554bHLH
Add
BLAST
Domaini80 – 15071PAS 1
Add
BLAST
Domaini225 – 29571PAS 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni448 – 581134ODD
Add
BLAST
Regioni450 – 50152NTAD
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi414 – 4185LRRLL
Motifi485 – 4928LAPYISMD

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG288942.
GeneTreeiENSGT00730000110711.
HOGENOMiHOG000234306.
HOVERGENiHBG060456.
KOiK09096.

Family and domain databases

InterProiIPR011598. bHLH_dom.
IPR021537. HIF_alpha_subunit.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view]
PfamiPF11413. HIF-1. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q0VBL6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDWDQDRSNT ELRKEKSRDA ARSRRSQETE VLYQLAHTLP FARGVSAHLD    50
KASIMRLTIS YLRMHRLCAA GEWNQVEKGG EPLDACYLKA LEGFVMVLTA 100
EGDMAYLSEN VSKHLGLSQL ELIGHSIFDF IHPCDQEELQ DALTPRPNLS 150
KKKLEAPTER HFSLRMKSTL TSRGRTLNLK AATWKVLHCS GHMRAYKPPA 200
QTSPAGSPRS EPPLQCLVLI CEAIPHPASL EPPLGRGAFL SRHSLDMKFT 250
YCDERIAEVA GYSPDDLIGC SAYEYIHALD SDAVSRSIHT LLSKGQAVTG 300
QYRFLARTGG YLWTQTQATV VSGGRGPQSE SIICVHFLIS RVEETGVVLS 350
LEQTEQHTRR PPRLSASSQK GIPGNSVDSP APRILAFLHP PALSEASLAA 400
DPRRFCSPDL RRLMAPILDG PPPAATPSTP QATRRPQSPL PADLPDKLTV 450
GLENAHRLST AQKNKTVETD LDIAQDPDTL DLEMLAPYIS MDDDFQLNSS 500
EQLPKVHRRP PRVARRPRAR SFHGLSPPIP EPSLLPRWGS DPRLNCSSPS 550
RGDRPTASLM PGTRKRALAQ SSEDKGLELL ETKPPKRSPR LEPGSFLLPP 600
LSLSFLLQGR QLPGNQQDPR APLVHSHEPL GLAPSLLSLC QHEETVQPRN 650
RFPPAAGLGQ TH 662
Length:662
Mass (Da):73,044
Last modified:July 27, 2011 - v2
Checksum:iD05E2B9CB2B63E4E
GO
Isoform 2 (identifier: Q0VBL6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MDWDQD → MALGLQRV
     72-119: EWNQVEKGGE...NVSKHLGLSQ → GKRGRATGRL...HNPTPGTNFS
     226-291: HPASLEPPLG...DAVSRSIHTL → QLPFHDGATL...WALNRKNCPG
     292-662: Missing.

Show »
Length:307
Mass (Da):33,990
Checksum:iD89D3B225C9C3967
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66MDWDQD → MALGLQRV in isoform 2.
VSP_024528
Alternative sequencei72 – 11948EWNQV…LGLSQ → GKRGRATGRLLPEGPGGFRH GTHRRGRHGLPVGKCQQAPG PQSVDLCSSSLIHNPTPGTN FS in isoform 2.
VSP_024529Add
BLAST
Alternative sequencei226 – 29166HPASL…SIHTL → QLPFHDGATLGLPQEKTPIS TLFTPLWKALLCLVKRWPVQ VLQGKGTESSLPSWVLWALN RKNCPG in isoform 2.
VSP_024530Add
BLAST
Alternative sequencei292 – 662371Missing in isoform 2.
VSP_024531Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti345 – 3451T → N in BAE25907. 1 Publication
Sequence conflicti449 – 4491T → A in AAC72734. 1 Publication
Sequence conflicti449 – 4491T → A in AAF21782. 1 Publication
Sequence conflicti449 – 4491T → A in AAI20588. 1 Publication
Sequence conflicti477 – 4771P → S in AAC72734. 1 Publication
Sequence conflicti477 – 4771P → S in AAF21782. 1 Publication
Sequence conflicti477 – 4771P → S in AAI20588. 1 Publication
Sequence conflicti484 – 4841M → I in BAE25907. 1 Publication
Sequence conflicti582 – 5821T → I in AAC72734. 1 Publication
Sequence conflicti582 – 5821T → I in AAF21782. 1 Publication
Sequence conflicti613 – 6131P → L in AAC72734. 1 Publication
Sequence conflicti613 – 6131P → L in AAF21782. 1 Publication
Sequence conflicti613 – 6131P → L in AAI20588. 1 Publication
Sequence conflicti651 – 6511R → H in AAC72734. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060194 mRNA. Translation: AAC72734.1.
AH008971 Genomic DNA. Translation: AAF21782.1.
AF416641 mRNA. Translation: AAL39015.1.
AC148976 Genomic DNA. No translation available.
BC120587 mRNA. Translation: AAI20588.1.
AK144472 mRNA. Translation: BAE25907.1.
CCDSiCCDS20860.1. [Q0VBL6-1]
RefSeqiNP_058564.2. NM_016868.3. [Q0VBL6-1]
UniGeneiMm.135110.

Genome annotation databases

EnsembliENSMUST00000037762; ENSMUSP00000048248; ENSMUSG00000004328. [Q0VBL6-1]
GeneIDi53417.
KEGGimmu:53417.
UCSCiuc009fir.2. mouse. [Q0VBL6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF060194 mRNA. Translation: AAC72734.1 .
AH008971 Genomic DNA. Translation: AAF21782.1 .
AF416641 mRNA. Translation: AAL39015.1 .
AC148976 Genomic DNA. No translation available.
BC120587 mRNA. Translation: AAI20588.1 .
AK144472 mRNA. Translation: BAE25907.1 .
CCDSi CCDS20860.1. [Q0VBL6-1 ]
RefSeqi NP_058564.2. NM_016868.3. [Q0VBL6-1 ]
UniGenei Mm.135110.

3D structure databases

ProteinModelPortali Q0VBL6.
SMRi Q0VBL6. Positions 10-340.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q0VBL6.

Proteomic databases

PRIDEi Q0VBL6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000037762 ; ENSMUSP00000048248 ; ENSMUSG00000004328 . [Q0VBL6-1 ]
GeneIDi 53417.
KEGGi mmu:53417.
UCSCi uc009fir.2. mouse. [Q0VBL6-1 ]

Organism-specific databases

CTDi 64344.
MGIi MGI:1859778. Hif3a.

Phylogenomic databases

eggNOGi NOG288942.
GeneTreei ENSGT00730000110711.
HOGENOMi HOG000234306.
HOVERGENi HBG060456.
KOi K09096.

Enzyme and pathway databases

Reactomei REACT_199107. Regulation of gene expression by Hypoxia-inducible Factor.
REACT_199108. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.

Miscellaneous databases

NextBioi 310241.
PROi Q0VBL6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q0VBL6.
Bgeei Q0VBL6.
CleanExi MM_HIF3A.
Genevestigatori Q0VBL6.

Family and domain databases

InterProi IPR011598. bHLH_dom.
IPR021537. HIF_alpha_subunit.
IPR001610. PAC.
IPR000014. PAS.
IPR013767. PAS_fold.
[Graphical view ]
Pfami PF11413. HIF-1. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
SSF55785. SSF55785. 2 hits.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
PS50112. PAS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3alpha."
    Gu Y.Z., Moran S.M., Hogenesch J.B., Wartman L., Bradfield C.A.
    Gene Expr. 7:205-213(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH ARNT.
  2. "Inhibitory PAS domain protein is a negative regulator of hypoxia-inducible gene expression."
    Makino Y., Cao R., Svensson K., Bertilsson G., Asman M., Tanaka H., Cao Y., Berkenstam A., Poellinger L.
    Nature 414:550-554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, INTERACTION WITH HIF1A.
    Strain: C57BL/6J.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 193-662 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiHIF3A_MOUSE
AccessioniPrimary (citable) accession number: Q0VBL6
Secondary accession number(s): E9QLB1
, Q3UN40, Q8VHR1, Q9QX54, Q9Z2I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi