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Q0VA64 (UBP16_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 16

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16
Gene names
Name:usp16
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length864 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B By similarity. HAMAP-Rule MF_03062

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). HAMAP-Rule MF_03062

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_03062

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03062.

Domain

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity. HAMAP-Rule MF_03062

Sequence similarities

Belongs to the peptidase C19 family. USP16 subfamily.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
Transcription
Transcription regulation
Ubl conjugation pathway
   Cellular componentNucleus
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Molecular functionActivator
Chromatin regulator
Hydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhistone deubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

mitosis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

protein homotetramerization

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncysteine-type endopeptidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

histone binding

Inferred from sequence or structural similarity. Source: UniProtKB

transcription coactivator activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin binding

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin thiolesterase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin-specific protease activity

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 864864Ubiquitin carboxyl-terminal hydrolase 16 HAMAP-Rule MF_03062
PRO_0000367507

Regions

Domain196 – 863668USP
Zinc finger43 – 12280UBP-type HAMAP-Rule MF_03062

Sites

Active site2051Nucleophile By similarity
Active site7981Proton acceptor By similarity
Metal binding211Zinc 1 By similarity
Metal binding231Zinc 1 By similarity
Metal binding451Zinc 2 By similarity
Metal binding481Zinc 2 By similarity
Metal binding711Zinc 3 By similarity
Metal binding741Zinc 3 By similarity
Metal binding791Zinc 2 By similarity
Metal binding871Zinc 2 By similarity
Metal binding911Zinc 3 By similarity
Metal binding1001Zinc 3 By similarity
Metal binding1131Zinc 1 By similarity
Metal binding1161Zinc 1 By similarity

Experimental info

Sequence conflict1061V → M in AAI21231. Ref.2
Sequence conflict4051A → V in AAI21231. Ref.2
Sequence conflict4521L → Q in AAI21231. Ref.2
Sequence conflict4951I → T in AAI21231. Ref.2
Sequence conflict5151T → A in AAI21231. Ref.2
Sequence conflict5291L → I in AAI21231. Ref.2
Sequence conflict5481R → G in AAI21231. Ref.2
Sequence conflict5721V → G in AAI21231. Ref.2
Sequence conflict7141T → K in AAI21231. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q0VA64 [UniParc].

Last modified December 11, 2013. Version 2.
Checksum: 8600F3A72FCAF9C7

FASTA86496,666
        10         20         30         40         50         60 
MVKKRGKNLP AQDSLDAEPV CKHLRKALDE GSVKKALVNV EWTVCQECQA DNKEKNNSDD 

        70         80         90        100        110        120 
ELVEDPSVWL CLKCGHRGCG RNSASQHALN HYNTPRSEPH CLVLSVDMWS AWCYLCDNEV 

       130        140        150        160        170        180 
PYNRSSRLGQ LVDYLQRKAK AKSKSTDSAA LDEEVKAEIV AENEVKIEDQ EEKPKGQAKW 

       190        200        210        220        230        240 
DKASSTQNNS TEPTVKGLSN LGNTCFFNAV MQNLSQTPAV RELLNEAKTL KKPVTVPLPD 

       250        260        270        280        290        300 
SSSPTNVEVH LEQQPGPLTL AMWQFLTEMQ ETKKGVVTPK EVFSQVCKKA IRFKGYQQQD 

       310        320        330        340        350        360 
SQELLRYLLD GMRGEEIQRV SLAMSKSLQN TLDEEEIKKI VKDSEKRRTI PNFVDHLFGG 

       370        380        390        400        410        420 
ELTSTIMCEE CHTVSLVHEP FLDLSLPVLD DVIVKKNSQK SSAPAPERKE EEENDDGYIK 

       430        440        450        460        470        480 
ERDEASPGAS KHLQKKAKKA AKKQAKNQRR QLKMQGKTVL LTDVAKQECS EDEEEIAPNN 

       490        500        510        520        530        540 
TESEANTRPD DEVPIADGLN TMKSDLSALE NGSETIESAM ERVTEDTDLD TSGHNTESVE 

       550        560        570        580        590        600 
MNAMELVRNM ENNNNNNTDV NKTLERTEGS GVDSMEATAA VDNGNADTVC VDDTEAANGL 

       610        620        630        640        650        660 
LDCSAASMDN ELTNSLNRLK LSSDIEPTQV EIEILPDQQQ PHTQIYEVIN EDPKTAFSTL 

       670        680        690        700        710        720 
SERKDLPLDG YSVLSCLYQF THKETLTGNN KLLCNVCTRK QASRLNNSNK GEKTFVYTNA 

       730        740        750        760        770        780 
KKQMLVSDPS PILTLHLKRF QQNGFNLRKI NRHIKFPEVL DLAPFCTSKC KNIPAGESRL 

       790        800        810        820        830        840 
LYSLYGVIEH SGSMRSGHYT AFVKLRRPNQ QLCEMVLKGV IPEVSGSEPG QGSWYHISDS 

       850        860 
HVQAVSLSRV LSSQAYLLFY ERML 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAMC01089338 Genomic DNA. No translation available.
AAMC01089339 Genomic DNA. No translation available.
BC121230 mRNA. Translation: AAI21231.1.
RefSeqNP_001072158.1. NM_001078690.1.
UniGeneStr.20151.

3D structure databases

ProteinModelPortalQ0VA64.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSXETT00000043701; ENSXETP00000043701; ENSXETG00000020255.
GeneID447956.
KEGGxtr:447956.

Organism-specific databases

CTD10600.
XenbaseXB-GENE-1007164. usp16.

Phylogenomic databases

eggNOGCOG5560.
GeneTreeENSGT00750000117419.
HOGENOMHOG000154755.
HOVERGENHBG062704.
KOK11844.
TreeFamTF326075.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
HAMAPMF_03062. UBP16.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP16_XENTR
AccessionPrimary (citable) accession number: Q0VA64
Secondary accession number(s): F7BZB4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 11, 2013
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries