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Q0V9E9 (SETD8_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-lysine methyltransferase SETD8

EC=2.1.1.-
Alternative name(s):
Histone-lysine N-methyltransferase SETD8
EC=2.1.1.43
SET domain-containing protein 8
Gene names
Name:setd8
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration By similarity.

Catalytic activity

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Subcellular location

Nucleus By similarity. Chromosome By similarity. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.

Contains 1 SET domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336N-lysine methyltransferase SETD8
PRO_0000317002

Regions

Domain200 – 321122SET
Region210 – 2123S-adenosyl-L-methionine binding By similarity
Region282 – 2832S-adenosyl-L-methionine binding By similarity

Sites

Binding site2551S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0V9E9 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 422D7B38C168DCA1

FASTA33638,237
        10         20         30         40         50         60 
MGRGKKMSKP GDGRSGDVPD TGRTGGTNEN HPKINGEVAH LGQPKIYSFM SPTKSPSARP 

        70         80         90        100        110        120 
PLQEENSVAH HESKCPGKPL TETRKKAEVE KKRISSGTEL SVKPSEQRET ECNSIGEFLD 

       130        140        150        160        170        180 
PKQEQTDVRR NIALPPADKL NHQKMVKDKP LRRKAQRKKS PNRKLTDYYP VRRSCRKSKT 

       190        200        210        220        230        240 
ELESEEKKRI DELIQTGKEE GMKMDMITGK GRGVIATRDF QRGEFVVEYH GDLIEITDAK 

       250        260        270        280        290        300 
RREATYAQDS NTGCYMYYFQ YLNKTYCIDA TRETGRLGRL INHSKSGNCH TKLHNINNVP 

       310        320        330 
HLILVASRDI NVGEELLYDY GDRRKSSLEA HPWLKN 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: N6.
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC121601 mRNA. Translation: AAI21602.1.
RefSeqNP_001072815.1. NM_001079347.1.
UniGeneStr.11037.

3D structure databases

ProteinModelPortalQ0V9E9.
SMRQ0V9E9. Positions 176-336.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000012803.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSXETT00000012803; ENSXETP00000012803; ENSXETG00000005820.
GeneID780276.
KEGGxtr:780276.

Organism-specific databases

CTD387893.
XenbaseXB-GENE-489177. setd8.

Phylogenomic databases

eggNOGCOG2940.
GeneTreeENSGT00410000025501.
HOGENOMHOG000020818.
HOVERGENHBG067546.
KOK11428.
OMAGAKENEP.
OrthoDBEOG70KGRN.
TreeFamTF335181.

Gene expression databases

BgeeQ0V9E9.

Family and domain databases

InterProIPR016858. Hist_H4-K20_MeTrfase.
IPR001214. SET_dom.
[Graphical view]
PfamPF00856. SET. 1 hit.
[Graphical view]
PIRSFPIRSF027717. Histone_H4-K20_mtfrase. 1 hit.
SMARTSM00317. SET. 1 hit.
[Graphical view]
PROSITEPS51571. SAM_MT43_PR_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSETD8_XENTR
AccessionPrimary (citable) accession number: Q0V9E9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 5, 2006
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families