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Q0V9E9

- SETD8_XENTR

UniProt

Q0V9E9 - SETD8_XENTR

Protein

N-lysine methyltransferase SETD8

Gene

setd8

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei255 – 2551S-adenosyl-L-methioninePROSITE-ProRule annotation

    GO - Molecular functioni

    1. histone-lysine N-methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. histone lysine methylation Source: GOC
    2. mitotic nuclear division Source: UniProtKB-KW
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Repressor, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-lysine methyltransferase SETD8 (EC:2.1.1.-)
    Alternative name(s):
    Histone-lysine N-methyltransferase SETD8 (EC:2.1.1.43)
    SET domain-containing protein 8
    Gene namesi
    Name:setd8
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-489177. setd8.

    Subcellular locationi

    Nucleus By similarity. Chromosome By similarity
    Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms By similarity.By similarity

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336N-lysine methyltransferase SETD8PRO_0000317002Add
    BLAST

    Expressioni

    Gene expression databases

    BgeeiQ0V9E9.

    Interactioni

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000012803.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0V9E9.
    SMRiQ0V9E9. Positions 176-336.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini200 – 321122SETPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni210 – 2123S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni282 – 2832S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.PROSITE-ProRule annotation
    Contains 1 SET domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2940.
    GeneTreeiENSGT00410000025501.
    HOGENOMiHOG000020818.
    HOVERGENiHBG067546.
    KOiK11428.
    OMAiGAKENEP.
    OrthoDBiEOG70KGRN.
    TreeFamiTF335181.

    Family and domain databases

    InterProiIPR016858. Hist_H4-K20_MeTrfase.
    IPR001214. SET_dom.
    [Graphical view]
    PfamiPF00856. SET. 1 hit.
    [Graphical view]
    PIRSFiPIRSF027717. Histone_H4-K20_mtfrase. 1 hit.
    SMARTiSM00317. SET. 1 hit.
    [Graphical view]
    PROSITEiPS51571. SAM_MT43_PR_SET. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0V9E9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRGKKMSKP GDGRSGDVPD TGRTGGTNEN HPKINGEVAH LGQPKIYSFM    50
    SPTKSPSARP PLQEENSVAH HESKCPGKPL TETRKKAEVE KKRISSGTEL 100
    SVKPSEQRET ECNSIGEFLD PKQEQTDVRR NIALPPADKL NHQKMVKDKP 150
    LRRKAQRKKS PNRKLTDYYP VRRSCRKSKT ELESEEKKRI DELIQTGKEE 200
    GMKMDMITGK GRGVIATRDF QRGEFVVEYH GDLIEITDAK RREATYAQDS 250
    NTGCYMYYFQ YLNKTYCIDA TRETGRLGRL INHSKSGNCH TKLHNINNVP 300
    HLILVASRDI NVGEELLYDY GDRRKSSLEA HPWLKN 336
    Length:336
    Mass (Da):38,237
    Last modified:September 5, 2006 - v1
    Checksum:i422D7B38C168DCA1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC121601 mRNA. Translation: AAI21602.1.
    RefSeqiNP_001072815.1. NM_001079347.1.
    UniGeneiStr.11037.

    Genome annotation databases

    EnsembliENSXETT00000012803; ENSXETP00000012803; ENSXETG00000005820.
    GeneIDi780276.
    KEGGixtr:780276.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC121601 mRNA. Translation: AAI21602.1 .
    RefSeqi NP_001072815.1. NM_001079347.1.
    UniGenei Str.11037.

    3D structure databases

    ProteinModelPortali Q0V9E9.
    SMRi Q0V9E9. Positions 176-336.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000012803.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSXETT00000012803 ; ENSXETP00000012803 ; ENSXETG00000005820 .
    GeneIDi 780276.
    KEGGi xtr:780276.

    Organism-specific databases

    CTDi 387893.
    Xenbasei XB-GENE-489177. setd8.

    Phylogenomic databases

    eggNOGi COG2940.
    GeneTreei ENSGT00410000025501.
    HOGENOMi HOG000020818.
    HOVERGENi HBG067546.
    KOi K11428.
    OMAi GAKENEP.
    OrthoDBi EOG70KGRN.
    TreeFami TF335181.

    Gene expression databases

    Bgeei Q0V9E9.

    Family and domain databases

    InterProi IPR016858. Hist_H4-K20_MeTrfase.
    IPR001214. SET_dom.
    [Graphical view ]
    Pfami PF00856. SET. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF027717. Histone_H4-K20_mtfrase. 1 hit.
    SMARTi SM00317. SET. 1 hit.
    [Graphical view ]
    PROSITEi PS51571. SAM_MT43_PR_SET. 1 hit.
    PS50280. SET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: N6.
      Tissue: Ovary.

    Entry informationi

    Entry nameiSETD8_XENTR
    AccessioniPrimary (citable) accession number: Q0V9E9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3