Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hepatocyte growth factor-regulated tyrosine kinase substrate

Gene

HGS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri160 – 22061FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-182971. EGFR downregulation.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte growth factor-regulated tyrosine kinase substrate
Gene namesi
Name:HGS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 777777Hepatocyte growth factor-regulated tyrosine kinase substratePRO_0000274196Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei207 – 2071N6-acetyllysineBy similarity
Modified residuei308 – 3081PhosphotyrosineBy similarity
Modified residuei329 – 3291PhosphotyrosineBy similarity
Modified residuei334 – 3341PhosphotyrosineBy similarity
Modified residuei549 – 5491N6-succinyllysineBy similarity

Post-translational modificationi

Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ0V8S0.
PRIDEiQ0V8S0.

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Part of a complex at least composed of HSG, STAM2 (or probably STAM) and EPS15. Interacts with STAM. Interacts with STAM2. Interacts with EPS15; the interaction is direct, calcium-dependent and inhibited by SNAP25. Interacts with NF2; the interaction is direct. Interacts with ubiquitin; the interaction is direct. Interacts with VPS37C. Interacts with SMAD1, SMAD2 and SMAD3. Interacts with TSG101. Interacts with SNAP25; the interaction is direct and decreases with addition of increasing concentrations of free calcium. Interacts with SNX1; the interaction is direct. Component of a 550 kDa membrane complex at least composed of HGS and SNX1 but excluding EGFR. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with TRAK2. Interacts (via UIM domain) with UBQLN1 (via ubiquitin-like domain) (By similarity). Interacts with ARRDC3. Identified in a complex containing at least ARRDC4, V2R and HGS (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000527.

Structurei

3D structure databases

ProteinModelPortaliQ0V8S0.
SMRiQ0V8S0. Positions 6-221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 143129VHSPROSITE-ProRule annotationAdd
BLAST
Domaini258 – 27720UIMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 541317Interaction with SNX1By similarityAdd
BLAST
Regioni443 – 54199Interaction with SNAP25 and TRAK2By similarityAdd
BLAST
Regioni452 – 570119Interaction with STAMBy similarityAdd
BLAST
Regioni478 – 777300Interaction with NF2By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi503 – 772270Gln-richAdd
BLAST

Domaini

Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.By similarity
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions (By similarity).By similarity

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri160 – 22061FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1818. Eukaryota.
ENOG410XNRF. LUCA.
GeneTreeiENSGT00670000098022.
HOGENOMiHOG000044175.
HOVERGENiHBG062917.
InParanoidiQ0V8S0.
KOiK12182.
OMAiKRMWYEQ.
OrthoDBiEOG7N37CN.
TreeFamiTF314470.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR024641. HRS_helical.
IPR017073. Ubi-bd_Hrs_VPS27.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF12210. Hrs_helical. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PIRSFiPIRSF036956. Hrs_Vps27. 1 hit.
SMARTiSM00064. FYVE. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0V8S0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRGSGTFER LLDKATSQLL LETDWESILQ ICDLIRQGDT QAKYAVSSIK
60 70 80 90 100
KKVNDKNPHV ALYALEVMES VVKNCGQTVH DEVANKQTME ELKDLLKRQV
110 120 130 140 150
EVNVRNKILY LIQAWAHAFR NEPKYKVVQD TYQIMKVEGH VFPEFKESDA
160 170 180 190 200
MFAAERAPDW VDAEECHRCR VQFGVMTRKH HCRACGQIFC GKCSSKYSTI
210 220 230 240 250
PKFGIEKEVR VCEPCFEQLN KKAEGKAAST TELPPEYLTS PLSQQSQLPP
260 270 280 290 300
KRDETALQEE EELQLALALS QSEAEEKERM RQKSAYTAYP KAEPTPVASS
310 320 330 340 350
APPASSLYSS PVNSSAPLAE DIDPELARYL NRNYWEKKQE EARKSPTPSA
360 370 380 390 400
PVPLTEPTAQ PGEGHAIPAN VETSLPETDP QAVTAAGAAF SEQYQNGESE
410 420 430 440 450
ESHAQFLKAL QNAVTTFVNR MKSNHVRGRS ITNDSAVLSL FQSINTMHPQ
460 470 480 490 500
LLELLNQLDE RRLYYEGLQD KLAQIRDARG ALSALREEHR EKLRRAAEEA
510 520 530 540 550
ERQRQIQLAQ KLEIMRQKKQ EYLEVQRQLA IQRLQEQEKE RQMRLEQQKQ
560 570 580 590 600
TIQMRAQMPA FSLPYAQLQA MPAAGGVLYQ PSGPASFAGT FSPAGSVEGS
610 620 630 640 650
PMHTMYMSQP APAASGPYPS MPAAAADPSM VSAYMYPAGA AGAQAAAQGP
660 670 680 690 700
AGPTTSPAYS SYQPTPTQGY QTVASQAPQS LPAISQPPQS GTMGYMGSQS
710 720 730 740 750
VSMGYQPYSM QNLMPTLPGQ DAPLPPPQQP YISGQQPVYQ QMAPSSGPPQ
760 770
QQPPVAQQPP AQGPPAQGSE AQLISFD
Length:777
Mass (Da):85,786
Last modified:September 5, 2006 - v1
Checksum:i13DD0CE832AD56B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti321 – 3211D → E in AAI11314 (Ref. 2) Curated
Sequence conflicti392 – 3921Missing in AAI11314 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026148 mRNA. Translation: ABG66987.1.
BC111313 mRNA. Translation: AAI11314.1.
RefSeqiNP_001039554.1. NM_001046089.1.
XP_005221090.1. XM_005221033.3.
UniGeneiBt.6724.

Genome annotation databases

EnsembliENSBTAT00000000527; ENSBTAP00000000527; ENSBTAG00000000411.
GeneIDi511582.
KEGGibta:511582.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026148 mRNA. Translation: ABG66987.1.
BC111313 mRNA. Translation: AAI11314.1.
RefSeqiNP_001039554.1. NM_001046089.1.
XP_005221090.1. XM_005221033.3.
UniGeneiBt.6724.

3D structure databases

ProteinModelPortaliQ0V8S0.
SMRiQ0V8S0. Positions 6-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000000527.

Proteomic databases

PaxDbiQ0V8S0.
PRIDEiQ0V8S0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000000527; ENSBTAP00000000527; ENSBTAG00000000411.
GeneIDi511582.
KEGGibta:511582.

Organism-specific databases

CTDi9146.

Phylogenomic databases

eggNOGiKOG1818. Eukaryota.
ENOG410XNRF. LUCA.
GeneTreeiENSGT00670000098022.
HOGENOMiHOG000044175.
HOVERGENiHBG062917.
InParanoidiQ0V8S0.
KOiK12182.
OMAiKRMWYEQ.
OrthoDBiEOG7N37CN.
TreeFamiTF314470.

Enzyme and pathway databases

ReactomeiR-BTA-182971. EGFR downregulation.
R-BTA-432720. Lysosome Vesicle Biogenesis.
R-BTA-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR024641. HRS_helical.
IPR017073. Ubi-bd_Hrs_VPS27.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF12210. Hrs_helical. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PIRSFiPIRSF036956. Hrs_Vps27. 1 hit.
SMARTiSM00064. FYVE. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiHGS_BOVIN
AccessioniPrimary (citable) accession number: Q0V8S0
Secondary accession number(s): Q2NKZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: September 5, 2006
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.