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Protein

5-methylcytosine rRNA methyltransferase NSUN4

Gene

NSUN4

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in mitochondrial ribosome assembly. 5-methylcytosine rRNA methyltransferase that probably is involved in mitochondrial ribosome small subunit (SSU) maturation by methylation of mitochondrial 12S rRNA; the function is independent of MTERFD2/MTERF4 and assembled mitochondrial ribosome large subunit (LSU). Targeted to LSU by MTERFD2/MTERF4 and probably is involved in a final step in ribosome biogenesis to ensure that SSU and LSU are assembled. In vitro can methylate 16S rRNA of the LSU; the methylation is enhanced by MTERFD/MTERF4 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei237S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei255S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei310NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, rRNA-binding, Transferase
Biological processRibosome biogenesis, rRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
5-methylcytosine rRNA methyltransferase NSUN4 (EC:2.1.1.-)
Alternative name(s):
NOL1/NOP2/Sun domain family member 4
Gene namesi
Name:NSUN4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000289233? – 3845-methylcytosine rRNA methyltransferase NSUN4
Transit peptidei1 – ?MitochondrionBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei206PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ0V8R7.
PRIDEiQ0V8R7.

Expressioni

Gene expression databases

BgeeiENSBTAG00000015891.

Interactioni

Subunit structurei

Heterodimer with MTERF4/MTERFD2; this interaction may be required for NSUN4 recruitment to the mitochondrial large ribosomal subunit.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021120.

Structurei

3D structure databases

ProteinModelPortaliQ0V8R7.
SMRiQ0V8R7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 187S-adenosyl-L-methionine bindingPROSITE-ProRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2198. Eukaryota.
COG0144. LUCA.
GeneTreeiENSGT00660000095341.
HOGENOMiHOG000017426.
HOVERGENiHBG106892.
InParanoidiQ0V8R7.
KOiK21970.
OMAiEPKFPAT.
OrthoDBiEOG091G07PM.
TreeFamiTF321304.

Family and domain databases

InterProiView protein in InterPro
IPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF01189. Methyltr_RsmB-F. 1 hit.
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51686. SAM_MT_RSMB_NOP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0V8R7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALVVRGVR DMLKRADFAT VPRRQRHKKK WASTEPKFPA TRLALQNFDM
60 70 80 90 100
TYSVQFGDLW PSIRVSLLSE QKYGALVNNF AAGDRVSAEL EQLKARDFVN
110 120 130 140 150
EAVFHREPEP ENSQTAAPSP ASWACSPNLR CFTFTRGDVS RFPPARLGSL
160 170 180 190 200
GLMDYYLMDA ASLLPVLALG LQPGDTVLDL CAAPGGKTLA LLQTGCCRNL
210 220 230 240 250
AANDLSTSRT SRLQRVLHSY VPQDVRDKNR VRVTSWDGRK WGELEGDTYD
260 270 280 290 300
RVLVDVPCTT DRHSLHEEEN SIFQRSRKKE RQMLPVLQVQ LLAAGLLATK
310 320 330 340 350
PGGHIVYSTC SLSHLQNEYV VQGAIELLDN QYSIKVQVED LTHFRKLFMN
360 370 380
TFSFFPSCQV GELVIPNLMA NFGPMYFCKM CRMT
Length:384
Mass (Da):43,199
Last modified:May 29, 2007 - v2
Checksum:iC457DAD9CDED2BD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 13ML → IP in DY454541 (PubMed:16305752).Curated2
Sequence conflicti43L → P in DY454541 (PubMed:16305752).Curated1
Sequence conflicti167L → P in DY454541 (PubMed:16305752).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026151 mRNA. Translation: ABG66990.1.
DY454541 mRNA. No translation available.
RefSeqiNP_001178310.1. NM_001191381.1.
UniGeneiBt.36843.

Genome annotation databases

EnsembliENSBTAT00000021120; ENSBTAP00000021120; ENSBTAG00000015891.
GeneIDi618779.
KEGGibta:618779.

Similar proteinsi

Entry informationi

Entry nameiNSUN4_BOVIN
AccessioniPrimary (citable) accession number: Q0V8R7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: October 25, 2017
This is version 64 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome