Reviewed, UniProtKB/Swiss-Prot Q0V8L6 (PLD2_BOVIN)

Names and origin

Protein names

Recommended name:
    Phospholipase D2
      Short name=PLD 2
    EC=3.1.4.4
Alternative name(s):
    Choline phosphatase 2
    Phosphatidylcholine-hydrolyzing phospholipase D2

Gene names

Name:

PLD2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	933 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May have a role in signal-induced cytoskeletal regulation and/or endocytosis By similarity.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Enzyme regulation	Stimulated by phosphatidylinositol 4,5-bisphosphate and activated by the ADP-ribosylation factor-1 (ARF-1) By similarity.
Subunit structure	Interacts with EGFR and PIP5K1A By similarity.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the phospholipase D family. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Membrane
Domain	Repeat
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phosphoinositide binding Inferred from electronic annotation. Source: InterPro phospholipase D activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 933	933	Phospholipase D2		PRO_0000253038
Regions
	Domain	65 – 195	131	PX
	Domain	203 – 311	109	PH
	Domain	437 – 464	28	PLD phosphodiesterase 1
	Domain	751 – 778	28	PLD phosphodiesterase 2
	Region	441 – 788	348	Catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q0V8L6-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 8B5C417338397455	FASTA	933	105,766
	10 20 30 40 50 60 MAATPQSLFP SGDDLDSSQL QMEPDEVDTL KEGEDPADRM HPFLAIYHLQ PLKLHPLVFA 70 80 90 100 110 120 PGVPVIAQVV GTERYTSGSK VGTCTLYSVR LTHGDFTWTT KKKFRHFQEL HRDLLRHKVF 130 140 150 160 170 180 MSLLPLARFA VASSPAPEGD SREIPSLPRA GPEGSSRRTA SKQKYLENYL NRLLTMSFYR 190 200 210 220 230 240 NYHAMTEFLE VSQLSFIPDL GCKGLEGVIR KRSGGHRVPG LTCCGRDQVC YRWSKRWLVV 250 260 270 280 290 300 KDSFLLYMCL ETGAISFVQL FDPGFKVQVG KRSTEARYGV RVDTSHRSLI LKCSSYRQAR 310 320 330 340 350 360 WWAQEITELA QGPGRDFIQL HRHDSYAPPR PGTLARWFVN GAGYFAAVAD AILRAQEEIF 370 380 390 400 410 420 ITDWWLSPEI YLKRPAHSDD WRLDIMLKKK AEEGVHVSVL LFKEVELALA INSGYSKKAL 430 440 450 460 470 480 MLLHPNIKVM RHPDQVTLWA HHEKLLVVDQ VVAFLGGLDL AYGRWDDLHY RLTDLGDSSE 490 500 510 520 530 540 SAAPQPPTSC SDLPATPDLT HNQLFWLGKD YSNLITKDWV QLDRPFDDFI DRETMPRMPW 550 560 570 580 590 600 RDIGVVVHGS PARDLARHFI QRWNFTKTTK TKYKIPIYPY LLPKSTSTAN QLPFTLSGGQ 610 620 630 640 650 660 CATVQVLRSV DRWSAGTLEN SILNAYLHTI RESQHFLYIE NQFFISCSDG RTVLNKVGDE 670 680 690 700 710 720 IVDRILKAHK QGQCFRVYVL LPLLPGFEGD ISTGGGNSIQ AILHFTYRTL CRGEYSILHR 730 740 750 760 770 780 LKAAMGTEWR NYISVCGLRT HGELGGHPVS ELIYIHSKML IADDRTVIIG SANINDRSLL 790 800 810 820 830 840 GKRDSELAVL IEDTEMEPSL MNGVEYQAGR FALSLRKHCF SVILGAAARP HLDLRDPVCD 850 860 870 880 890 900 AFFQLWQDTA ESNANIYEQI FRCLPSNATR SLRALREYVV VEPLATVSPP LARSELNQVQ 910 920 930 GHLVHFPLKF LEDEYLLPSL GSKEGVMPLE VWT

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pons.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT026202 mRNA. Translation: ABG67041.1. BC123547 mRNA. Translation: AAI23548.1.
IPI	IPI00704116.
RefSeq	NP_001069295.1.
UniGene	Bt.1009
3D structure databases
SMR	Q0V8L6. Positions 62-193.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0V8L6.
Genome annotation databases
GeneID	522159.
KEGG	bta:522159.
Organism-specific databases
CTD	522159.
Phylogenomic databases
eggNOG	maNOG13101.
HOVERGEN	Q0V8L6.
Enzyme and pathway databases
BRENDA	3.1.4.4. 251.
Family and domain databases
InterPro	IPR015679. Phospholipase_D. IPR001849. Pleckstrin_homology. IPR001736. PLipase_D/transphosphatidylase. IPR016555. PLipase_D_euk. IPR001683. PX. [Graphical view]
Gene3D	G3DSA:3.30.1520.10. PX. 1 hit.
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00614. PLDc. 2 hits. PF00787. PX. 1 hit. [Graphical view]
PIRSF	PIRSF009376. Phospholipase_D_euk. 1 hit.
SMART	SM00233. PH. 1 hit. SM00155. PLDc. 2 hits. SM00312. PX. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. PS50035. PLD. 2 hits. PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLD2_BOVIN

Accession

Primary (citable) accession number: Q0V8L6
Secondary accession number(s): Q08DV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	September 5, 2006
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)