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Q0V8B6 (TPP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tripeptidyl-peptidase 1
Short name=TPP-1
EC=3.4.14.9
Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
Short name=TPP-I
Gene names
Name:TPP1
Synonyms:CLN2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus By similarity.

Catalytic activity

Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Lysosome By similarity. Melanosome By similarity.

Post-translational modification

Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity By similarity.

Sequence similarities

Belongs to the peptidase S53 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Propeptide20 – 195176Removed in mature form By similarity
PRO_0000291586
Chain196 – 563368Tripeptidyl-peptidase 1
PRO_0000291587

Sites

Active site2721Charge relay system By similarity
Active site2761Charge relay system By similarity
Active site4751Charge relay system By similarity
Metal binding5171Calcium By similarity
Metal binding5181Calcium; via carbonyl oxygen By similarity
Metal binding5391Calcium; via carbonyl oxygen By similarity
Metal binding5411Calcium; via carbonyl oxygen By similarity
Metal binding5431Calcium By similarity

Amino acid modifications

Glycosylation2101N-linked (GlcNAc...) Potential
Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation3131N-linked (GlcNAc...) Potential
Glycosylation4431N-linked (GlcNAc...) Potential
Disulfide bond111 ↔ 122 By similarity
Disulfide bond365 ↔ 526 By similarity
Disulfide bond522 ↔ 537 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0V8B6 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 6D23396D375612C1

FASTA56361,352
        10         20         30         40         50         60 
MGPRSGLLGL FALFVAGKCS YSPEPDQQRR LPPGWVSLGR ADPEEELSLT FALRQQNVKR 

        70         80         90        100        110        120 
LSELVQAVSD PGSPRYGKYL TLEDVAELVR PSPLTLHTVQ KWLLAAGARN CHSVTTQDFL 

       130        140        150        160        170        180 
TCWLSVRQAE LLLSGAEFHH YVGGPAETHA VRSLHPYRLP KALAPHVDFV GGLHRFPPTS 

       190        200        210        220        230        240 
TLRQHPEPQV PGTVGLHLGV TPSVIRKRYN LTAQDVGSGT TNNSQACAQF LEQYFHDSDL 

       250        260        270        280        290        300 
AEFMRLFGGD FAHQASVARV VGQQGRGRAG IEASLDVEYL MSAGANISTW VYSSPGRHES 

       310        320        330        340        350        360 
QEPFLQWLLL LSNESALPYV HTVSYGDDED SLSSTYIQRV NTELMKAAAR GLTLLFASGD 

       370        380        390        400        410        420 
SGAGCWSVSG RHQFRPSFPA SSPYVTTVGG TSFQNPFRVT DEVVDYISGG GFSNVFPRPS 

       430        440        450        460        470        480 
YQEEAVTRYL SSSPHLPPSS YFNASGRAYP DVAALSDGYW VVSNHVPIPW VSGTSASTPV 

       490        500        510        520        530        540 
FGGLLSLINE HRILRGLPPL GFLNPRLYQK HGAGLFDVTR GCHESCLNEE VEGQGFCSGP 

       550        560 
GWDPVTGWGT PNFPALLKTL MNP

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Heart ventricle.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BT026303 mRNA. Translation: ABG81459.1.
BC119827 mRNA. Translation: AAI19828.1.
IPIIPI00721428.
RefSeqNP_001069186.1. NM_001075718.1.
UniGeneBt.35140.

3D structure databases

ProteinModelPortalQ0V8B6.
ModBaseSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000020469.

Proteomic databases

PaxDbQ0V8B6.
PRIDEQ0V8B6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID515575.
KEGGbta:515575.

Organism-specific databases

CTD1200.

Phylogenomic databases

eggNOGCOG4934.
HOGENOMHOG000171253.
HOVERGENHBG004449.
InParanoidQ0V8B6.
KOK01279.
OrthoDBEOG42Z4PZ.

Family and domain databases

Gene3D3.40.50.200. 1 hit.
InterProIPR015366. Peptidase_S53_propep.
IPR000209. Peptidase_S8/S53_dom.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamPF00082. Peptidase_S8. 1 hit.
PF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTSM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMSSF52743. Pept_S8_S53. 1 hit.
SSF54897. Prot_inh_propept. 1 hit.
ProtoNetSearch...

Other

NextBio20871898.

Entry information

Entry nameTPP1_BOVIN
AccessionPrimary (citable) accession number: Q0V8B6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: September 5, 2006
Last modified: April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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