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Protein

Tripeptidyl-peptidase 1

Gene

TPP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus (By similarity).By similarity

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei272Charge relay systemBy similarity1
Active sitei276Charge relay systemBy similarity1
Active sitei475Charge relay systemBy similarity1
Metal bindingi517CalciumBy similarity1
Metal bindingi518Calcium; via carbonyl oxygenBy similarity1
Metal bindingi539Calcium; via carbonyl oxygenBy similarity1
Metal bindingi541Calcium; via carbonyl oxygenBy similarity1
Metal bindingi543CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS53.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 1 (EC:3.4.14.9)
Short name:
TPP-1
Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
Short name:
TPP-I
Gene namesi
Name:TPP1
Synonyms:CLN2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Lysosome By similarity
  • Melanosome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
PropeptideiPRO_000029158620 – 195Removed in mature formBy similarityAdd BLAST176
ChainiPRO_0000291587196 – 563Tripeptidyl-peptidase 1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi111 ↔ 122By similarity
Glycosylationi210N-linked (GlcNAc...)Sequence analysis1
Glycosylationi222N-linked (GlcNAc...)Sequence analysis1
Glycosylationi286N-linked (GlcNAc...)Sequence analysis1
Glycosylationi313N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi365 ↔ 526By similarity
Glycosylationi443N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi522 ↔ 537By similarity

Post-translational modificationi

Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ0V8B6.
PeptideAtlasiQ0V8B6.
PRIDEiQ0V8B6.

Interactioni

Subunit structurei

Monomer. Interacts with CLN5.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020469.

Structurei

3D structure databases

ProteinModelPortaliQ0V8B6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 563Peptidase S53Add BLAST365

Sequence similaritiesi

Contains 1 peptidase S53 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IICY. Eukaryota.
COG4934. LUCA.
HOGENOMiHOG000171253.
HOVERGENiHBG004449.
InParanoidiQ0V8B6.
KOiK01279.

Family and domain databases

CDDicd04056. Peptidases_S53. 1 hit.
cd11377. Pro-peptidase_S53. 1 hit.
Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR009020. Propept_inh.
IPR015366. S53_propep.
IPR030400. Sedolisin_dom.
[Graphical view]
PfamiPF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTiSM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS51695. SEDOLISIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0V8B6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPRSGLLGL FALFVAGKCS YSPEPDQQRR LPPGWVSLGR ADPEEELSLT
60 70 80 90 100
FALRQQNVKR LSELVQAVSD PGSPRYGKYL TLEDVAELVR PSPLTLHTVQ
110 120 130 140 150
KWLLAAGARN CHSVTTQDFL TCWLSVRQAE LLLSGAEFHH YVGGPAETHA
160 170 180 190 200
VRSLHPYRLP KALAPHVDFV GGLHRFPPTS TLRQHPEPQV PGTVGLHLGV
210 220 230 240 250
TPSVIRKRYN LTAQDVGSGT TNNSQACAQF LEQYFHDSDL AEFMRLFGGD
260 270 280 290 300
FAHQASVARV VGQQGRGRAG IEASLDVEYL MSAGANISTW VYSSPGRHES
310 320 330 340 350
QEPFLQWLLL LSNESALPYV HTVSYGDDED SLSSTYIQRV NTELMKAAAR
360 370 380 390 400
GLTLLFASGD SGAGCWSVSG RHQFRPSFPA SSPYVTTVGG TSFQNPFRVT
410 420 430 440 450
DEVVDYISGG GFSNVFPRPS YQEEAVTRYL SSSPHLPPSS YFNASGRAYP
460 470 480 490 500
DVAALSDGYW VVSNHVPIPW VSGTSASTPV FGGLLSLINE HRILRGLPPL
510 520 530 540 550
GFLNPRLYQK HGAGLFDVTR GCHESCLNEE VEGQGFCSGP GWDPVTGWGT
560
PNFPALLKTL MNP
Length:563
Mass (Da):61,352
Last modified:September 5, 2006 - v1
Checksum:i6D23396D375612C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026303 mRNA. Translation: ABG81459.1.
BC119827 mRNA. Translation: AAI19828.1.
RefSeqiNP_001069186.1. NM_001075718.1.
UniGeneiBt.35140.

Genome annotation databases

GeneIDi515575.
KEGGibta:515575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026303 mRNA. Translation: ABG81459.1.
BC119827 mRNA. Translation: AAI19828.1.
RefSeqiNP_001069186.1. NM_001075718.1.
UniGeneiBt.35140.

3D structure databases

ProteinModelPortaliQ0V8B6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000020469.

Protein family/group databases

MEROPSiS53.003.

Proteomic databases

PaxDbiQ0V8B6.
PeptideAtlasiQ0V8B6.
PRIDEiQ0V8B6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi515575.
KEGGibta:515575.

Organism-specific databases

CTDi1200.

Phylogenomic databases

eggNOGiENOG410IICY. Eukaryota.
COG4934. LUCA.
HOGENOMiHOG000171253.
HOVERGENiHBG004449.
InParanoidiQ0V8B6.
KOiK01279.

Family and domain databases

CDDicd04056. Peptidases_S53. 1 hit.
cd11377. Pro-peptidase_S53. 1 hit.
Gene3Di3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR009020. Propept_inh.
IPR015366. S53_propep.
IPR030400. Sedolisin_dom.
[Graphical view]
PfamiPF09286. Pro-kuma_activ. 1 hit.
[Graphical view]
SMARTiSM00944. Pro-kuma_activ. 1 hit.
[Graphical view]
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS51695. SEDOLISIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPP1_BOVIN
AccessioniPrimary (citable) accession number: Q0V8B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: September 5, 2006
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.