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Protein

Serine/threonine-protein kinase H1

Gene

PSKH1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activity depends on Ca2+ concentration.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei127 – 1271ATPPROSITE-ProRule annotation
Active sitei218 – 2181Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1129ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase H1 (EC:2.7.11.1)
Alternative name(s):
Protein serine kinase H1
Short name:
PSK-H1
Gene namesi
Name:PSKH1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

  • Golgi apparatus By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Nucleus speckle By similarity
  • Endoplasmic reticulum membrane By similarity; Lipid-anchor By similarity
  • Cell membrane By similarity; Lipid-anchor By similarity
  • Cytoplasm By similarity

  • Note: Localized in the brefeldin A- sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm. Found to concentrate in splicing factor compartments (SFCs) within the nucleus of interphase cells. The acylation-negative form may be only cytoplasmic and nuclear. Acylation seems to allow the sequestering to the intracellular membranes. Myristoylation may mediate targeting to the intracellular non-Golgi membranes and palmitoylation may mediate the targeting to the Golgi membranes. Dual acylation is required to stabilize the interaction with Golgi membranes (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 424423Serine/threonine-protein kinase H1PRO_0000292952Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity
Modified residuei380 – 3801Phosphoserine; by autocatalysisSequence analysis
Modified residuei381 – 3811Phosphoserine; by autocatalysisSequence analysis

Post-translational modificationi

Autophosphorylated on serine residues.By similarity
Myristoylated. Required for membrane association. Prerequisite for palmitoylation to occur (By similarity).By similarity
Palmitoylated.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ0V7M1.
PRIDEiQ0V7M1.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024011.

Structurei

3D structure databases

ProteinModelPortaliQ0V7M1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini98 – 355258Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ0V7M1.
KOiK08808.
OMAiAASQCAN.
OrthoDBiEOG7WHH9K.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q0V7M1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCGTSKVLP EPPKDVQLDL VKKVEPFSGT KSDVYKHFIT EVDSVGPLKG
60 70 80 90 100
GFPAASQGAN PSPGTPRTSH TEPPSEPPRR ARVAKYRAKF DPRVTAKYDI
110 120 130 140 150
KALIGRGSFS RVVRVEHRAT RQPYAIKMIE TKYREGREVC ESELRVLRRV
160 170 180 190 200
RHANIIQLVE VFETQERVYM VMELATGGEL FDRIIAKGSF TERDATRVLQ
210 220 230 240 250
MVLDGVRYLH ALGITHRDLK PENLLYYHPG TDSKIIITDF GLASARKKGD
260 270 280 290 300
DCLMKTTCGT PEYIAPEVLV RKPYTNSVDM WALGVIAYIL LSGTMPFEDD
310 320 330 340 350
NRTRLYRQIL RGKYSYSGEP WPSVSNLAKD FIDRLLTVDP GARMTALQAL
360 370 380 390 400
RHPWVVSMAA SSSMKNLHRS ISQNLLKRAS SRCQSTKSAQ STRSSRSTRS
410 420
NKSRRVRERE LRELNLRYQQ QYNG
Length:424
Mass (Da):48,009
Last modified:September 5, 2006 - v1
Checksum:i83C6943A3BBDCD2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026549 mRNA. Translation: ABH06336.1.
RefSeqiNP_001068881.1. NM_001075413.1.
UniGeneiBt.2979.

Genome annotation databases

EnsembliENSBTAT00000024011; ENSBTAP00000024011; ENSBTAG00000018037.
GeneIDi509656.
KEGGibta:509656.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT026549 mRNA. Translation: ABH06336.1.
RefSeqiNP_001068881.1. NM_001075413.1.
UniGeneiBt.2979.

3D structure databases

ProteinModelPortaliQ0V7M1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024011.

Proteomic databases

PaxDbiQ0V7M1.
PRIDEiQ0V7M1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000024011; ENSBTAP00000024011; ENSBTAG00000018037.
GeneIDi509656.
KEGGibta:509656.

Organism-specific databases

CTDi5681.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ0V7M1.
KOiK08808.
OMAiAASQCAN.
OrthoDBiEOG7WHH9K.
TreeFamiTF314166.

Miscellaneous databases

NextBioi20869072.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiKPSH1_BOVIN
AccessioniPrimary (citable) accession number: Q0V7M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: September 5, 2006
Last modified: May 11, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.