Q0V6M5 (LCPS_PHANO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

1. Lycopene beta-cyclase
   EC=5.5.1.19
   Alternative name(s):
   Lycopene cyclase
2. Phytoene synthase
   EC=2.5.1.32

Gene names

ORF Names:

SNOG_00339

Organism

Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum) [Reference proteome]

Taxonomic identifier

321614 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › dothideomyceta › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Phaeosphaeriaceae › Phaeosphaeria › Phaeosphaeria nodorum

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase) By similarity.
Catalytic activity	Carotenoid psi-end group = carotenoid beta-end group. 2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; beta-carotene biosynthesis. Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the lycopene beta-cyclase family. In the C-terminal section; belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC intramolecular lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Bifunctional lycopene cyclase/phytoene synthase		PRO_0000409238
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	75 – 97	23	Helical; Potential
	Transmembrane	123 – 141	19	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Region	1 – 243	243	Lycopene beta-cyclase
	Region	250 – 585	336	Phytoene synthase

Sequences

	Sequence		Length	Mass (Da)
	Q0V6M5 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: B2689322C5E59549	FASTA	585	66,047
	10 20 30 40 50 60 MGFDYAIVHV KYTIPPAVLL TLLYRPLFTK LDAFKVLFLV TVAVTATIPW DSYLIRTNIW 70 80 90 100 110 120 SYPDHVVIGP TLLDIPLEEV FFFFIQTYNT TLLYLILSKP TFQPAYLRAG RPTASSPWKY 130 140 150 160 170 180 QKLAGQLFLV GATVWAGLRV HENAKGTYTG LIVVWAAPII LLQWTLAYQF ILGLPWTNTV 190 200 210 220 230 240 LPIAIPTLYL WLVDTLALRR GTWVISPGTK YGVHLWDGLE IEEALFFFVT NTLIVFGQLA 250 260 270 280 290 300 FDNALAVLYT FPALFPKPPS MPTPLDLINA LWVSPYKYDR ARLAGLQDAV LRLKRKSRSF 310 320 330 340 350 360 YLASATFPGP LRSDLLLLYS FCRVADDLVD NAATAEEAKE WISKLHQYLD LVYSDAKSST 370 380 390 400 410 420 VSEDFVQAHF PSDARSALLQ LPAHKLPRQP LQDLLHGFEM DLAFNTSSPI KTETDLRLYS 430 440 450 460 470 480 ERVAGTVAQM CIELIFRLYP SNMTSGEERK VVDAGNQMGM ALQYVNIARD ISVDAHIGRV 490 500 510 520 530 540 YLPLDWLQES GLTYDEVLIS PEGARMESLR MRLLEKAFSI YDGARGAIET LPVEARGPIR 550 560 570 580 VAVESYMEIG RTLRQKGYTV RAGRATVSKW RRVIVAWRTL NKSIA

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445325 Genomic DNA. Translation: EAT91834.1.
RefSeq	XP_001791029.1. XM_001790977.1.
3D structure databases
ProteinModelPortal	Q0V6M5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SNOT_00339; SNOT_00339; SNOG_00339.
GeneID	5968524.
KEGG	pno:SNOG_00339.
Phylogenomic databases
OMA	VAVESYM.
OrthoDB	EOG4HQHST.
Enzyme and pathway databases
UniPathway	UPA00799; UER00773. UPA00802.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR017825. Lycopene_cyclase_dom. IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03462. CarR_dom_SF. 2 hits.
PROSITE	PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCPS_PHANO

Accession

Primary (citable) accession number: Q0V6M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	September 5, 2006
Last modified:	March 6, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program