Q0V1P1 (AT151_PHANO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase ATG15-1
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:	ATG15-1
ORF Names:	SNOG_02073

Organism

Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)

Taxonomic identifier

321614 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	594 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 594	594	Putative lipase ATG15-1		PRO_0000317968
Regions
	Topological domain	1 – 12	12	Cytoplasmic By similarity
	Transmembrane	13 – 33	21	Helical; Signal-anchor for type II membrane protein
	Topological domain	34 – 594	561	Lumenal By similarity
	Compositional bias	447 – 511	65	Thr-rich
Sites
	Active site	299	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	283	1	N-linked (GlcNAc...) Potential
	Glycosylation	432	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
	Glycosylation	576	1	N-linked (GlcNAc...) Potential
	Glycosylation	582	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0V1P1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: F8F5746260553008	FASTA	594	66,120
	10 20 30 40 50 60 MRRRPLCTSA SRVTASLLLS FLAVSSAAEL PILPAPPISP QPHSSEKDFS LRHIFHHGTY 70 80 90 100 110 120 KYPELHRRLD VPENAAVWAA EHLHSEHREP VPRLRVKAEP MSIQRLADRS KEAIDGILEW 130 140 150 160 170 180 GRMKGRAVQL AEDDWTIDEI AGPNVTDRET VLSFARMASN AYILEPNTGE WEDVGGGFNY 190 200 210 220 230 240 TEDFGWESDG LRGHIFADTE NSTVVIGLKG TSPAMFDGSE TTTKDKENDN LFFSCCCGQG 250 260 270 280 290 300 GQFLWRQVCD CQTSAYTCNS TCLVTALREK NRYYYAAQDL YHNVTALYPH AEIWMAGHSL 310 320 330 340 350 360 GGAVSSFLSL TFGHPAVTFE AVPEAMPASR LGLPVPPGHE IGSLQKRKMT GGYHFGHTAD 370 380 390 400 410 420 PIYMGQCNQA TSVCTFGGYA LQSVCHTGKK CVYDTVKDLG WRVGIGTHKI VEVIKDVIEK 430 440 450 460 470 480 YDAPPICEPY INCTDCYTWK YFESNGTETT TTSTSKPTST SKSSKSNTRT RTETCKTPGW 490 500 510 520 530 540 WGCLDETTTG TQTSTSTPKH TSTSSTSTCK TPGWFGCKGR QRRANHNNNK VLAHNYPRPS 550 560 570 580 590 THRNNNILFL PNINFFMSVP RLVRRLSRRR RPSLPNKTEV VNSSANHFVY VLHA

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445327 Genomic DNA. Translation: EAT90285.1.
RefSeq	XP_001792692.1. XM_001792640.1.
3D structure databases
ProteinModelPortal	Q0V1P1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SNOT_02073; SNOT_02073; SNOG_02073.
GeneID	5969542.
KEGG	pno:SNOG_02073.
Phylogenomic databases
OMA	EFTLRHI.
OrthoDB	EOG4F7RV2.
PhylomeDB	Q0V1P1.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AT151_PHANO

Accession

Primary (citable) accession number: Q0V1P1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 5, 2006
Last modified:	November 16, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program