Reviewed, UniProtKB/Swiss-Prot Q0UY53 (ATG26_PHANO)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	ATG26
ORF Names:	SNOG_03311

Organism

Phaeosphaeria nodorum (Glume blotch fungus) (Septoria nodorum) [Complete proteome]

Taxonomic identifier

13684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	1453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.
Sequence caution	The sequence EAT90042.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1453	1453	Sterol 3-beta-glucosyltransferase		PRO_0000318046
Regions
	Domain	235 – 282	48	GRAM 1
	Domain	286 – 383	98	PH
	Domain	771 – 837	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	Q0UY53-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 6778677005A7FD2D	FASTA	1,453	163,032
	10 20 30 40 50 60 MASQDRGRDR SSRRLTKKRK DIGRDTRGLS LDIPERFKDG DDAQEDVTAP KRSDAMSMNQ 70 80 90 100 110 120 SIFSIIARAG GQSQVDLGTM QEVDSGDSDD DGKGKTLHDR LDGAARLSRL RTANDFRPPQ 130 140 150 160 170 180 EDSRAEKIGS GKHRRALSEN KLLRSLPKLK ISSRKDVRSD GQHTDQMSSS QFLPPRPSTE 190 200 210 220 230 240 TPSETHKRGD ALKGKARATT GDEIHVEKQR KSERRGGQGS SVGLAQSRGP VSLATRIQQI 250 260 270 280 290 300 FEFEHAEEVI SEYPCWLLQS ILLQGYMYIT QKHICFYAYI PKKHHDVSKT GYLYKRGRSK 310 320 330 340 350 360 YNRYWFILRG DVLSYYTNPA ELYFPRNRIN LQYAISAEVV ENKKKGEYET IFTVTTDERT 370 380 390 400 410 420 YQFKADSAAS AKEWVKSIQK VIFRTHNEGN SVKISMPIRN VIEIEESSIL DFADTVKVRV 430 440 450 460 470 480 VDNGETFAID EYFFSFFNRG QDALNVLRIM INDNEHHQVS TENQVNPTPI TAPLKTSNRH 490 500 510 520 530 540 SLVVSPIVPD DTPRIKENVR ATLSPLPNPN VGRSSISDIS ARSSTDVPRK SWDVRRSMDA 550 560 570 580 590 600 SRALRRSSHE VRRSFSGGRS EKLTKSRAPD RSPLSPAADT ESATLSLDPG TESSAAIQSM 610 620 630 640 650 660 DESSASASQI LNRSDVFRAP AIHSAGVSHS ERPQQAARHS QDTTRSSGAD QHLQLLVRPS 670 680 690 700 710 720 KQTNTSLLPA STEAEEDTDP SQTLPRLSNS SSALQEIASY PLQKASGIAG FLRMRSKEMG 730 740 750 760 770 780 SLLATESMGY YEKVSGMLAG GRKHYNTADG LEPHDQIHGI EDDDDAAKAA DNFREHFAFP 790 800 810 820 830 840 ESEILQSSFF ASLQRMLPNY GKIYISGRYF CFRSLMPTSK TKIILPMKDI ENVNKEKGFR 850 860 870 880 890 900 MGYHGLAIVI RGHEELFFEF SKAEYRDECA ITVLRILENT KYIEEASSSS GVDSDDEAAK 910 920 930 940 950 960 AEHDLLQQAR EQSDTHEQID VSEIARAADH DRIPLIFDDP LASFVDFKPP EPLTIVCLTI 970 980 990 1000 1010 1020 GSRGDVQPYI SLCKELLKEG HKARIATHAE FEPWVRKHGI DFAPVDGDPA ELMRICVEHG 1030 1040 1050 1060 1070 1080 MFTYGFIKEA NSKFRGWLDD VCSSSWRACQ GADVLIESPS TMAGIHVAEA LEIPYFRAFT 1090 1100 1110 1120 1130 1140 MPWSRTRAYP HAFSVLENKM GGGYNNMTYQ LFDKLFWTAI SAQVNKWRRR ELGLQNTSQS 1150 1160 1170 1180 1190 1200 KMQANLRPFL YNFSPHVVPP PLDWPDWIRV TGYWFLDEGD TYQPPADLAA FITQARKDEK 1210 1220 1230 1240 1250 1260 KLVYVGFGSI VIDDPAALTK TVVDSVLKAD VRCVLSKGWS DRLATKDASK PEIPLPPEIF 1270 1280 1290 1300 1310 1320 QIQAAPHDWL FKQMDAAVHH GGSGTTGASL RAGIPTIIKP FFGDQFFFAQ RVEDMGVGIW 1330 1340 1350 1360 1370 1380 LKKVNTSVFS RALWEATNSQ RMIIKAKVLG QKIRKDNGPQ VAIQTIYREL DRARTLVKKH 1390 1400 1410 1420 1430 1440 VKQDDEQTDE FEEDWTMIEE GQDVDVSAHH FEAQSPLVGM NQDAPRTGGG SLVLGSMVMK 1450 GAQKRNSESA YRD

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH445328 Genomic DNA. Translation: EAT90042.2. Sequence problems.
3D structure databases
ModBase	Search...
Phylogenomic databases
OMA	THVLFEN.
Enzyme and pathway databases
BRENDA	2.4.1.173. 281328.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_PHANO

Accession

Primary (citable) accession number: Q0UY53

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)