Q0UEY4 (NCB5R_PHANO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 1
EC=1.6.2.2

Alternative name(s):
Microsomal cytochrome b reductase

Gene names

Name:	CBR1
ORF Names:	SNOG_09680

Organism

Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)

Taxonomic identifier

321614 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity. Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	NADH-cytochrome b5 reductase 1		PRO_0000330161
Regions
	Transmembrane	2 – 22	21	Helical; Potential
	Domain	36 – 141	106	FAD-binding FR-type
	Nucleotide binding	121 – 136	16	FAD By similarity
	Nucleotide binding	147 – 181	35	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0UEY4 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 42BC818575EBE25E	FASTA	282	30,777
	10 20 30 40 50 60 MAFSPQALLP YVVLFVAAVG GWKVYSSGSK KVLKPNEFQE FELEEKTVLS HNTAIYRFKL 70 80 90 100 110 120 PRKTDILGLP IGQHISLGAT IEGQPKEVVR SYTPISSDED KGHFDLLIKS YPTGNISKYV 130 140 150 160 170 180 ANLKVGEKMR VKGPKGAMVY TPNMVRHFGM ISGGTGITPM LQVVKAIIRG RGQGDTTEVD 190 200 210 220 230 240 LIFANVNPED ILLKEDLDAL AAKDPKFRVH YVLNNPPEGW TGGVGFVTAD MIKEKLPAPA 250 260 270 280 SDVKILVCGP PPMVAAMKKA TESLGYAKAK PVSKLEDQVF CF

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445339 Genomic DNA. Translation: EAT82945.2.
RefSeq	XP_001799967.1. XM_001799915.1.
3D structure databases
ProteinModelPortal	Q0UEY4.
SMR	Q0UEY4. Positions 30-268.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SNOT_09680; SNOT_09680; SNOG_09680.
GeneID	5976872.
KEGG	pno:SNOG_09680.
Phylogenomic databases
OrthoDB	EOG4FFH9R.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_PHANO

Accession

Primary (citable) accession number: Q0UEY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 5, 2008
Last modified:	November 16, 2011
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program