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Protein

DNA ligase 4

Gene

LIG4

Organism
Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining.By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate.PROSITE-ProRule annotation

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei324ATPBy similarity1
Active sitei326N6-AMP-lysine intermediatePROSITE-ProRule annotation1
Binding sitei331ATPBy similarity1
Binding sitei353ATPBy similarity1
Metal bindingi394Magnesium 1Sequence analysis1
Metal bindingi496Magnesium 2Sequence analysis1
Binding sitei501ATPBy similarity1
Binding sitei512ATPBy similarity1
Binding sitei518ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:LIG4
ORF Names:SNOG_10525
OrganismiPhaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)
Taxonomic identifieri321614 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePhaeosphaeriaceaeParastagonospora
Proteomesi
  • UP000001055 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002783851 – 990DNA ligase 4Add BLAST990

Structurei

3D structure databases

ProteinModelPortaliQ0UCI9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini728 – 821BRCT 1PROSITE-ProRule annotationAdd BLAST94
Domaini900 – 989BRCT 2PROSITE-ProRule annotationAdd BLAST90

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.Curated
Contains 2 BRCT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiQ0UCI9.
KOiK10777.
OMAiHPYEEAT.
OrthoDBiEOG092C18KW.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 3 hits.
PfamiPF16589. BRCT_2. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0UCI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEDTEMPDA EAVRENTLMY GHDDELDEKF PTRPMNLHKS PPFHTLFTDL
60 70 80 90 100
FDPLMETQKK GRQPPGPRRK AGPHGHSNLS PHEAKRNIIE RFIASWRKTV
110 120 130 140 150
GNDFYPAMRL IIPDKDRDRA MYGLKEKAIA KVLIKLTKIS KDSDDAKQML
160 170 180 190 200
NWKLPGQLHK ASASTAGDFA GRCYQVLSSR QLNTKLGDMS IAEVNNALDK
210 220 230 240 250
LSQLGSEDEQ VKIFQRFNRR MNAEEMTWLI RIILRQMKIG ATEKTFLDIW
260 270 280 290 300
HPDAETLFNI SSNLRRVCWE LYDPKVRLEG EDTGLSLMQC FQPQLAAFQD
310 320 330 340 350
KGGSFEKIVA RLQANSDDDS FWIEEKLDGE RMQLHMIEDP EAPGGKLFGF
360 370 380 390 400
WSRKAKDYAY LYGKHLQGDE AGALTRFITD AFGKNVRNII LDGEMITWDM
410 420 430 440 450
DVDHIVGFGT LKTAAISEKE NKTDKSTGQR PLFRVFDCVY LNDKLLTPYT
460 470 480 490 500
LRDRRRALES AVKDVKRRLE IHPYIEAHSH TEIEPALRKV VAESSEGLVL
510 520 530 540 550
KNPRSMYRLN DRNADWMKVK PEYMSEFGES LDCIVVGGYF GSGHRGGAHS
560 570 580 590 600
SFLCALLLNK DAKPGDADYE KCWSFFKVGG GFSREDYAAI RGRTEGKWKD
610 620 630 640 650
WDPRRPPPII ELGGHEQNRQ HERPDQWIHP SDSVVLECKA ASVEGSDKFR
660 670 680 690 700
FNLTLRFPRF RMLRTDKRWD QALSREEFYE IKANVEYKRE EKEKEFEIEK
710 720 730 740 750
SRRKRTRTTK KPIVVAGSET ITTPYAGPQS KIFDGLSFYI MTEQLHPTKK
760 770 780 790 800
SKVDLEALVK ANGGRVVQRD SMEPNLVIVA DKRLIKVASL EKRDTNNIVK
810 820 830 840 850
PVWIQDTIQQ NEVDNGALPY LLPFEPNRHM FYLLGDNQLD YEANADDYGD
860 870 880 890 900
SYARDIADVE EMRKILGATD PPQKRTKFDR ESFLDQLEDH GDSLSHLKTY
910 920 930 940 950
MFSSTKVAFR TSDDPVWTLR AQLAGNYIRF GGGQITEDED EEGVTHTVVP
960 970 980 990
DGQTASVSRV ADLSRVVGVG WVQKCWDEST RLDEERYQWG
Length:990
Mass (Da):113,591
Last modified:September 5, 2006 - v1
Checksum:i8B9386A5E66CAA50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH445341 Genomic DNA. Translation: EAT81919.1.
RefSeqiXP_001800794.1. XM_001800742.1.

Genome annotation databases

EnsemblFungiiSNOT_10525; SNOT_10525; SNOG_10525.
GeneIDi5977701.
KEGGipno:SNOG_10525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH445341 Genomic DNA. Translation: EAT81919.1.
RefSeqiXP_001800794.1. XM_001800742.1.

3D structure databases

ProteinModelPortaliQ0UCI9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSNOT_10525; SNOT_10525; SNOG_10525.
GeneIDi5977701.
KEGGipno:SNOG_10525.

Phylogenomic databases

InParanoidiQ0UCI9.
KOiK10777.
OMAiHPYEEAT.
OrthoDBiEOG092C18KW.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR029710. LIG4.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PANTHERiPTHR10459:SF7. PTHR10459:SF7. 3 hits.
PfamiPF16589. BRCT_2. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNLI4_PHANO
AccessioniPrimary (citable) accession number: Q0UCI9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: September 5, 2006
Last modified: November 30, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.