Reviewed, UniProtKB/Swiss-Prot Q0UCI9 (DNLI4_PHANO)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
ORF Names:	SNOG_10525

Organism

Phaeosphaeria nodorum (Septoria nodorum) [Complete proteome]

Taxonomic identifier

13684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	990 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 990	990	DNA ligase 4		PRO_0000278385
Regions
	Domain	728 – 821	94	BRCT 1
	Domain	900 – 989	90	BRCT 2
Sites
	Active site	326	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0UCI9-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 8B9386A5E66CAA50	FASTA	990	113,591
	10 20 30 40 50 60 MAEDTEMPDA EAVRENTLMY GHDDELDEKF PTRPMNLHKS PPFHTLFTDL FDPLMETQKK 70 80 90 100 110 120 GRQPPGPRRK AGPHGHSNLS PHEAKRNIIE RFIASWRKTV GNDFYPAMRL IIPDKDRDRA 130 140 150 160 170 180 MYGLKEKAIA KVLIKLTKIS KDSDDAKQML NWKLPGQLHK ASASTAGDFA GRCYQVLSSR 190 200 210 220 230 240 QLNTKLGDMS IAEVNNALDK LSQLGSEDEQ VKIFQRFNRR MNAEEMTWLI RIILRQMKIG 250 260 270 280 290 300 ATEKTFLDIW HPDAETLFNI SSNLRRVCWE LYDPKVRLEG EDTGLSLMQC FQPQLAAFQD 310 320 330 340 350 360 KGGSFEKIVA RLQANSDDDS FWIEEKLDGE RMQLHMIEDP EAPGGKLFGF WSRKAKDYAY 370 380 390 400 410 420 LYGKHLQGDE AGALTRFITD AFGKNVRNII LDGEMITWDM DVDHIVGFGT LKTAAISEKE 430 440 450 460 470 480 NKTDKSTGQR PLFRVFDCVY LNDKLLTPYT LRDRRRALES AVKDVKRRLE IHPYIEAHSH 490 500 510 520 530 540 TEIEPALRKV VAESSEGLVL KNPRSMYRLN DRNADWMKVK PEYMSEFGES LDCIVVGGYF 550 560 570 580 590 600 GSGHRGGAHS SFLCALLLNK DAKPGDADYE KCWSFFKVGG GFSREDYAAI RGRTEGKWKD 610 620 630 640 650 660 WDPRRPPPII ELGGHEQNRQ HERPDQWIHP SDSVVLECKA ASVEGSDKFR FNLTLRFPRF 670 680 690 700 710 720 RMLRTDKRWD QALSREEFYE IKANVEYKRE EKEKEFEIEK SRRKRTRTTK KPIVVAGSET 730 740 750 760 770 780 ITTPYAGPQS KIFDGLSFYI MTEQLHPTKK SKVDLEALVK ANGGRVVQRD SMEPNLVIVA 790 800 810 820 830 840 DKRLIKVASL EKRDTNNIVK PVWIQDTIQQ NEVDNGALPY LLPFEPNRHM FYLLGDNQLD 850 860 870 880 890 900 YEANADDYGD SYARDIADVE EMRKILGATD PPQKRTKFDR ESFLDQLEDH GDSLSHLKTY 910 920 930 940 950 960 MFSSTKVAFR TSDDPVWTLR AQLAGNYIRF GGGQITEDED EEGVTHTVVP DGQTASVSRV 970 980 990 ADLSRVVGVG WVQKCWDEST RLDEERYQWG

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH445341 Genomic DNA. Translation: EAT81919.1.
RefSeq	XP_001800794.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5977701.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_PHANO

Accession

Primary (citable) accession number: Q0UCI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	September 5, 2006
Last modified:	November 25, 2008
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)