DNA ligase 4 - Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus)

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA ligase 4
EC=6.5.1.1

Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
ORF Names:	SNOG_10525

Organism

Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum) [Reference proteome]

Taxonomic identifier

321614 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Phaeosphaeriaceae › Phaeosphaeria ›

Protein attributes

Sequence length	990 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 990

990

DNA ligase 4

PRO_0000278385

Regions

Domain

728 – 821

94

BRCT 1

Domain

900 – 989

90

BRCT 2

Sites

Active site

326

1

N6-AMP-lysine intermediate By similarity

Metal binding

394

1

Magnesium 1 Potential

Metal binding

496

1

Magnesium 2 Potential

Binding site

324

1

ATP By similarity

Binding site

331

1

ATP By similarity

Binding site

353

1

ATP By similarity

Binding site

501

1

ATP By similarity

Binding site

512

1

ATP By similarity

Binding site

518

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q0UCI9 [UniParc].

Last modified September 5, 2006. Version 1.
Checksum: 8B9386A5E66CAA50
Show »

FASTA

990

113,591

        10         20         30         40         50         60 
MAEDTEMPDA EAVRENTLMY GHDDELDEKF PTRPMNLHKS PPFHTLFTDL FDPLMETQKK 

        70         80         90        100        110        120 
GRQPPGPRRK AGPHGHSNLS PHEAKRNIIE RFIASWRKTV GNDFYPAMRL IIPDKDRDRA 

       130        140        150        160        170        180 
MYGLKEKAIA KVLIKLTKIS KDSDDAKQML NWKLPGQLHK ASASTAGDFA GRCYQVLSSR 

       190        200        210        220        230        240 
QLNTKLGDMS IAEVNNALDK LSQLGSEDEQ VKIFQRFNRR MNAEEMTWLI RIILRQMKIG 

       250        260        270        280        290        300 
ATEKTFLDIW HPDAETLFNI SSNLRRVCWE LYDPKVRLEG EDTGLSLMQC FQPQLAAFQD 

       310        320        330        340        350        360 
KGGSFEKIVA RLQANSDDDS FWIEEKLDGE RMQLHMIEDP EAPGGKLFGF WSRKAKDYAY 

       370        380        390        400        410        420 
LYGKHLQGDE AGALTRFITD AFGKNVRNII LDGEMITWDM DVDHIVGFGT LKTAAISEKE 

       430        440        450        460        470        480 
NKTDKSTGQR PLFRVFDCVY LNDKLLTPYT LRDRRRALES AVKDVKRRLE IHPYIEAHSH 

       490        500        510        520        530        540 
TEIEPALRKV VAESSEGLVL KNPRSMYRLN DRNADWMKVK PEYMSEFGES LDCIVVGGYF 

       550        560        570        580        590        600 
GSGHRGGAHS SFLCALLLNK DAKPGDADYE KCWSFFKVGG GFSREDYAAI RGRTEGKWKD 

       610        620        630        640        650        660 
WDPRRPPPII ELGGHEQNRQ HERPDQWIHP SDSVVLECKA ASVEGSDKFR FNLTLRFPRF 

       670        680        690        700        710        720 
RMLRTDKRWD QALSREEFYE IKANVEYKRE EKEKEFEIEK SRRKRTRTTK KPIVVAGSET 

       730        740        750        760        770        780 
ITTPYAGPQS KIFDGLSFYI MTEQLHPTKK SKVDLEALVK ANGGRVVQRD SMEPNLVIVA 

       790        800        810        820        830        840 
DKRLIKVASL EKRDTNNIVK PVWIQDTIQQ NEVDNGALPY LLPFEPNRHM FYLLGDNQLD 

       850        860        870        880        890        900 
YEANADDYGD SYARDIADVE EMRKILGATD PPQKRTKFDR ESFLDQLEDH GDSLSHLKTY 

       910        920        930        940        950        960 
MFSSTKVAFR TSDDPVWTLR AQLAGNYIRF GGGQITEDED EEGVTHTVVP DGQTASVSRV 

       970        980        990 
ADLSRVVGVG WVQKCWDEST RLDEERYQWG

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References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum."
Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P.
Plant Cell 19:3347-3368(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445341 Genomic DNA. Translation: EAT81919.1.
RefSeq	XP_001800794.1. XM_001800742.1.
3D structure databases
ProteinModelPortal	Q0UCI9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SNOT_10525; SNOT_10525; SNOG_10525.
GeneID	5977701.
KEGG	pno:SNOG_10525.
Phylogenomic databases
KO	K10777.
OMA	HMCPSTK.
OrthoDB	EOG41K2MD.
Family and domain databases
Gene3D	1.10.3260.10. 1 hit. 2.40.50.140. 1 hit. 3.40.50.10190. 2 hits.
InterPro	IPR001357. BRCT_dom. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. [Graphical view]
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
SUPFAM	SSF52113. BRCT. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_PHANO

Accession

Primary (citable) accession number: Q0UCI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	September 5, 2006
Last modified:	May 29, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents