Reviewed, UniProtKB/Swiss-Prot Q0U9W5 (MCR1_PHANO)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
    EC=1.6.2.2
Alternative name(s):
    Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
ORF Names:	SNOG_11449

Organism

Phaeosphaeria nodorum (Glume blotch fungus) (Septoria nodorum) [Complete proteome]

Taxonomic identifier

13684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	NADH-cytochrome b5 reductase 2		PRO_0000330187
Regions
	Transmembrane	28 – 50	23	Potential
	Domain	84 – 189	106	FAD-binding FR-type
	Nucleotide binding	192 – 227	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0U9W5-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: D6BDA6CF8E11601B	FASTA	336	37,024
	10 20 30 40 50 60 MFARQVIRPA RQLQQHVRRY ASEAPQSGGS SNGALYVGIG AAGLAGAYIY MRGGKPAAPL 70 80 90 100 110 120 SEEANKVAAK VGGASKKAFT GGDQGFISLL LDKSEVVNHN TKKLTFKLPE PDMESGLPVT 130 140 150 160 170 180 SAVITKYKGP EMEKPVIRPY TPVSDVDQQG TVDFIVKKYE KGPMSSHMHN MEPGQRLDIK 190 200 210 220 230 240 GPIPKYPWSP NKHEHIALIA GGTGITPMWQ TARAIFKNPE DKTKVTLVFG NISEEDILLK 250 260 270 280 290 300 KEWEHLENTY PQRFRAFYVL DNPPESWQGG KGFITKELLK TVLPEPKEGE KVKIFVCGPP 310 320 330 GMYKAISGGK KSPSDQGELD GYLKELGYSK DQVYKF

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH445343 Genomic DNA. Translation: EAT81157.1.
RefSeq	XP_001801691.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5978599.
Phylogenomic databases
OMA	YANVSPD.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_PHANO

Accession

Primary (citable) accession number: Q0U9W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)