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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

SNOG_12368

Organism
Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511PhosphateUniRule annotation
Sitei216 – 2161Important for substrate specificityUniRule annotation
Binding sitei234 – 2341Substrate; via amide nitrogenUniRule annotation
Binding sitei235 – 2351PhosphateUniRule annotation
Sitei272 – 2721Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. mRNA binding Source: EnsemblFungi
  2. phosphorylase activity Source: InterPro
  3. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutamate biosynthetic process Source: EnsemblFungi
  2. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  3. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:SNOG_12368
OrganismiPhaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)
Taxonomic identifieri321614 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePhaeosphaeriaceaeParastagonospora
ProteomesiUP000001055: Unassembled WGS sequence

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344S-methyl-5'-thioadenosine phosphorylasePRO_0000415132Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ0U796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1002Phosphate bindingUniRule annotation
Regioni132 – 1332Phosphate bindingUniRule annotation
Regioni258 – 2603Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiQ0U796.
KOiK00772.
OrthoDBiEOG77DJGM.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0U796-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLQRARLWPP IFTSRCAPFP FSRSISIPYR RMAGVPSTYD APVHIAVIGG
60 70 80 90 100
TGISSLPGFE LAATLDVDTP WGKPSSPISI LQHNSPTTGK PVPVAFLSRH
110 120 130 140 150
GLHHEHAPHE VKNQANIAAL RHIGVRTIIA FSAVGSLQEE VRPRDFVVPD
160 170 180 190 200
QIIDRTKGIR PFTFFEKGMV GHVGFGDPFD KSLAEIVRKC GHSLEGEGVR
210 220 230 240 250
LHDKGLLICM EGPQFSTRAE SNLYRTWGGS VINMSALPEA KLAREAEISY
260 270 280 290 300
QMICMATDYD CWRGDGSEDV NVEMVMAHMK ANAENARRFV GAVLNELTKE
310 320 330 340
EHGELVLAKH IEGQMRFAGA MTKKEGRGQD AEKKLQWLFP GYFD
Length:344
Mass (Da):38,059
Last modified:February 5, 2008 - v2
Checksum:i2BA85D8DF4E3577A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH445346 Genomic DNA. Translation: EAT80181.2.
RefSeqiXP_001802590.1. XM_001802538.1.

Genome annotation databases

EnsemblFungiiSNOT_12368; SNOT_12368; SNOG_12368.
GeneIDi5979499.
KEGGipno:SNOG_12368.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH445346 Genomic DNA. Translation: EAT80181.2.
RefSeqiXP_001802590.1. XM_001802538.1.

3D structure databases

ProteinModelPortaliQ0U796.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSNOT_12368; SNOT_12368; SNOG_12368.
GeneIDi5979499.
KEGGipno:SNOG_12368.

Phylogenomic databases

InParanoidiQ0U796.
KOiK00772.
OrthoDBiEOG77DJGM.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum."
    Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P.
    Plant Cell 19:3347-3368(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Entry informationi

Entry nameiMTAP_PHANO
AccessioniPrimary (citable) accession number: Q0U796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: February 5, 2008
Last modified: January 7, 2015
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.