Reviewed, UniProtKB/Swiss-Prot Q0U6E8 (MTLD_PHANO)

Names and origin

Protein names

Recommended name:
    Mannitol-1-phosphate 5-dehydrogenase
      Short name=M1PDH
      Short name=MPDH
      Short name=MPD
    EC=1.1.1.17

Gene names

Name:	mpd1
ORF Names:	SNOG_12666

Organism

Phaeosphaeria nodorum (Glume blotch fungus) (Septoria nodorum) [Complete proteome]

Taxonomic identifier

13684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the NAD(H)-dependent interconversion of D-fructose 6-phosphate and D-mannitol 1-phosphate in the mannitol metabolic pathway. Required for the process of sporulation on senescing leaf material. Ref.1 Ref.4
Catalytic activity	D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the mannitol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol-1-phosphate 5-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Mannitol-1-phosphate 5-dehydrogenase		PRO_0000371534
Regions
	Nucleotide binding	7 – 18	12	NAD By similarity
Sites
	Active site	216	1	By similarity
Experimental info
	Sequence conflict	101	1	F → L in AAT11122. Ref.1
	Sequence conflict	295	1	E → K in AAT11122. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q0U6E8-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: 272D3357DEDE8278	FASTA	390	43,475
	10 20 30 40 50 60 MPYDKKAVHF GGGNIGRGFV AEFLHNSGYE VVFVDVMDSI IEALQKQSSY TVTEIGDDGE 70 80 90 100 110 120 REFTIDHYRA LNSKHEMDKV VQEIASADVV TCAVGPNILK FVAEPVAKAI DARTLDYPIA 130 140 150 160 170 180 VIACENAINA TTTWRGFIEG KLSEDSKSNL DKKARFANSA IDRIVPVQDK DAGLNVKIEK 190 200 210 220 230 240 FYEWCVEQKP FENGGKKPDV KGIHYVDDLE PYIERKLFTV NTSHATAAYY GHQAKKQYIH 250 260 270 280 290 300 EVLQDKKLHD IVRDAVKETA HLIVSKHGVS VQEQNDYVDS IIKRISNPVL KDNVERVGRA 310 320 330 340 350 360 PLRKLSRKER FVGPAAQLAE RGEKVDALLG AIEQAYLFQN VEGDEESAEL AKILKENSAE 370 380 390 EVVTKVNGLD KSHPLFEKIL PIVKKVQGGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mannitol 1-phosphate metabolism is required for sporulation in planta of the wheat pathogen Stagonospora nodorum." Solomon P.S., Tan K.-C., Oliver R.P. Mol. Plant Microbe Interact. 18:110-115(2005) [PubMed: 15720079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: SN15.
[2]	"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15.
[3]	"Genetic mapping of Phaeosphaeria nodorum." Malkus A., Chiu E.Y., Ueng P.P. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-389. Strain: S-81-B13B and Sn37-1.
[4]	"Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch)." Solomon P.S., Waters O.D.C., Joergens C.I., Lowe R.G.T., Rechberger J., Trengove R.D., Oliver R.P. Biochem. J. 399:231-239(2006) [PubMed: 16859492] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY547308 mRNA. Translation: AAT11122.1. CH445347 Genomic DNA. Translation: EAT79964.1. FJ151548 Genomic DNA. Translation: ACI04508.1. FJ151549 Genomic DNA. Translation: ACI04509.1.
RefSeq	XP_001802887.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5979798.
Phylogenomic databases
OMA	VDRIVPN.
Family and domain databases
InterPro	IPR013328. DH_multihelical. IPR013118. Mannitol_DH_C. IPR000669. Mannitol_DH_core. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
PROSITE	PS00974. MANNITOL_DHGENASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLD_PHANO

Accession

Primary (citable) accession number: Q0U6E8
Secondary accession number(s): B6DQL1, Q5DQ93

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	September 5, 2006
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)