Q0U5H7 (CARA_PHANO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase arginine-specific small chain
Short name=CPS-A
EC=6.3.5.5

Alternative name(s):
Arginine-specific carbamoyl-phosphate synthetase, glutamine chain

Gene names

Name:	CPA1
ORF Names:	SNOG_12987

Organism

Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)

Taxonomic identifier

321614 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Carbamoyl-phosphate synthase arginine-specific small chain		PRO_0000290598
Regions
	Domain	228 – 415	188	Glutamine amidotransferase type-1
Sites
	Active site	304	1	Nucleophile By similarity
	Active site	388	1	By similarity
	Active site	390	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0U5H7 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: AEBD2D76D5F792E3	FASTA	476	52,138
	10 20 30 40 50 60 MFSHLLKPAA RSAGLLGHVN RRYLATVHTN TAREIPKPSR KPTPISLENA TFTIKNGPIF 70 80 90 100 110 120 SGKSFGAKAN ISGEAVFTTS LVGYPESMTD PSYRGQILVF TQPLIGNYGV PSSARDEHGL 130 140 150 160 170 180 LRYFESPNIQ ASGIVVQDYA LKHSHWTAVE SLAQWCAREG VPAISGVDTR EVVTYLREQG 190 200 210 220 230 240 SSLARITVGE EYDADEDEAY IDPEAINLVR RVSTKAPFHV SSSLGDMHVA LIDCGVKENI 250 260 270 280 290 300 LRSLVSRGAS VTCFPFDYPI HKVAHHFDGV FISNGPGDPT HCTSTVYNLR KLFETSQLPV 310 320 330 340 350 360 MGICMGHQLI ALAAGAKTIK LKYGNRAHNI PALDLTTGKC HITSQNHGYA VDPTTLTSEW 370 380 390 400 410 420 KEYFTNLNDQ SNEGLIHASR PIFSAQFHPE AKGGPMDSSY LFDKYIQNVQ RYKDHQSSFS 430 440 450 460 470 EKSNKPSPLL VDLLSKERVG VHPAQPDFEM HVPGRVEQVD VGGPVAPPYQ PITAAA

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445348 Genomic DNA. Translation: EAT79787.1.
RefSeq	XP_001803201.1. XM_001803149.1.
3D structure databases
ProteinModelPortal	Q0U5H7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SNOT_12987; SNOT_12987; SNOG_12987.
GeneID	5980114.
KEGG	pno:SNOG_12987.
Phylogenomic databases
OMA	QNHSYVV.
OrthoDB	EOG4N8VDH.
PhylomeDB	Q0U5H7.
Family and domain databases
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_PHANO

Accession

Primary (citable) accession number: Q0U5H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	September 5, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program