Reviewed, UniProtKB/Swiss-Prot Q0U5H7 (CARA_PHANO)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase arginine-specific small chain
      Short name=CPS-A
    EC=6.3.5.5
Alternative name(s):
    Arginine-specific carbamoyl-phosphate synthetase, glutamine chain

Gene names

Name:	CPA1
ORF Names:	SNOG_12987

Organism

Phaeosphaeria nodorum (Septoria nodorum) [Complete proteome]

Taxonomic identifier

13684 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1.
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Carbamoyl-phosphate synthase arginine-specific small chain		PRO_0000290598
Regions
	Domain	228 – 415	188	Glutamine amidotransferase type-1
Sites
	Active site	304	1	Nucleophile By similarity
	Active site	388	1	By similarity
	Active site	390	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0U5H7-1 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: AEBD2D76D5F792E3	FASTA	476	52,138
	10 20 30 40 50 60 MFSHLLKPAA RSAGLLGHVN RRYLATVHTN TAREIPKPSR KPTPISLENA TFTIKNGPIF 70 80 90 100 110 120 SGKSFGAKAN ISGEAVFTTS LVGYPESMTD PSYRGQILVF TQPLIGNYGV PSSARDEHGL 130 140 150 160 170 180 LRYFESPNIQ ASGIVVQDYA LKHSHWTAVE SLAQWCAREG VPAISGVDTR EVVTYLREQG 190 200 210 220 230 240 SSLARITVGE EYDADEDEAY IDPEAINLVR RVSTKAPFHV SSSLGDMHVA LIDCGVKENI 250 260 270 280 290 300 LRSLVSRGAS VTCFPFDYPI HKVAHHFDGV FISNGPGDPT HCTSTVYNLR KLFETSQLPV 310 320 330 340 350 360 MGICMGHQLI ALAAGAKTIK LKYGNRAHNI PALDLTTGKC HITSQNHGYA VDPTTLTSEW 370 380 390 400 410 420 KEYFTNLNDQ SNEGLIHASR PIFSAQFHPE AKGGPMDSSY LFDKYIQNVQ RYKDHQSSFS 430 440 450 460 470 EKSNKPSPLL VDLLSKERVG VHPAQPDFEM HVPGRVEQVD VGGPVAPPYQ PITAAA

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH445348 Genomic DNA. Translation: EAT79787.1.
RefSeq	XP_001803201.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5980114.
Phylogenomic databases
OMA	Q0U5H7. EGVPAIS.
Enzyme and pathway databases
BRENDA	6.3.5.5. 281328.
Family and domain databases
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_PHANO

Accession

Primary (citable) accession number: Q0U5H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)