Q0U324 (KATG_PHANO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
ORF Names:	SNOG_13840

Organism

Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)

Taxonomic identifier

321614 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Phaeosphaeriaceae › Phaeosphaeria

Protein attributes

Sequence length	751 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. 2 H2O2 = O2 + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 751	751	Catalase-peroxidase		PRO_0000354109
Sites
	Active site	93	1	Proton acceptor By similarity
	Metal binding	281	1	Iron (heme axial ligand) By similarity
	Site	89	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	92 ↔ 240		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-266) By similarity
	Cross-link	240 ↔ 266		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-92) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0U324 [UniParc]. Last modified September 5, 2006. Version 1. Checksum: FC5E1BFA5C89B85B	FASTA	751	83,498
	10 20 30 40 50 60 MSKGECPMRT ANVAGGGTKI KDWWPNELPV SVLRQHDPRQ NPLTSDFNYA EEFKKLDYNA 70 80 90 100 110 120 LKKDLTALMT DSQDWWPADF GHYGGLFVRM AWHSAGTYRV TDGRGGGGDG QQRFAPLNAW 130 140 150 160 170 180 PDNVSLDKAR RLLWPIKQKY GNKISWADLM LLTGNVALES MGCETFGFAG GRPDTFQSDE 190 200 210 220 230 240 SIYWGGEDTW LGNDVRYSNG NKGVSGEGVV DGDQHKMDHK DIHSRDLEQP VAAAHMGLIY 250 260 270 280 290 300 VNPEGPDGVP DPIAAARDIR TTFHRMAMND EETAALIIGG HSFGKTHGAA PSENTGPDPN 310 320 330 340 350 360 SEDLSTQGFG WINKHGSGKG PDTITSGLEV TWTGTPTKWS NKYLEYLYKY EWELEKSPAG 370 380 390 400 410 420 ANQWVAKTED HIIPDAYDAN KKHKPRMLTT DMSMRMDPGF EKITRRWLDH PQELHDAFIR 430 440 450 460 470 480 AWFKLLHRDM GPRSRWVGPE IPKEVLLWED PVPEVDHALV EESDISALKK QILEAGIEPS 490 500 510 520 530 540 KLIRTAWASA ASYRGSDKRG GANGARIRLA PQKDWEVNNP KELAEVLKAL EGVQSKFNGS 550 560 570 580 590 600 ASGGKKISLA DLIVLAGTAA VEKAAGVSVP FTPGRTDATE EQTDAKSFEH LEPATDPFRN 610 620 630 640 650 660 YGKSSDRART EQLDLDKASL LRLTAPELTV LVGGMRALNA NWDSSSHGIF TKRPGQLTND 670 680 690 700 710 720 FFVNLLDINT EWKAADSSKL PELYEGFDRK SGQKKWTGTR HDLVYGSHPE LRAIAEVYAQ 730 740 750 PDNSDKFVKD FVKAWDKVMS NDRFDLKAKS S

References

[1]

"Dothideomycete-plant interactions illuminated by genome sequencing and EST analysis of the wheat pathogen Stagonospora nodorum." Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L., Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E., Torriani S.F.F., McDonald B.A., Oliver R.P. Plant Cell 19:3347-3368(2007) [PubMed: 18024570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SN15 / ATCC MYA-4574 / FGSC 10173.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH445352 Genomic DNA. Translation: EAT78864.1.
RefSeq	XP_001804042.1. XM_001803990.1.
3D structure databases
ProteinModelPortal	Q0U324.
SMR	Q0U324. Positions 17-746.
ModBase	Search...
Protein family/group databases
PeroxiBase	3449. PnoCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SNOT_13840; SNOT_13840; SNOG_13840.
GeneID	5980964.
KEGG	pno:SNOG_13840.
Phylogenomic databases
OMA	KRHAPSM.
OrthoDB	EOG41CB4B.
PhylomeDB	Q0U324.
Family and domain databases
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_PHANO

Accession

Primary (citable) accession number: Q0U324

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 5, 2006
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program